双亲聚乙烯醇气凝胶过氧化物酶催化性能及对葡萄糖的传感研究

引用本文: 马崇博, 周明. 双亲聚乙烯醇气凝胶过氧化物酶催化性能及对葡萄糖的传感研究[J]. 分析化学, 2022, 50(4): 535-544. doi: 10.19756/j.issn.0253-3820.221067 shu

Citation: MA Chong-bo, ZHOU Ming. Investigation on Catalytic Performance of Poly（vinyl alcohol） Amphiphilic Aerogel as Peroxidase Mimics and Its Application in Sensing Glucose[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 535-544. doi: 10.19756/j.issn.0253-3820.221067 shu

双亲聚乙烯醇气凝胶过氧化物酶催化性能及对葡萄糖的传感研究

马崇博 ,
周明 ^,

东北师范大学化学学院, 多酸与网状材料化学教育部重点实验室, 动力电池国家地方联合工程实验室, 纳米生物传感与纳米生物分析吉林省高校重点实验室, 分析测试中心, 长春 130024

通讯作者: 周明,E-mail:zhoum@nenu.edu.cn

基金项目:
国家“111”计划项目(No.B18012)

吉林省人力资源和社会保障厅、吉林省教育厅、吉林省高校纳米生物传感与纳米生物分析重点实验室和东北师范大学分析测试中心资助

吉林省自然科学基金项目(No.212558JC010484610)

国家自然科学基金项目(No.22004014)

中央高校基本科研业务费专项资金项目(Nos.2412020QD007,JGPY201802,2412020ZD006,2412019QD008)

摘要: 利用聚乙烯醇(PVA)为基本骨架,马来酸(MA)为辅助交联剂,合成了具有优异类过氧化物酶活性的双亲气凝胶。通过调整马来酸的用量,使气凝胶表面暴露出大量的羧基和酯基,可分别作为酶促反应的催化活性位点和底物3,3’,5,5’-四甲基联苯胺(TMB)、多巴胺及过氧化氢(H₂O₂)的结合位点。利用此气凝胶高效催化H₂O₂氧化TMB的显色反应,建立了葡萄糖的传感检测方法,缓冲体系中的线性检测范围为17.4~80.0μmol/L,检出限(3σ)为17.4μmol/L;稀释人血清中线性检测范围为27.4μmol/L~1.0 mmol/L,检出限(3σ)为27.4μmol/L。利用本方法对人血清中葡萄糖含量进行检测,回收率为96.8%~103.0%,相对标准偏差为0.8%~3.9%,检测结果与商品化血糖仪的检测结果基本一致。本研究创新性地以三维有机气凝胶作为一种新型人工模拟酶,拓宽了人工模拟酶材料的设计思路与合成方式,具有轻量化、机械性能优良等特点,有望用于便携式传感器的构建。

关键词:

English

Investigation on Catalytic Performance of Poly（vinyl alcohol） Amphiphilic Aerogel as Peroxidase Mimics and Its Application in Sensing Glucose

MA Chong-bo ,
ZHOU Ming ^,

Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education, National & Local United Engineering Laboratory for Power Batteries, Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Analysis and Testing Center, Department of Chemistry, Northeast Normal University, Changchun 130024, China

Corresponding author: ZHOU Ming, zhoum@nenu.edu.cn

Received Date: 10 February 2022
Revised Date: 14 March 2022
Fund Project:
the “111” Project of China(No. B18012)

the Jilin Provincial Department of Human Resources and Social Security,the Jilin Provincial Department of Education(China),the Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province(China),Analysis and Testing Center of Northeast Normal University(China)

the Natural Science Foundation of Jilin Province,China(No. 212558JC010484610)

Supported by the National Natural Science Foundation of China(No. 22004014)

the Fundamental Research Funds for the Central Universities of China (Nos. 2412020QD007, JGPY201802, 2412020ZD006, 2412019QD008)

