Citation: WANG Jun-Yang, DUAN Zi-Xuan, WU Tong, WANG Wei, SUN Chun-Yan. Visual and Quantitative Detection of Lead Ion Based on G-quadruplex-hemin DNAzyme-mediated Etching of Gold Nanorods[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1473-1481. doi: 10.19756/j.issn.0253-3820.221180 shu

Visual and Quantitative Detection of Lead Ion Based on G-quadruplex-hemin DNAzyme-mediated Etching of Gold Nanorods

College of Food Science and Engineering, Jilin University, Changchun 130062, China

Corresponding author: SUN Chun-Yan, sunchuny@jlu.edu.cn
Received Date: 13 April 2022
Revised Date: 2 July 2022

Fund Project: Supported by the Innovation Training Project of Jilin University's "College Student Innovation and Entrepreneurship Training Program" (No.202110183164).

A label-free visualized lead ion biosensor was constructed based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorods (AuNRs). Hemin could induce the transformation of the aptamer PS2.M into a G-quadruplex structure to generate a G-quadruplex-hemin DNAzyme with peroxidase-like activity. 3,3',5,5'-Tetramethylbenzidine (TMB) was oxidized to form a blue diimine derivative TMB⁺, and the enzymatic reaction was terminated after adding H₂SO₄, and TMB⁺ became TMB²⁺ (yellow). Under the action of hexadecyl trimethyl ammonium bromide (CTAB), TMB²⁺ could effectively oxidize Au(0) to Au(I), thereby realizing the etching of AuNRs, and the solution turned yellow. Due to the highly specific interaction between PS2.M and Pb²⁺, and the ability of Pb²⁺ to stabilize G-quadruplexes was significantly stronger than that of hemin, G-quadruplexes-hemin DNAzyme with enzyme-like activity could not be formed in the presence of Pb²⁺, thus unable to etch AuNRs. Therefore, different concentrations of Pb²⁺ made the solutions produce distinct color changes that could be easily identified with naked eyes. The ratiometric quantitative detection of Pb²⁺ was achieved by measuring the absorbance of the longitudinal surface plasmon absorption peak of AuNRs and the TMB⁺ absorption peak at 450 nm, with a linear range of 5-5000 nmol/L and a detection limit as low as 2.0 nmol/L. The method was successfully applied to detection of Pb²⁺ in Songhua River water and tap water samples, with spiked recoveries were 98.7%-102.5% and 94.8%-108.2%, respectively, and the results were consistent with the detection results of inductively coupled plasma-mass spectrometry (ICP-MS). The method ingeniously designed the nucleic acid sequence, and realized the rapid and high-sensitivity visual detection of Pb²⁺.
- Aptamer,
- G-quadruplex-hemin DNAzyme,
- Lead ion,
- Gold nanorods,
- Visualization
1. [1]
2. [2]
  BI X Y, LI Z G, WANG S X, ZHANG L, XU R, LIU J L, YANG H M, GUO M Z. Environ. Sci. Technol., 2017, 51(22):13502-13508.
3. [3]
  LLOBET J M, FALCO G, CASAS C, TEIXIDO A, DOMINGO J L. J.Agric. Food Chem., 2003, 51(3):838-842.
4. [4]
  HSU P C, GUO Y L. Toxicology, 2002, 180(1):33-44.
5. [5]
  World Health Organization (2017) Guidelines for Drinking Water Quality. WHO, Geneva.
6. [6]
  MA R, VAN MOL W, ADAMS F. Anal. Chim. Acta, 1994, 293(3):251-260.
7. [7]
  WAN Z, XU Z, WANG J. Analyst, 2006, 131(1):141-147.
8. [8]
  COCHERIE A, ROBERT M. Chem. Geol., 2007, 243(1-2):90-104.
9. [9]
  ELFERING H, ANDERSSON J, POLL K. Analyst, 1998, 123(4):669-674.
10. [10]
  OCHSENKUHN-PETROPOULOU M, OCHSENKUHN K M, Fresenius' J. Anal. Chem., 2001, 369(7-8):629-632.
11. [11]
  MA Y D, YU C, YU Y, CHEN J, GAO R F, HE J L. Microchim. Acta, 2019, 186(10):677.
12. [12]
  POOLSUP S, KIM C Y. Curr. Opin. Biotechnol., 2017, 48:180-186.
13. [13]
  ZHANG Y, LAI B S, JUHAS M. Molecules, 2019, 24(5):941.
14. [14]
  SCHLUP T, COONEY C. Nucleic Acids Res., 1998, 26(19):4524-4530.
15. [15]
  WANG R E, ZHANG Y, CAI J F, CAI W B, GAO T. Curr. Med. Chem., 2011, 18(27):4175-4184.
16. [16]
  ZHOU W, DING J, LIU J. Theranostics, 2017, 7(4):1010-1025.
17. [17]
  PENG H, NEWBIGGING A M, WANG Z, TAO J, DENG W, LE X C, ZHANG H. Anal. Chem., 2018, 90(1):190-207.
18. [18]
  LI Y, SEN D. Nat. Struct. Biol., 1996, 3(9):743-747.
19. [19]
  LI Y, SEN D. Biochemistry, 1997, 36(18):5589-5599.
20. [20]
  LI Y, SEN D. Chem. Biol., 1998, 5(1):1-12.
21. [21]
  TRAVASCIO P, LI Y, SEN D. Chem. Biol., 1998, 5(9):505-517.
22. [22]
  ZHANG D, WANG W, DONG Q, HUANG Y, WEN D, MU Y, YUAN Y. Microchim. Acta, 2018, 185(1):75.
23. [23]
  SANG Y, XU Y J, XU L L, CHENG W, LI X M, WU J L. Microchim. Acta, 2017, 184(7):2465-2471.
24. [24]
  NASIR M, NAWAZ M H, LATIF U, YAQUB M, HAYAT A, RAHIM A. Microchim. Acta, 2017, 184(2):323-342.
25. [25]
  ZONG C, ZHANG D, YANG H, WANG S, CHU M, LI P. Microchim. Acta, 2017, 184(9):3197-3204.
26. [26]
  HUANG X, EL-SAYED I H, QIAN W, EL-SAYED M A. J. Am. Chem. Soc, 2006, 128(6):2115-2120.
27. [27]
  TSUNG C K, KOU X, SHI Q, ZHANG J, YEUNG M H, WANG J, STUCKY G D. J. Am. Chem. Soc., 2006, 128(16):5352-5353.
28. [28]
  NIKOOBAKHT B, EL-SAYED M A. Chem. Mater., 2003, 15(10):1957-1962.
29. [29]
  CHEN G Z, BAI W S, JIN Y, ZHENG J B. Talanta, 2021, 232:122405.
30. [30]
  SANNIGRAHI A, CHOWDHURY S, NANDI I, SANYAL D, CHALL S, CHATTOPADHYAY K. Nanoscale Adv., 2019, 1(9):3660-3669.
31. [31]
32. [32]
  YUAN M, SONG Z H, FEI J Y, WANG X L, XU F, CAO H, YU J S. Microchim. Acta, 2017, 184(5):1397-1403.
33. [33]
  CHEN Y, LI H H, GAO T, ZHANG T T, XU L J, WANG B, WANG J N, PEI R J. Sens. Actuators, B, 2018, 254:214-221.
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