一步法制备高导电聚3,4-乙烯二氧噻吩∶聚苯乙烯磺酸水凝胶及其电化学传感研究

引用本文: 崔入文, 冯涛涛, 高楠, 张美宁. 一步法制备高导电聚3,4-乙烯二氧噻吩∶聚苯乙烯磺酸水凝胶及其电化学传感研究[J]. 分析化学, 2022, 50(6): 859-868. doi: 10.19756/j.issn.0253-3820.221091 shu

Citation: CUI Ru-wen, FENG Tao-tao, GAO Nan, ZHANG Mei-ning. Preparation of Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrene sulfonate) Hydrogel by One-step Method for Electrochemical Sensor[J]. Chinese Journal of Analytical Chemistry, 2022, 50(6): 859-868. doi: 10.19756/j.issn.0253-3820.221091 shu

一步法制备高导电聚3,4-乙烯二氧噻吩∶聚苯乙烯磺酸水凝胶及其电化学传感研究

中国人民大学化学系, 北京 100872

通讯作者: 张美宁,E-mail:mnzhang@ruc.edu.cn

基金项目:
国家自然科学基金项目（Nos.21874152，22174162）资助。

摘要: 作为一种新型导电聚合物水凝胶材料，聚3，4-乙烯二氧噻吩∶聚苯乙烯磺酸（PEDOT∶PSS）在生物电子和生物传感领域受到越来越多的关注。然而，目前PEDOT∶PSS水凝胶的制备大多需要以非导电水凝胶为模板，或制备条件苛刻，这极大地影响了其在电化学生物传感领域的应用。本研究建立了一种简单的制备PEDOT∶PSS水凝胶的新方法，将含有乙二醇的PEDOT∶PSS水溶液经过真空干燥处理，再次吸水溶胀后即可获得稳定、具有三维交联网络的PEDOT∶PSS水凝胶。制备的PEDOT∶PSS水凝胶具有微/纳多级孔及三维导电网络结构，电导率可达到（3321.33±129.41） S/m，形貌可稳定保持2个月。PEDOT∶PSS水凝胶修饰的电极不但具有良好的异相电子转移和传质能力，而且可在一个月内有效抵抗蛋白质吸附对电化学信号的影响。水凝胶的三维网络可稳定地固载酶，并显著提高了酶电化学传感器的稳定性，酶传感器在储存1个月后的信号强度仍保持在初始信号强度的90%以上。此水凝胶为电化学传感器检测复杂生物样品提供了新的策略。

关键词:

English

Preparation of Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrene sulfonate) Hydrogel by One-step Method for Electrochemical Sensor

Department of Chemistry, Renmin University of China, Beijing 100872, China

Corresponding author: ZHANG Mei-ning, mnzhang@ruc.edu.cn

Received Date: 22 February 2022
Revised Date: 25 March 2022
Fund Project:
Supported by the National Natural Science Foundation of China(Nos. 21874152, 22174162).

Abstract: Conducting polymers-based hydrogels, especially poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), have received increasing attention in the fields of bioelectronics and biosensors. However, current preparation methods of PEDOT:PSS hydrogel mostly use non-conductive hydrogel as a pre-template or severe treatment condition, which greatly limits the electrochemical biosensing application of hydrogels. Herein, we report a novel method for preparing PEDOT:PSS hydrogel. The PEDOT:PSS aqueous solution containing ethylene glycol is treated by vacuum drying,then the stable PEDOT:PSS hydrogel with three-dimensional cross-linking network is obtained by swelling. The prepared PEDOT:PSS hydrogel with micro/nano hierarchical pores and three-dimensional conductive network structure, has high conductivity(3321.33 ±129.41 S/m) and high stability in 2 months.The hydrogel-modified electrode not only has good property of heterogeneous electron transfer and mass transfer ability, but also can effectively resist non-specific adsorption of protein more than 1 month.The PEDOT:PSS hydrogel can stably entrap enzyme and maintain its activity, and the initial signal of the enzyme can be maintained more than 90% after 1 month. Therefore, the hydrogel provides a new strategy for constructing electrochemical sensors to detect interested substances in complex biological samples.

Key words:

Poly(3
/ 4-ethylenedioxythiophene):poly(styrene sulfonate)
/ Conducting polymer hydrogel
/ Vacuum drying
/ Electrochemical biosensor
/ Anti-protein adsorption

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沈阳化工大学材料科学与工程学院沈阳 110142

一步法制备高导电聚3,4-乙烯二氧噻吩∶聚苯乙烯磺酸水凝胶及其电化学传感研究

通讯作者: 张美宁,E-mail:mnzhang@ruc.edu.cn

English

Preparation of Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrene sulfonate) Hydrogel by One-step Method for Electrochemical Sensor

Corresponding author: ZHANG Mei-ning, mnzhang@ruc.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

一步法制备高导电聚3,4-乙烯二氧噻吩∶聚苯乙烯磺酸水凝胶及其电化学传感研究

通讯作者: 张美宁,E-mail:mnzhang@ruc.edu.cn

English

Preparation of Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrene sulfonate) Hydrogel by One-step Method for Electrochemical Sensor

Corresponding author: ZHANG Mei-ning, mnzhang@ruc.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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