Abstract: A method for synthesis of an amphiphilic aerogel with remarkable peroxidase-like activity was developed by using poly(vinyl alcohol) (PVA) as skeleton and maleic acid(MA) as auxiliary crosslinker.Large amount of carboxyl and ester groups could be exposed on aerogel surface by tuning the dosage of MA, which acted as the active sites and binding sites for the substrate material. A colorimetric method for glucose sensing by the catalyzed chromogenic reaction between 3, 3', 5, 5'-tetramethylbenzidine and H₂O₂, was thus established. The linear detection range of glucose in buffer system was 17.4-80.0 μmol/L, with limit of detection(3σ) of 17.4 μmol/L. While in diluted human serum, the linear detection range was 27.4 μmol/L-1.0 mmol/L, with limit of detection(3σ) of 27.4 μmol/L. This method was used in detection of glucose in human serum, with recoveries of 96.8%-103.0% and relative standard deviations of 0.8%-3.9%, and the detection results were highly consistent with those obtained from the commercial glucose meter. This study innovatively developed a three-dimensional macroscope organic aerogel as a new type of artificial enzyme, broadening the design philosophy and enriching the synthesis strategy. It was promising in construction of portable sensors by virtue of the lightness in weight and eligible mechanical properties.

Key words:

1. [1]
  UNNIKRISHNAN B, LIEN C W, CHU H W, HUANG C C. J. Hazard. Mater., 2021, 401:123397.UNNIKRISHNAN B, LIEN C W, CHU H W, HUANG C C. J. Hazard. Mater., 2021, 401:123397.
2. [2]
  WEI H, WANG E. Anal. Chem., 2008, 80(6):2250-2254.WEI H, WANG E. Anal. Chem., 2008, 80(6):2250-2254.
3. [3]
  LING P, ZHANG Q, CAO T, GAO F. Angew. Chem., Int. Ed., 2018, 57(23):6819-6824.LING P, ZHANG Q, CAO T, GAO F. Angew. Chem., Int. Ed., 2018, 57(23):6819-6824.
4. [4]
  WEI H, GAO L, FAN K, LIU J, HE J, QU X, DONG S, WANG E, YAN X. Nano Today, 2021, 40:101269.WEI H, GAO L, FAN K, LIU J, HE J, QU X, DONG S, WANG E, YAN X. Nano Today, 2021, 40:101269.
5. [5]
  ZHANG R, YAN X, FAN K. Acc. Mater. Res., 2021, 2(7):534-547.ZHANG R, YAN X, FAN K. Acc. Mater. Res., 2021, 2(7):534-547.
6. [6]
  WU J, WANG X, WANG Q, LOU Z, LI S, ZHU Y, QIN L, WEI H. Chem. Soc. Rev., 2019, 48(4):1004-1076.WU J, WANG X, WANG Q, LOU Z, LI S, ZHU Y, QIN L, WEI H. Chem. Soc. Rev., 2019, 48(4):1004-1076.
7. [7]
  GAO L, ZHUANG J, NIE L, ZHANG J, ZHANG Y, GU N, WANG T, FENG J, YANG D, PERRETT S, YAN X.Nat. Nanotechnol., 2007, 2(9):577-583.GAO L, ZHUANG J, NIE L, ZHANG J, ZHANG Y, GU N, WANG T, FENG J, YANG D, PERRETT S, YAN X.Nat. Nanotechnol., 2007, 2(9):577-583.
