利用重氮化反应构建比率型电分析方法测定亚硝酸盐

引用本文: 牛湘衡, 汪梦珠, 胡盼旺, 刘邦祥. 利用重氮化反应构建比率型电分析方法测定亚硝酸盐[J]. 分析化学, 2022, 50(6): 869-877. doi: 10.19756/j.issn.0253-3820.221083 shu

Citation: NIU Xiang-Heng, WANG Meng-Zhu, HU Pan-Wang, LIU Bang-Xiang. A Ratiometric Electroanalytical Method Based on Diazotization Reaction for Detection of Nitrite[J]. Chinese Journal of Analytical Chemistry, 2022, 50(6): 869-877. doi: 10.19756/j.issn.0253-3820.221083 shu

利用重氮化反应构建比率型电分析方法测定亚硝酸盐

牛湘衡 ^{1,2,
,} ,
汪梦珠 ^2, ,
胡盼旺 ^2, ,
刘邦祥 ^2,

1.
安徽师范大学功能分子固体教育部重点实验室, 芜湖 241002;
2.
江苏大学绿色化学与化工技术研究院, 镇江 212013

通讯作者: 牛湘衡,E-mail:niuxiangheng@ujs.edu.cn

基金项目:
功能分子固体教育部重点实验室开放课题项目（No.FMS202001）和国家自然科学基金项目（No.21605061）资助。

摘要: 食品和水中过量的亚硝酸盐（Nitrite）会对人体健康和环境造成诸多危害，因此开发可靠、便捷、低成本的亚硝酸盐检测方法十分重要。本研究采用邻苯二胺（o-Phenylenediamine，OPD）作为探针，基于重氮化反应开发了一种便捷的比率型电分析方法，利用未修饰的丝网印刷电极（Screen-printed electrode，SPE）实现了对亚硝酸盐的高性能检测。OPD探针可在较低电位处产生一个氧化信号，亚硝酸盐可在较高电位处产生另一个氧化信号。当OPD与亚硝酸盐共存时，两者在酸性环境中会发生重氮化反应。随着亚硝酸盐浓度增大，其自身氧化信号增强，而游离的OPD因重氮化反应消耗而减少，导致此探针的氧化信号减弱，基于此实现了比率型电化学测量。本方法对亚硝酸盐的线性检测范围为10~300μmol/L，检出限（S/N=3）为4.7μmol/L，可对环境水样和腌酸水中的目标物进行准确测定。本方法的灵敏度和准确性优良，抗干扰能力强，操作简便，成本低廉，具有广阔的应用前景。

关键词:

English

A Ratiometric Electroanalytical Method Based on Diazotization Reaction for Detection of Nitrite

1.
Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Normal University, Wuhu 241002, China;
2.
Institute of Green Chemistry and Chemical Technology, Jiangsu University, Zhenjiang 212013, China

Corresponding author: NIU Xiang-Heng, niuxiangheng@ujs.edu.cn

Received Date: 20 February 2022
Revised Date: 03 March 2022
Fund Project:
Supported by the Key Laboratory of Functional Molecular Solids, Ministry of Education(No. FMS202001) and the National Natural Science Foundation of China(No. 21605061).

Abstract: Excessive nitrite in food and water can cause many hazards to human health and the environment, therefore, it is very important to develop reliable, convenient and low-cost methods for nitrite detection. In this study, o-phenylenediamine(OPD) was used as a probe to develop a convenient ratiometric electrochemical method to realize high-performance detection of nitrite on bare screen-printed electrodes. The OPD probe could produce an oxidation signal at a low potential, and nitrite could produce another oxidation signal at a high potential. When OPD coexisted with nitrite, a diazotization reaction occurred in the acidic environment. With the increase of nitrite, free OPD decreased due to reaction consumption, resulting in the decreasing oxidation signal of the probe, while the oxidation signal of nitrite was gradually increased. Based on this principle, ratiometric electrochemical measurement of nitrite was achieved with a linear range of 10-300 μmol/L and a detection limit of 4.7 μmol/L. Accurate determination of the target in environmental water and pickle water samples was also demonstrated. The ratiometric electrochemical method with excellent sensitivity and accuracy, strong anti-interference ability, simple operation and low cost showed broad application prospects.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

利用重氮化反应构建比率型电分析方法测定亚硝酸盐

通讯作者: 牛湘衡,E-mail:niuxiangheng@ujs.edu.cn

English

A Ratiometric Electroanalytical Method Based on Diazotization Reaction for Detection of Nitrite

Corresponding author: NIU Xiang-Heng, niuxiangheng@ujs.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

利用重氮化反应构建比率型电分析方法测定亚硝酸盐

通讯作者: 牛湘衡,E-mail:niuxiangheng@ujs.edu.cn

English

A Ratiometric Electroanalytical Method Based on Diazotization Reaction for Detection of Nitrite

Corresponding author: NIU Xiang-Heng, niuxiangheng@ujs.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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