集成DNA分子机器的微针贴片用于细胞外三磷酸腺苷原位监测

引用本文: 闫璐, 李晓丹, 肖明书, 裴昊. 集成DNA分子机器的微针贴片用于细胞外三磷酸腺苷原位监测[J]. 分析化学, 2022, 50(4): 516-524. doi: 10.19756/j.issn.0253-3820.221101 shu

Citation: YAN Lu, LI Xiao-dan, XIAO Ming-shu, PEI Hao. DNA Molecular Machine-Integrated Microneedle Patches for in Situ Monitoring of Extracellular Adenosine Triphosphate[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 516-524. doi: 10.19756/j.issn.0253-3820.221101 shu

集成DNA分子机器的微针贴片用于细胞外三磷酸腺苷原位监测

闫璐 ^1,2, ,
李晓丹 ^1,2, ,
肖明书 ^1,2, ,
裴昊 ^{1,2,
,}

1.
华东师范大学化学与分子工程学院, 上海市绿色化学与化学过程重点实验室, 上海 200241;2 崇明生态研究院, 上海 202162

通讯作者: 裴昊,E-mail:peihao@chem.ecnu.edu.cn

基金项目:
中国博士后科学基金项目(Nos.2020TQ0097,2021M701212)资助

国家自然科学基金项目(Nos.22074041,22104038)

摘要: 细胞外三磷酸腺苷(ATP)作为信号分子参与一系列生理过程,其代谢异常与很多疾病(如炎症和感染性疾病等)的发生密切相关。发展胞外ATP的原位检测方法对于阐明相关疾病的发病机理及临床诊断具有重要意义。本研究制备了集成DNA分子机器的微针贴片。此微针贴片具有快速溶胀特性,在水溶液中可实现对ATP原位采样;集成的DNA分子机器在ATP驱动下行走,可实现荧光信号放大。将此微针贴片分析系统用于ATP传感,具有宽的检测范围(1~500μmol/L)、低检出限(1μmol/L)和良好的选择性。将此微针贴片分析系统直接用于原位监测0.1 mol/L K⁺刺激下活细胞分泌的ATP,测得浓度为(10.9±1.6)μmol/L,与相关文献报道结果一致。本研究为细胞外ATP原位监测提供了一个有效的分析平台。

关键词:

English

DNA Molecular Machine-Integrated Microneedle Patches for in Situ Monitoring of Extracellular Adenosine Triphosphate

1.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China;2 Institute of Eco-Chongming, Shanghai 202162, China

Corresponding author: PEI Hao, peihao@chem.ecnu.edu.cn

Received Date: 25 February 2022
Revised Date: 17 March 2022
Fund Project:
the China Postdoctoral Science Foundation(Nos. 2020TQ0097, 2021M701212)

Supported by the National Natural Science Foundation of China(Nos. 22074041, 22104038)

Abstract: Extracellular adenosine triphosphate(ATP) as a signaling molecule participates in a series of physiological processes, and its abnormal metabolism is closely related to many diseases(e.g., inflammation, infectious diseases). Developing methods for in situ detection of extracellular ATP is of great importance to elucidating the mechanism of relevant diseases and their diagnosis. This work reports DNA molecular machine-integrated microneedle patches. The developed microneedle patch allows for rapidly swelling in aqueous solution and in-situ sampling of target ATP molecules; the DNA molecular machine driven by ATP can realize fluorescence signal amplification. Benefiting from these traits, the microneedle patch-based analytical system shows excellent performance for ATP detection, including wide detection range(1-500 μmol/L), low limit of detection(1 μmol/L) and good selectivity.The result demonstrated that this microneedle patch-based analytical system enables in situ monitoring of ATP secreted by living cells under the stimulation of 0.1 mol/L K⁺, and the ATP concentration is determined to be(10.9 ± 1.6) μmol/L, consistent with previous work. This work provides a promising analytical platform for in situ monitoring of extracellular ATP.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

集成DNA分子机器的微针贴片用于细胞外三磷酸腺苷原位监测

通讯作者: 裴昊,E-mail:peihao@chem.ecnu.edu.cn

English

DNA Molecular Machine-Integrated Microneedle Patches for in Situ Monitoring of Extracellular Adenosine Triphosphate

Corresponding author: PEI Hao, peihao@chem.ecnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

集成DNA分子机器的微针贴片用于细胞外三磷酸腺苷原位监测

通讯作者: 裴昊,E-mail:peihao@chem.ecnu.edu.cn

English

DNA Molecular Machine-Integrated Microneedle Patches for in Situ Monitoring of Extracellular Adenosine Triphosphate

Corresponding author: PEI Hao, peihao@chem.ecnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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