8. [8]
  CHEN Z, JI H, LIU C, BING W, WANG Z, QU X. Angew. Chem., Int. Ed., 2016, 55(36):10732-10736.CHEN Z, JI H, LIU C, BING W, WANG Z, QU X. Angew. Chem., Int. Ed., 2016, 55(36):10732-10736.
9. [9]
  TAO Y, JU E, REN J, QU X. Adv. Mater., 2015, 27(6):1097-1104.TAO Y, JU E, REN J, QU X. Adv. Mater., 2015, 27(6):1097-1104.
10. [10]
  DAS B, FRANCO J L, LOGAN N, BALASUBRAMANIAN P, KIM M I, CAO C. Nano-Micro Lett,, 2021, 13(1):193.DAS B, FRANCO J L, LOGAN N, BALASUBRAMANIAN P, KIM M I, CAO C. Nano-Micro Lett,, 2021, 13(1):193.
11. [11]
  LIU M, MOU J, XU X, ZHANG F, XIA J, WANG Z. Talanta, 2020, 220:121374.LIU M, MOU J, XU X, ZHANG F, XIA J, WANG Z. Talanta, 2020, 220:121374.
12. [12]
  PRATSINIS A, KELESIDIS G A, ZUERCHER S, KRUMEICH F, BOLISETTY S, MEZZENGA R, LEROUX J C,SOTIRIOU G A. ACS Nano, 2017, 11(12):12210-12218.PRATSINIS A, KELESIDIS G A, ZUERCHER S, KRUMEICH F, BOLISETTY S, MEZZENGA R, LEROUX J C,SOTIRIOU G A. ACS Nano, 2017, 11(12):12210-12218.
13. [13]
  CHENG L, WU F, BAO H, LI F, XU G, ZHANG Y, NIU W. Small, 2021, 17(47):2104083.CHENG L, WU F, BAO H, LI F, XU G, ZHANG Y, NIU W. Small, 2021, 17(47):2104083.
14. [14]
  MANSUR A A P, LEONEL A G, KRAMBROCK K, MANSUR H S. Catal. Today, 2021, DOI: 10.1016/j.cattod.2021.11.018.MANSUR A A P, LEONEL A G, KRAMBROCK K, MANSUR H S. Catal. Today, 2021, DOI: 10.1016/j.cattod.2021.11.018.
15. [15]
  OROZCO J, GARCÍA-GRADILLA V, D’AGOSTINO M, GAO W, CORTÉS A, WANG J. ACS Nano, 2013, 7(1):818-824.OROZCO J, GARCÍA-GRADILLA V, D’AGOSTINO M, GAO W, CORTÉS A, WANG J. ACS Nano, 2013, 7(1):818-824.
16. [16]
  HE F, MI L, SHEN Y, MORI T, LIU S, ZHANG Y. ACS Appl. Mater. Interfaces, 2018, 10(41):35327-35333.HE F, MI L, SHEN Y, MORI T, LIU S, ZHANG Y. ACS Appl. Mater. Interfaces, 2018, 10(41):35327-35333.
17. [17]
  LI S, SHANG L, XU B, WANG S, GU K, WU Q, SUN Y, ZHANG Q, YANG H, ZHANG F, GU L, ZHANG T,LIU H. Angew. Chem., Int. Ed., 2019, 58(36):12624-12631.LI S, SHANG L, XU B, WANG S, GU K, WU Q, SUN Y, ZHANG Q, YANG H, ZHANG F, GU L, ZHANG T,LIU H. Angew. Chem., Int. Ed., 2019, 58(36):12624-12631.
18. [18]
  SHEN J, REES T W, ZHOU Z, YANG S, JI L, CHAO H. Biomaterials, 2020, 251:120079.SHEN J, REES T W, ZHOU Z, YANG S, JI L, CHAO H. Biomaterials, 2020, 251:120079.
19. [19]
  JIN T, LI Y L, JING W J, LI Y C, FAN L Z, LI X H. Chem. Commun., 2020, 56(4):659-662.JIN T, LI Y L, JING W J, LI Y C, FAN L Z, LI X H. Chem. Commun., 2020, 56(4):659-662.
20. [20]
