新型聚多巴胺/聚丙烯酸-氢氧化铜双面神纳米粒子的制备及在体外光声影像和化疗-热疗协同癌症治疗中的应用

引用本文: 曹晴, 李东, 姜艳霞, 戚克振, 张满杰. 新型聚多巴胺/聚丙烯酸-氢氧化铜双面神纳米粒子的制备及在体外光声影像和化疗-热疗协同癌症治疗中的应用[J]. 分析化学, 2022, 50(8): 1205-1216. doi: 10.19756/j.issn.0253-3820.221122 shu

Citation: CAO Qing, LI Dong, JIANG Yan-Xia, QI Ke-Zhen, ZHANG Man-Jie. Fabrication of Unique Polydopamine/Poly(acrylic acid)-Copper Hydroxide Janus Nanoparticles for Photoacoustic Imaging and Chemo-Photothermal Cancer Therapy in Vitro[J]. Chinese Journal of Analytical Chemistry, 2022, 50(8): 1205-1216. doi: 10.19756/j.issn.0253-3820.221122 shu

新型聚多巴胺/聚丙烯酸-氢氧化铜双面神纳米粒子的制备及在体外光声影像和化疗-热疗协同癌症治疗中的应用

曹晴 ^1, ,
李东 ^1, ,
姜艳霞 ^1, ,
戚克振 ^{2,
,} ,
张满杰 ^{1,
,}

1.
沈阳师范大学化学化工学院, 能源与环境催化研究所, 沈阳 110034;
2.
大理大学药学院, 大理 671000

通讯作者: 戚克振,E-mail:qkzhen2003@aliyun.com; 张满杰,E-mail:zhangmj454@nenu.edu.cn

基金项目:
国家自然科学基金项目(No.22002096)、辽宁省自然科学基金项目(No.2019-BS-219)和辽宁省兴辽英才计划(No.XLYC1807238)资助。 # 共同第一作者

摘要: 制备了双面神(Janus)结构的聚多巴胺/聚丙烯酸-氢氧化铜纳米粒子(PDA/PAA-Cu (OH)₂ JNPs)。PDA在近红外(NIR)区有较强的吸收,并且具有优异的生物相容性和可降解性;PAA纳米球与铜离子(Cu²⁺)配位后形成的PAA-Cu (OH)₂纳米粒子具有介孔结构,可用于装载抗癌药物;Cu (OH)₂在NIR区有较强的吸收,可用于光热治疗,实现不同功能有机融合,展现协同增效。选用亲水的阿霉素(DOX)作为药物模型,研究了此药物递送系统对肿瘤细胞(HepG-2)的抑制效果。合成的双面神纳米粒子具有高的药物(阿霉素)装载能力(药物负载容量=0.87 mg/mg)、良好的光热转换效率(45.9%)、pH/近红外(NIR)双重响应药物释放性质和光声(PA)成像能力,可用于体外PA影像和化疗-热疗协同癌症治疗。体外毒性实验结果表明,DOX负载的PDA/PAA-Cu (OH)₂ JNPs加激光组呈现明显的癌细胞死亡,细胞存活率极低(7.9%)。

关键词:

English

Fabrication of Unique Polydopamine/Poly(acrylic acid)-Copper Hydroxide Janus Nanoparticles for Photoacoustic Imaging and Chemo-Photothermal Cancer Therapy in Vitro

CAO Qing ^1, ,
LI Dong ^1, ,
JIANG Yan-Xia ^1, ,
QI Ke-Zhen ^{2,
,} ,
ZHANG Man-Jie ^{1,
,}

1.
Institute of Catalysis for Energy and Environment, College of Chemistry and Chemical Engineering, Shenyang Normal University, Shenyang 110034, China;
2.
Institute of Pharmacy, Dali University, Dali 671000, China

Corresponding author: QI Ke-Zhen, ; ZHANG Man-Jie,

Received Date: 11 March 2022
Revised Date: 27 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.22002096), the Natural Science Foundation of Liaoning Province, China (No.2019BS219) and the Liaoning Revitalization Talents Program (No.XLYC1807238).

Abstract: The polydopamine/poly(acrylic acid)-copper hydroxide Janus nanoparticles (PDA/PAA-Cu(OH)₂ JNPs) were prepared by a novel and simple method. PDA had a strong absorption in the near infrared (NIR) region, with excellent biocompatibility and degradation, and the PAA-Cu(OH)₂ was formed via coordination between the carboxyl groups of PAA and copper ions (Cu²⁺) by introducing Cu(NO₃)₂, which was used for loading anticancer drug. The PAA-Cu(OH)₂ domains also had strong absorption in the NIR region for photothermal therapy, realizing the organic fusion of different functions and showing synergistic effect. The hydrophilic doxorubicin (DOX) was chosen as drug model for exposing the inhibition efficiency of the HepG-2 cells. The obtained JNPs had high drug-loading contents (0.87 mg/mg, DOX/JNPs), good photothermal conversion efficiency (45.9%), pH/NIR bimodal-triggered controlled drug release ability and photoacoustic (PA) imaging capacity, enabling the JNPs to be applied to PA imaging and synergistic cancer chemo-phototherapy in vitro. The cytotoxicity assay in vitro proved that the group treated with DOX-loaded PDA/PAA-Cu(OH)₂ JNPs plus laser showed distinct cell death, and the viability of the cells were exceedingly low (7.9%).

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

新型聚多巴胺/聚丙烯酸-氢氧化铜双面神纳米粒子的制备及在体外光声影像和化疗-热疗协同癌症治疗中的应用

通讯作者: 戚克振,E-mail:qkzhen2003@aliyun.com; 张满杰,E-mail:zhangmj454@nenu.edu.cn

English

Fabrication of Unique Polydopamine/Poly(acrylic acid)-Copper Hydroxide Janus Nanoparticles for Photoacoustic Imaging and Chemo-Photothermal Cancer Therapy in Vitro

Corresponding author: QI Ke-Zhen, ; ZHANG Man-Jie,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

新型聚多巴胺/聚丙烯酸-氢氧化铜双面神纳米粒子的制备及在体外光声影像和化疗-热疗协同癌症治疗中的应用

通讯作者: 戚克振,E-mail:qkzhen2003@aliyun.com; 张满杰,E-mail:zhangmj454@nenu.edu.cn

English

Fabrication of Unique Polydopamine/Poly(acrylic acid)-Copper Hydroxide Janus Nanoparticles for Photoacoustic Imaging and Chemo-Photothermal Cancer Therapy in Vitro

Corresponding author: QI Ke-Zhen, ; ZHANG Man-Jie,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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