基于金标银染技术的冈田酸核酸适配体传感器的研究

引用本文: 童萍, 张慧玲, 陆伟, 张文敏, 张兰. 基于金标银染技术的冈田酸核酸适配体传感器的研究[J]. 分析化学, 2022, 50(9): 1355-1363. doi: 10.19756/j.issn.0253-3820.221191 shu

Citation: TONG Ping, ZHANG Hui-Ling, LU Wei, ZHANG Wen-Min, ZHANG Lan. An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label[J]. Chinese Journal of Analytical Chemistry, 2022, 50(9): 1355-1363. doi: 10.19756/j.issn.0253-3820.221191 shu

基于金标银染技术的冈田酸核酸适配体传感器的研究

童萍 ^1,2,3, ,
张慧玲 ^1,2, ,
陆伟 ^3, ,
张文敏 ^3,4, ,
张兰 ^{3,
,}

1.
福州大学福建省高校测试中心, 福州 350116;
2.
福州大学环境与安全工程学院, 福州 350116;
3.
福州大学化学学院, 教育部食品安全与生物分析重点实验室, 福州 350116;
4.
闽江师范高等专科学校化学与生物技术学院, 福州 350108

通讯作者: 张兰,E-mail:zlan@fzu.edu.cn

基金项目:
国家自然科学基金项目（No.21605022）、福建省科技重大专项专题项目（No.2020YZ019007）和福州市科技计划项目（No.2019-S-66）资助。

摘要: 利用纳米金(AuNPs)可在对苯二酚作为弱还原剂条件下特异性催化银离子沉积的金标银染信号放大特性,制备了一种高效、灵敏检测冈田酸(OA)的电化学核酸适配体传感器,实现了复杂样品中痕量OA的检测。当溶液中存在OA时,电极表面的OA适配体可与溶液中的OA特异性结合而从电极表面解离下来,标记了AuNPs的信号探针再通过与游离的捕获探针杂交反应将AuNPs修饰至电极表面,然后将电极浸泡到银染试剂中进行银染反应,最后通过方波伏安法(SWV)直接检测纳米银的氧化信号,从而实现对OA的检测。在最佳实验条件下,此传感器的线性范围为10.0 pg/mL~500.0 ng/mL,检出限(3σ/k)为8.8 pg/mL。将此传感器应用于淡菜样品中OA的检测,测得其可食用部分中OA的含量为36.0 ng/g,低于欧盟的限量标准(160.0 ng/g),加标回收率为94.2%~112.0%。

关键词:

English

An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label

1.
Fujian College Association Instrument Analysis Center, Fuzhou University, Fuzhou 350116, China;
2.
College of Environment and Safety Engineering, Fuzhou University, Fuzhou 350116, China;
3.
Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education, College of Chemistry, Fuzhou University, Fuzhou 350116, China;
4.
School of Chemical and Biological Technology, Minjiang Teachers College, Fuzhou 350108, China

Corresponding author: ZHANG Lan, zlan@fzu.edu.cn

Received Date: 17 April 2022
Revised Date: 13 June 2022
Fund Project:
Supported by the National Nature Science Foundation of China (No.21605022), the Special Fund for Key Program of Science and Technology of Fujian Province, China (No.2020YZ019007) and the Fuzhou Science and Technology Plan Project, China (No.2019-S-66).

Abstract: Based on silver-enhanced gold nanoparticles (AuNPs) label technique that AuNPs could specifically catalyze the deposition of silver ions using hydroquin as a weak reducing agent, an efficient and sensitive electrochemical aptasensor was developed for detection of trace okadaic acid (OA). When the sample solution containing OA, the OA aptamer dissociated from the dsDNA on electrode surface due to the specific binding between OA and OA aptamer, AuNPs labeled signal probe (Sp) could hybridize with the resulted free capture probe (Cp) on the electrode surface. And then, the electrode was immersed in a silver staining reagent to perform following sliver staining. Finally, the oxidation signal of the nano-silver was directly detected by square wave voltammetry (SWV). Under the optimal experimental conditions, the linear range of the established OA aptasensor was 10.0 pg/mL-500.0 ng/mL, and the detection limit (3σ/k) was 8.8 pg/mL. The sensor was applied to detection of mussels. The experimental results showed that the content of OA in the edible part of mussels was 36.0 ng/g, which was lower than the permitted maximal limit of European Union (160.0 ng/g), and the recoveries were 94.2%-112.0%.