  WEN S H, ZHONG X L, WU Y D, LIANG R P, ZHANG L, QIU J D. Anal. Chem., 2019, 91(10):6487-6497.WEN S H, ZHONG X L, WU Y D, LIANG R P, ZHANG L, QIU J D. Anal. Chem., 2019, 91(10):6487-6497.
21. [21]
  PRASAD S N, WEERATHUNGE P, KARIM M N, ANDERSON S, HASHMI S, MARIATHOMAS P D, BANSAL V, RAMANATHAN R. Anal. Bioanal. Chem., 2021, 413(5):1279-1291.PRASAD S N, WEERATHUNGE P, KARIM M N, ANDERSON S, HASHMI S, MARIATHOMAS P D, BANSAL V, RAMANATHAN R. Anal. Bioanal. Chem., 2021, 413(5):1279-1291.
22. [22]
  XI J Q, WEI G, AN L F, XU Z B, XU Z L, FAN L, GAO L Z. Nano Lett., 2020, 20(1):800-800.XI J Q, WEI G, AN L F, XU Z B, XU Z L, FAN L, GAO L Z. Nano Lett., 2020, 20(1):800-800.
23. [23]
  SINGH N, NAVEENKUMAR S K, GEETHIKA M, MUGESH G. Angew. Chem., Int. Ed., 2021, 60(6):3121-3130.SINGH N, NAVEENKUMAR S K, GEETHIKA M, MUGESH G. Angew. Chem., Int. Ed., 2021, 60(6):3121-3130.
24. [24]
  YILDIRIM D, GOKCAL B, BUBER E, KIP C, DEMIR M C, TUNCEL A. Chem. Eng. J., 2021, 403:126357.YILDIRIM D, GOKCAL B, BUBER E, KIP C, DEMIR M C, TUNCEL A. Chem. Eng. J., 2021, 403:126357.
25. [25]
  MA C B, XU Y, WU L, WANG Q, ZHENG J J, REN G, WANG X, GAO X, ZHOU M, WANG M, WEI H. Angew.Chem,. Int. Ed., 2022, DOI: 10.1002/ange.202116170.MA C B, XU Y, WU L, WANG Q, ZHENG J J, REN G, WANG X, GAO X, ZHOU M, WANG M, WEI H. Angew.Chem,. Int. Ed., 2022, DOI: 10.1002/ange.202116170.
26. [26]
  WANG L, GAO F, WANG A, CHEN X, LI H, ZHANG X, ZHENG H, JI R, LI B, YU X, LIU J, GU Z, CHEN F,CHEN C. Adv. Mater., 2020, 32(48):2005423.WANG L, GAO F, WANG A, CHEN X, LI H, ZHANG X, ZHENG H, JI R, LI B, YU X, LIU J, GU Z, CHEN F,CHEN C. Adv. Mater., 2020, 32(48):2005423.
27. [27]
  WANG X, SUN X Y, BU T, WANG Q Z, ZHANG H, JIA P, LI L W, WANG L. Acta Biomater., 2021, 135:342-355.WANG X, SUN X Y, BU T, WANG Q Z, ZHANG H, JIA P, LI L W, WANG L. Acta Biomater., 2021, 135:342-355.
28. [28]
  WANG Y G, WANG Y Y, WANG F Z, CHI H, ZHAO G H, ZHANG Y, LI T D, WEI Q. J. Colloid Interface Sci.,2022, 606:510-517.WANG Y G, WANG Y Y, WANG F Z, CHI H, ZHAO G H, ZHANG Y, LI T D, WEI Q. J. Colloid Interface Sci.,2022, 606:510-517.
29. [29]
  VERNEKAR A A, SINHA D, SRIVASTAVA S, PARAMASIVAM P U, D’SILVA P, MUGESH G. Nat. Commun.,2014, 5:5301.VERNEKAR A A, SINHA D, SRIVASTAVA S, PARAMASIVAM P U, D’SILVA P, MUGESH G. Nat. Commun.,2014, 5:5301.
30. [30]
  CHEN J X, WU W W, HUANG L, MA Q, DONG S J. Chem.-Eur. J., 2019, 25(51):11940-11944.CHEN J X, WU W W, HUANG L, MA Q, DONG S J. Chem.-Eur. J., 2019, 25(51):11940-11944.
31. [31]
  DAVID M, SERBAN A, RADULESCU C, DANET A F, FLORESCU M. Bioelectrochemistry, 2019, 129:124-134.DAVID M, SERBAN A, RADULESCU C, DANET A F, FLORESCU M. Bioelectrochemistry, 2019, 129:124-134.
