Citation: HAN Xiao-Hong, HAN Xi, WANG Rong-Rong, SHI Chun-Zhen. Metabolic Profiling Analysis of Formaldehyde Degrading Strain of XF-1 Using Gas Chromatography-Time of Flight-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 1072-1082. doi: 10.19756/j.issn.0253-3820.221050 shu

Metabolic Profiling Analysis of Formaldehyde Degrading Strain of XF-1 Using Gas Chromatography-Time of Flight-Mass Spectrometry

HAN Xiao-Hong ¹ ,
HAN Xi ¹ ,
WANG Rong-Rong ¹ ,
SHI Chun-Zhen ^1,2,3,4,,

1.
School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China;
2.
State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing 100048, China;
3.
Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China;
4.
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

Corresponding author: SHI Chun-Zhen, shichunzhen@btbu.edu.cn
Received Date: 25 January 2022
Revised Date: 21 April 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.52170106) and the High-level Teacher in Beijing Municipal Universities in the Period of 13th Five-year Plan (No.CIT&TCD201904032).

Cooking oil fumes are widely concerned because of the complex composition and the hard-to-degrade characterization. One of the most important pollutants in cooking oil fumes is formaldehyde which is a hazard to human health. In this study, a strain of Bacillus amyloliticus XF-1 screened out in previous work was used for efficiently degrading formaldehyde from the condensate of cooking oil fumes. The previous study found that when formaldehyde was the only carbon source, the biodegradation rate was reduced owing to the inhibition effect to the strain. It was necessary to investigate the metabolic mechanism of strain XF-1 and to illustrate the reason for growth inhibition. The metabolic profiling of XF-1 with different carbon sources and under different culture time conditions was analyzed by gas chromatography-time of flight-mass spectrometry (GC-TOF-MS). The results showed that different carbon sources caused different metabolic profiles of XF-1. The metabolic process was delayed using formaldehyde as the carbon source. The concentrations of cystine, aspartic acid and glutamine were significantly changed, which showed that the cell growth and proliferation were inhibited. This work provided a reference for improving the biodegradation efficiency of cooking oil fumes in the future.
- Bacillus amyloliquefaciens,
- Formaldehyde degrading strain,
- Metabolomics,
- Gas chromatography-time of flight-mass spectrometry
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