基于DNA折纸的酶级联催化用于低密度脂蛋白的电化学检测

余紫荆 肖明书 裴昊 李丽

引用本文: 余紫荆, 肖明书, 裴昊, 李丽. 基于DNA折纸的酶级联催化用于低密度脂蛋白的电化学检测[J]. 分析化学, 2022, 50(6): 850-858. doi: 10.19756/j.issn.0253-3820.221111 shu
Citation:  YU Zi-Jing,  XIAO Ming-Shu,  PEI Hao,  LI Li. DNA Origami-based Enzyme Cascade Catalysis for Electrochemical Detection of Low Density Lipoprotein[J]. Chinese Journal of Analytical Chemistry, 2022, 50(6): 850-858. doi: 10.19756/j.issn.0253-3820.221111 shu

基于DNA折纸的酶级联催化用于低密度脂蛋白的电化学检测

    通讯作者: 李丽,E-mail:lli@chem.ecnu.edu.cn
  • 基金项目:

    国家自然科学基金项目(Nos.22174046,22104038)、上海市科学技术委员会(Nos.22ZR1419800,18490740500)和中国博士后科学基金项目(Nos.2020TQ0097,2021M701212)资助。

摘要: 很多疾病(如动脉粥样硬化)的发生与低密度脂蛋白(LDL)水平的上升密切相关,发展快速、灵敏的LDL分析方法对于相关疾病的早期诊断具有重要意义。本研究构建了一种基于DNA折纸的酶级联催化电化学传感器用于LDL的检测。基于核酸的可编程性和可寻址性,可将胆固醇氧化酶和辣根过氧化物酶特异性锚定在DNA折纸上,并通过控制酶间距(如20、40和80nm),实现了对LDL级联催化效率的优化。通过Au—S化学键,将负载酶的DNA折纸结构修饰在金电极表面,构建了酶级联催化电化学传感器。此传感器对LDL检测具有优异性能,在2~15μmol/L浓度范围内具有良好的线性关系,并且检出限低(1.8μmol/L (S/N=3)),选择性良好。将此传感器应用于健康人血清中LDL的检测,加标回收率在97.8%~103.6%之间,表明此传感器具有良好的重现性、稳定性和实用性。基于DNA折纸的酶级联催化电化学传感器为LDL的快速、灵敏检测提供了一种新方法,有望用于动脉粥样硬化的早期诊断。

English


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  • 收稿日期:  2022-03-01
  • 修回日期:  2022-03-21
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