32. [32]
  JIANG Z X, LI H, DENG Y Q, HE Y. ACS Sustainable Chem. Eng., 2020, 8(13):5076-5081.JIANG Z X, LI H, DENG Y Q, HE Y. ACS Sustainable Chem. Eng., 2020, 8(13):5076-5081.
33. [33]
  KARIM M N, ANDERSON S R, SINGH S, RAMANATHAN R, BANSAL V. Biosens. Bioelectron., 2018, 110:8-15.KARIM M N, ANDERSON S R, SINGH S, RAMANATHAN R, BANSAL V. Biosens. Bioelectron., 2018, 110:8-15.
34. [34]
  JIA Z, YUAN X Y, WEI J A, GUO X, GONG Y C, LI J, ZHOU H, ZHANG L, LIU J. ACS Appl. Mater. Interfaces,2021, 13(42):49602-49613.JIA Z, YUAN X Y, WEI J A, GUO X, GONG Y C, LI J, ZHOU H, ZHANG L, LIU J. ACS Appl. Mater. Interfaces,2021, 13(42):49602-49613.
35. [35]
  ZENG R J, LUO Z B, ZHANG L J, TANG D P. Anal. Chem., 2018, 90(20):12299-12306.ZENG R J, LUO Z B, ZHANG L J, TANG D P. Anal. Chem., 2018, 90(20):12299-12306.
36. [36]
  ZHANG Y, LIU Q Y, MA C B, WANG Q Q, YANG M T, DU Y. Theranostics, 2020, 10(11):5064-5073.ZHANG Y, LIU Q Y, MA C B, WANG Q Q, YANG M T, DU Y. Theranostics, 2020, 10(11):5064-5073.
37. [37]
  DING H, HU B, ZHANG B, ZHANG H, YAN X Y, NIE G H, LIANG M M. Nano Res., 2021, 14(3):570-583.DING H, HU B, ZHANG B, ZHANG H, YAN X Y, NIE G H, LIANG M M. Nano Res., 2021, 14(3):570-583.
38. [38]
  FAN K L, XI J Q, FAN L, WANG P X, ZHU C H, TANG Y, XU X D, LIANG M M, JIANG B, YAN X Y, GAO L Z.Nat. Commun., 2018, 9:1440.FAN K L, XI J Q, FAN L, WANG P X, ZHU C H, TANG Y, XU X D, LIANG M M, JIANG B, YAN X Y, GAO L Z.Nat. Commun., 2018, 9:1440.
39. [39]
  SINGH N, SAVANUR M A, SRIVASTAVA S, D’SILVA P, MUGESH G. Angew. Chem., Int. Ed., 2017, 56(45):14267-14271.SINGH N, SAVANUR M A, SRIVASTAVA S, D’SILVA P, MUGESH G. Angew. Chem., Int. Ed., 2017, 56(45):14267-14271.
40. [40]
  SUN H, ZHAO A, GAO N, LI K, REN J, QU X. Angew. Chem., Int. Ed., 2015, 54(24):7176-7180.SUN H, ZHAO A, GAO N, LI K, REN J, QU X. Angew. Chem., Int. Ed., 2015, 54(24):7176-7180.
41. [41]
  KISTLER S S. Nature, 1931, 127:741.KISTLER S S. Nature, 1931, 127:741.
42. [42]
  GAO X D, HUANG Y D, ZHANG T T, WU Y Q, LI X M. J. Mater. Chem. A, 2017, 5(25):12856-12862.GAO X D, HUANG Y D, ZHANG T T, WU Y Q, LI X M. J. Mater. Chem. A, 2017, 5(25):12856-12862.
43. [43]
  JIANG F, HSIEH Y L. J. Mater. Chem. A, 2014, 2(18):6337-6342.JIANG F, HSIEH Y L. J. Mater. Chem. A, 2014, 2(18):6337-6342.
44. [44]
  SONG X, CHEN Y, RONG M, XIE Z, ZHAO T, WANG Y, CHEN X, WOLFBEIS O S. Angew. Chem., Int. Ed.,2016, 55(12):3936-3941.SONG X, CHEN Y, RONG M, XIE Z, ZHAO T, WANG Y, CHEN X, WOLFBEIS O S. Angew. Chem., Int. Ed.,2016, 55(12):3936-3941.