Key words:

1. [1]
  NAI H, CAO L, SUN S, LI R, LIU H, YAN P. Ionics, 2021, 27(12):5323-5331.NAI H, CAO L, SUN S, LI R, LIU H, YAN P. Ionics, 2021, 27(12):5323-5331.
2. [2]
  CHEN Jia-Qi, WU Hai-Yan, YAO Lin, ZHENG Guan-Chao, GUO Meng-Meng, TAN Zhi-Jun, ZHAI Yu-Xiu, MU Hai-Jin. Chin. J. Anal. Chem., 2018, 46(8):1261-1268. 陈佳琦, 吴海燕, 姚琳, 郑关超, 郭萌萌, 谭志军, 翟毓秀, 牟海津. 分析化学, 2018, 46(8):1261-1268.
3. [3]
  FANG Li-Yuan, LI Dai-Zong, XIAO Qin. Chin. Fishery Quality Standards, 2017, 7(1):41-49. 方丽媛, 李代宗, 肖勤. 中国渔业质量与标准, 2017, 7(1):41-49.
4. [4]
  LIANG Y, LI A, CHEN J, TAN Z, TONG M, LIU Z, QIU J, YU R. Harmful Algae, 2022, 111:102152.LIANG Y, LI A, CHEN J, TAN Z, TONG M, LIU Z, QIU J, YU R. Harmful Algae, 2022, 111:102152.
5. [5]
  European Food Safety Authority[WWW Document], 2008. https://www.efsa.europa.eu/en/press/news/080131(accessed 1.4.19).European Food Safety Authority[WWW Document], 2008. https://www.efsa.europa.eu/en/press/news/080131(accessed 1.4.19).
6. [6]
  LOUPPIS A P, BADEKA A V, KATIKOU P, PALEOLOGOS E K, KONTOMINAS M G. Toxicon, 2010, 55:724-733.LOUPPIS A P, BADEKA A V, KATIKOU P, PALEOLOGOS E K, KONTOMINAS M G. Toxicon, 2010, 55:724-733.
7. [7]
  CHEN J Q, TAN Z J, WU H Y, PENG J X, ZHAI Y X, GUO M M. J. Sep. Sci., 2019, 42(7):1423-1431.CHEN J Q, TAN Z J, WU H Y, PENG J X, ZHAI Y X, GUO M M. J. Sep. Sci., 2019, 42(7):1423-1431.
8. [8]
  WANG L, SHI X Z, ZHAO Q L, SUN A L, LI D X, ZHAO J. Food Chem., 2019, 272:427-433.WANG L, SHI X Z, ZHAO Q L, SUN A L, LI D X, ZHAO J. Food Chem., 2019, 272:427-433.
9. [9]
  BOSCH-OREA C, SANCHÍS J, FARRÉ M, BARCELÓ D. Anal. Bioanal. Chem., 2017, 409(23):5451-5462.BOSCH-OREA C, SANCHÍS J, FARRÉ M, BARCELÓ D. Anal. Bioanal. Chem., 2017, 409(23):5451-5462.
10. [10]
  YUAN Tao, LIU Zhong-Yuan, HU Lian-Zhe, XU Guo-Bao. Chin. J. Anal. Chem., 2011, 39(7):972-977. 袁涛, 刘中原, 胡连哲, 徐国宝. 分析化学, 2011, 39(7):972-977.
11. [11]
  LI Hai-Yu, LI Wen-Ya, WANG Wan, LYU Qing, WANG Zhi-Juan, ZHANG Qing. Chin. J. Anal. Chem., 2021, 49(3):415-423. 李海玉, 李文雅, 王婉, 吕庆, 王志娟, 张庆. 分析化学, 2021, 49(3):415-423.
12. [12]
  YUAN Min, QIAN Shi-Quan, CAO Hui, XU Fei, YE Tai,YU Jin-Song, GUO Wen, WU Jia-Ying, A TI-kAN·Wu-Siman. Chin. J. Anal. Chem., 2020, 48(12):1701-1708. 袁敏, 钱世权, 曹慧, 徐斐, 叶泰,于劲松, 郭文, 吴嘉颖, 阿提坎·吾斯曼. 分析化学, 2020, 48(12):1701-1708.
13. [13]
  EISSA S, NG A, SIAJ M, TAVARES A C, ZOUROB M. Anal. Chem., 2013, 85(24):11794-11801.EISSA S, NG A, SIAJ M, TAVARES A C, ZOUROB M. Anal. Chem., 2013, 85(24):11794-11801.
14. [14]
  CHEN Jia-Qi, WU Hai-Yan, ZHANG Xu-Zhi, ZHENG Guan-Chao, SUN Xiao-Jie, GUO Meng-Meng, TAN Zhi-Jun, ZHAI Yu-Xiu, MU Hai-Jin. Chin. J. Anal. Chem., 2019, 47(6):869-875. 陈佳琦, 吴海燕, 张旭志, 郑关超, 孙晓杰, 郭萌萌, 谭志军, 翟毓秀, 牟海津. 分析化学, 2019, 47(6):869-875.
15. [15]
  TATON T A, MIRKIN C A, LETSINGER R L. Science, 2000, 289(5485):1757-1760.TATON T A, MIRKIN C A, LETSINGER R L. Science, 2000, 289(5485):1757-1760.
16. [16]
  PARK S J, TATON T A, MIRKIN C A. Science, 2002, 295(5559):1503-1506.PARK S J, TATON T A, MIRKIN C A. Science, 2002, 295(5559):1503-1506.
17. [17]
  LAI G S, WU J, JU H X, YAN F. Adv. Funct. Mater., 2011, 21(15):2938-2943.LAI G S, WU J, JU H X, YAN F. Adv. Funct. Mater., 2011, 21(15):2938-2943.
18. [18]
  TONG L, WU J, LI J, JU H X, YAN F. Analyst, 2013, 138(17):4870-4876.TONG L, WU J, LI J, JU H X, YAN F. Analyst, 2013, 138(17):4870-4876.
19. [19]
  LIN D J, WU J, JU H X, YAN F. Biosens. Bioelectron., 2014, 52:153-158.LIN D J, WU J, JU H X, YAN F. Biosens. Bioelectron., 2014, 52:153-158.
20. [20]
  LIU J, LU Y. Nat. Protoc., 2006, 1(1):246-252.LIU J, LU Y. Nat. Protoc., 2006, 1(1):246-252.
21. [21]
  HAISS W, THANH N T K, AVEYARD J, FERNIG D G. Anal. Chem., 2007, 79(11):4215-4221.HAISS W, THANH N T K, AVEYARD J, FERNIG D G. Anal. Chem., 2007, 79(11):4215-4221.
22. [22]
  WU Lei, LYU Tao-Lin, CHEN Qi-Zhong, LIU Yong-Hui, XIE Jing-Ying. Chin. J. Power Sources, 2021, 45(9):1227-1230. 吴磊, 吕桃林, 陈启忠, 刘咏晖, 解晶莹. 电源技术, 2021, 45(9):1227-1230.
23. [23]
  TING B P, ZHANG J, KHAN M, YANG Y Y, YING J Y. Chem. Commun., 2009, 41:6231-6233.TING B P, ZHANG J, KHAN M, YANG Y Y, YING J Y. Chem. Commun., 2009, 41:6231-6233.

扫一扫看文章

计量

PDF下载量: 15
文章访问数: 626
HTML全文浏览量: 51

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于金标银染技术的冈田酸核酸适配体传感器的研究

通讯作者: 张兰,E-mail:zlan@fzu.edu.cn

English

An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label

Corresponding author: ZHANG Lan, zlan@fzu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于金标银染技术的冈田酸核酸适配体传感器的研究

通讯作者: 张兰,E-mail:zlan@fzu.edu.cn

English

An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label

Corresponding author: ZHANG Lan, zlan@fzu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs