Citation: TONG Ping, ZHANG Hui-Ling, LU Wei, ZHANG Wen-Min, ZHANG Lan. An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(9): 1355-1363. doi: 10.19756/j.issn.0253-3820.221191 shu

An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label

TONG Ping ^1,2,3 ,
ZHANG Hui-Ling ^1,2 ,
LU Wei ³ ,
ZHANG Wen-Min ^3,4 ,
ZHANG Lan ^3,,

1.
Fujian College Association Instrument Analysis Center, Fuzhou University, Fuzhou 350116, China;
2.
College of Environment and Safety Engineering, Fuzhou University, Fuzhou 350116, China;
3.
Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education, College of Chemistry, Fuzhou University, Fuzhou 350116, China;
4.
School of Chemical and Biological Technology, Minjiang Teachers College, Fuzhou 350108, China

Corresponding author: ZHANG Lan, zlan@fzu.edu.cn
Received Date: 17 April 2022
Revised Date: 13 June 2022

Fund Project: Supported by the National Nature Science Foundation of China (No.21605022), the Special Fund for Key Program of Science and Technology of Fujian Province, China (No.2020YZ019007) and the Fuzhou Science and Technology Plan Project, China (No.2019-S-66).

Based on silver-enhanced gold nanoparticles (AuNPs) label technique that AuNPs could specifically catalyze the deposition of silver ions using hydroquin as a weak reducing agent, an efficient and sensitive electrochemical aptasensor was developed for detection of trace okadaic acid (OA). When the sample solution containing OA, the OA aptamer dissociated from the dsDNA on electrode surface due to the specific binding between OA and OA aptamer, AuNPs labeled signal probe (Sp) could hybridize with the resulted free capture probe (Cp) on the electrode surface. And then, the electrode was immersed in a silver staining reagent to perform following sliver staining. Finally, the oxidation signal of the nano-silver was directly detected by square wave voltammetry (SWV). Under the optimal experimental conditions, the linear range of the established OA aptasensor was 10.0 pg/mL-500.0 ng/mL, and the detection limit (3σ/k) was 8.8 pg/mL. The sensor was applied to detection of mussels. The experimental results showed that the content of OA in the edible part of mussels was 36.0 ng/g, which was lower than the permitted maximal limit of European Union (160.0 ng/g), and the recoveries were 94.2%-112.0%.
- Silver-enhanced gold nanoparticle label,
- Okadaic acid,
- Aptasensor,
- Signal amplification
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