45. [45]
  YUAN Y, ZHANG C, WANG C, CHEN M. J. Solid State Electrochem., 2015, 19(2):619-627.YUAN Y, ZHANG C, WANG C, CHEN M. J. Solid State Electrochem., 2015, 19(2):619-627.
46. [46]
  MA C B, ZHANG Y, LIU Q, DU Y, WANG E. Anal. Chem., 2020, 92(7):5319-5328.MA C B, ZHANG Y, LIU Q, DU Y, WANG E. Anal. Chem., 2020, 92(7):5319-5328.
47. [47]
  MA C B, DU B, WANG E. Adv. Funct. Mater., 2017, 27(10):1604423.MA C B, DU B, WANG E. Adv. Funct. Mater., 2017, 27(10):1604423.
48. [48]
  LING P, ZHANG Q, CAO T, GAO F. Angew. Chem., Int. Ed., 2018, 57(23):6819-6824.LING P, ZHANG Q, CAO T, GAO F. Angew. Chem., Int. Ed., 2018, 57(23):6819-6824.
49. [49]
  ZHANG Y, WU C, ZHOU X, WU X, YANG Y, WU H, GUO S, ZHANG J. Nanoscale, 2013, 5(5):1816-1819.ZHANG Y, WU C, ZHOU X, WU X, YANG Y, WU H, GUO S, ZHANG J. Nanoscale, 2013, 5(5):1816-1819.
50. [50]
  FAN K, XI J, FAN L, WANG P, ZHU C, TANG Y, XU X, LIANG M, JIANG B, YAN X, GAO L. Nat. Commun.,2018, 9:1440.FAN K, XI J, FAN L, WANG P, ZHU C, TANG Y, XU X, LIANG M, JIANG B, YAN X, GAO L. Nat. Commun.,2018, 9:1440.
51. [51]
  ZHANG P, SUN D, CHO A, WEON S, LEE S, LEE J, HAN J W, KIM D P, CHOI W. Nat. Commun., 2019, 10:940.ZHANG P, SUN D, CHO A, WEON S, LEE S, LEE J, HAN J W, KIM D P, CHOI W. Nat. Commun., 2019, 10:940.
52. [52]
  VALLABANI N V S, KARAKOTI A S, SINGH S. Colloids Surf., B, 2017, 153:52-60.VALLABANI N V S, KARAKOTI A S, SINGH S. Colloids Surf., B, 2017, 153:52-60.
53. [53]
  TAO Y, LIN Y, HUANG Z, REN J, QU X. Adv. Mater., 2013, 25(18):2594-2599.TAO Y, LIN Y, HUANG Z, REN J, QU X. Adv. Mater., 2013, 25(18):2594-2599.
54. [54]
  GUO Y, DENG L, LI J, GUO S, WANG E, DONG S. ACS Nano, 2011, 5(2):1282-1290.GUO Y, DENG L, LI J, GUO S, WANG E, DONG S. ACS Nano, 2011, 5(2):1282-1290.

扫一扫看文章

计量

PDF下载量: 8
文章访问数: 662
HTML全文浏览量: 61

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

双亲聚乙烯醇气凝胶过氧化物酶催化性能及对葡萄糖的传感研究

通讯作者: 周明,E-mail:zhoum@nenu.edu.cn

English

Investigation on Catalytic Performance of Poly（vinyl alcohol） Amphiphilic Aerogel as Peroxidase Mimics and Its Application in Sensing Glucose

Corresponding author: ZHOU Ming, zhoum@nenu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

双亲聚乙烯醇气凝胶过氧化物酶催化性能及对葡萄糖的传感研究

通讯作者: 周明,E-mail:zhoum@nenu.edu.cn

English

Investigation on Catalytic Performance of Poly（vinyl alcohol） Amphiphilic Aerogel as Peroxidase Mimics and Its Application in Sensing Glucose

Corresponding author: ZHOU Ming, zhoum@nenu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs