Citation: MA Ru-long, HAO Qian, LIANG Meng-jia, YU Yan-kai, NIU Na, CHEN Li-gang. Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 545-553. doi: 10.19756/j.issn.0253-3820.221035 shu

Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China

Corresponding author: NIU Na, CHEN Li-gang,
Received Date: 19 January 2022
Revised Date: 28 February 2022

Fund Project: the Natural Science Foundation of Heilongjiang Province,China(No. B2008001)Supported by the National Natural Science Foundation of China(No. 21401019)the Provincial College Students’ Innovation Training Program of Northeast Forestry University(No. 202110225341)

The MoS₂-modified straw carbon dots nanozyme(Mo, S-CDs) was prepared by hydrothermal synthesis for detection of content of uric acid in human urine. This work fully exploited the inherent advantages of straw structure and realized the recycling of biomass waste. The characterization of Mo, S-CDs showed that Mo and S elements were successfully modified into the structure of straw carbon dots, and the Mo, S-CDs were spherical with abundant functional groups on the surface to ensure their good dispersion in aqueous medium and amorphous carbon structure at crystal level. The Mo, S-CDs had the activity of mimetic peroxidase and showed high catalytic efficiency. They were able to rapidly catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine in a system containing H₂O₂ to generate chromogenic product, which visually showed a characteristic blue color signal in response and reached a peak absorbance at 652 nm.The cascade catalytic sensing system of uric acid-Mo, S-CDs was constructed based on the sensing ability of Mo, S-CDs for H₂O₂ generation catalyzed by uric acid, which was applied to the detection of uric acid in human urine. The sensing system had good linear range(5-100 μmol/L), low detection limit(1.8 μmol/L), satisfied recovery(96.8%-106.1%) and good relative standard deviation(less than 5%) in detection of uric acid. The results showed that Mo, S-CDs had advantages over natural peroxidases in terms of wide applicable temperature range and high stability, which would make them more widely useful in fields such as life analysis.
- Carbon dots nanozyme,
- Straw,
- Peroxidase,
- Uric acid,
- Urine
1. [1]
  AGNOLETTI D, CICERO A F G, BORGHI C. Cardiol. Clin., 2021, 39(3):365-376.
2. [2]
  HAN M, KIM H, KIM H J, KANG E, KIM Y S, CHOIK H, KIM S W, AHN C, OH K H. BMC Nephrol., 2021,22(1):247.
3. [3]
4. [4]
  JEN J F, HSIAO S L, LIU K H. Talanta, 2002, 58(4):711-717.
5. [5]
6. [6]
7. [7]
8. [8]
  ZHAO J J, ZHAO L M, LAN C Q, ZHAO S L. Sens. Actuators, B, 2016, 223:246-251.
9. [9]
  WU C Y, ZHU L J, LU Q J, LI H T, ZHANG Y Y, YAO S Z. Microchim. Acta, 2019, 186(12):754-761.
10. [10]
  BADOEI-DALFARD A, SOHRABI N, KARAMI Z, SARGAZI G. Biosens. Bioelectron., 2019, 141:111420.
11. [11]
12. [12]
  SUN Y, CHEN J, QI H, SHI Y. J. Chromatogr. B, 2015, 1004:53-59.
13. [13]
  LIU H, GU C, HOU C, YIN Z, FAN K, ZHANG M. Sens. Actuators, B, 2016, 224:857-862.
14. [14]
  FANG A, WU Q, LU Q, CHEN H, LI H, LIU M, ZHANG Y, YAO S. Biosens. Bioelectron., 2016, 86:664-670.
15. [15]
  PAN Y D, YANG Y F, PANG Y J, SHI Y, LONG Y J, ZHENG H Z. Talanta, 2018, 185:433-438.
16. [16]
  SUN H J, ZHOU Y, REN J S, QU X G. Angew. Chem., Int. Ed., 2018, 57(30):9224-9237.
17. [17]
  GAO L Z, ZHUANG J, NIE L, ZHANG J B, ZHANG Y, GU N, WANG T H, FENG J, YANG D L, PERRETT S,YAN X Y. Nat. Nanotechnol., 2007, 2(9):577-583.
18. [18]
  DING H, HU B, ZHANG B, ZHANG H, YAN X Y, NIE G H, LIANG M M. Nano Res., 2021, 14:570-583.
19. [19]
20. [20]
21. [21]
  DEVI P, SAINI S, KIM K H. Biosens. Bioelectron., 2019, 141:111158.
22. [22]
  TAN J Y, LI Y, TAN X, WU H G, LI H, YANG S. Front. Chem., 2021, 9:696030.
23. [23]
  LI F, HE T S, WU S H, PENG Z J, QIU P, TANG X M. Microchem. J., 2021, 164:105987.
24. [24]
  DING H, WEI J S, ZHANG P, ZHOU Z Y, GAO Q Y, XIONG H M. Small, 2018, 14(22):1800612.
25. [25]
  WANG X J, WU Q, JIANG K L, WANG C X, ZHANG C. Sens. Actuators, B, 2017, 252:183-190.
26. [26]
  BANO D, KUMAR V, SINGH V K, HASAN S H. New J. Chem., 2018, 42(8):5814-5821.
27. [27]
  ZHAO Y H, HUANG Y, WU J L, ZHAN X I, XIE Y Y, TANG D Y, CAO H Y, YUN Y. RSC Adv., 2018, 8(13):7252-7259.
28. [28]
  JIANG B, DUAN D M, GAO L Z, ZHOU M J, FAN K L, TANG Y, XI J Q, BI Y H, TONG Z, GAO G F, XIE N,TANG A F, NIE G H, LIANG M M, YAN X Y. Nat. Protoc., 2018, 13(7):1506-1520.
29. [29]
  HUANG Z, HE W T, SHEN H, HAN G J, WANG H, SU P, SONG J Y, YANG Y. Talanta, 2021, 230:122337.
30. [30]
  CHEN Q, LIU M L, ZHAO J N, PENG X, CHEN X J, MI N X, YIN B D, LI H T, ZHANG Y Y, YAO S Z. Chem.Commun., 2014, 50:6771-6774.
31. [31]
  LIN L P, SONG X H, CHEN Y Y, RONG M C, ZHAO T T, WANG Y R, JIANG Y Q, CHEN X. Anal. Chim. Acta,2015, 869:89-95.
32. [32]
  DING C P, YAN Y H, XIANG D S, ZHANG C L, XIAN Y Z. Microchim. Acta, 2016, 183:625-631.
33. [33]
  JIN L H, MENG Z, ZHANG Y Q, CAI S J, ZHANG Z H, LI C, SHANG L, SHEN Y H. ACS Appl. Mater.Interfaces, 2017, 9(11):10027–10033.
34. [34]
  NIRALA N R, VINITA, PRAKASH R. Microchim. Acta, 2018, 185(4):224.
35. [35]
  SUN H J, ZHAO A D, GAO N, LI K, REN J S, QU X G. Angew. Chem., Int. Ed., 2015, 54(24):7176-7180.
1. [1]
  Hao BAI , Weizhi JI , Jinyan CHEN , Hongji LI , Mingji LI . Preparation of Cu₂O/Cu-vertical graphene microelectrode and detection of uric acid/electroencephalogram. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1309-1319. doi: 10.11862/CJIC.20240001
2. [2]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
3. [3]
  Rong Tian , Yadi Yang , Naihao Lu . Comprehensive Experimental Design of Undergraduate Students Based on Interdisciplinarity: Study on the Effect of Quercetin on Chlorination Activity of Myeloperoxidase. University Chemistry, 2024, 39(8): 247-254. doi: 10.3866/PKU.DXHX202312064
4. [4]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
5. [5]
  Hongyao Li , Youyan Liu , Luwei Dai , Min Yang , Qihui Wang . The Blessing of Indium Sulfide：Confronting the Narrow Path with Uric Acid. University Chemistry, 2024, 39(5): 325-335. doi: 10.3866/PKU.DXHX202311104
6. [6]
  Xiaofeng Zhu , Bingbing Xiao , Jiaxin Su , Shuai Wang , Qingran Zhang , Jun Wang . Transition Metal Oxides/Chalcogenides for Electrochemical Oxygen Reduction into Hydrogen Peroxides. Acta Physico-Chimica Sinica, 2024, 40(12): 2407005-. doi: 10.3866/PKU.WHXB202407005
7. [7]
  Wenli FENG , Lu ZHAO , Yunfeng BAI , Feng FENG . Research progress on ultralong room temperature phosphorescent carbon dots. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 833-846. doi: 10.11862/CJIC.20240308
8. [8]
  Ke Li , Chuang Liu , Jingping Li , Guohong Wang , Kai Wang . 钛酸铋/氮化碳无机有机复合S型异质结纯水光催化产过氧化氢. Acta Physico-Chimica Sinica, 2024, 40(11): 2403009-. doi: 10.3866/PKU.WHXB202403009
9. [9]
  Jiaxi Xu , Yuan Ma . Influence of Hyperconjugation on the Stability and Stable Conformation of Ethane, Hydrazine, and Hydrogen Peroxide. University Chemistry, 2024, 39(11): 374-377. doi: 10.3866/PKU.DXHX202402049
10. [10]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
11. [11]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
12. [12]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
13. [13]
  Junli Liu . Practice and Exploration of Research-Oriented Classroom Teaching in the Integration of Science and Education: a Case Study on the Synthesis of Sub-Nanometer Metal Oxide Materials and Their Application in Battery Energy Storage. University Chemistry, 2024, 39(10): 249-254. doi: 10.12461/PKU.DXHX202404023
14. [14]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
15. [15]
  Zhuo WANG , Xiaotong LI , Zhipeng HU , Junqiao PAN . Three-dimensional porous carbon decorated with nano bismuth particles: Preparation and sodium storage properties. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 267-274. doi: 10.11862/CJIC.20240223
16. [16]
  Kai CHEN , Fengshun WU , Shun XIAO , Jinbao ZHANG , Lihua ZHU . PtRu/nitrogen-doped carbon for electrocatalytic methanol oxidation and hydrogen evolution by water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1357-1367. doi: 10.11862/CJIC.20230350
17. [17]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
18. [18]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
19. [19]
  Jinyi Sun , Lin Ma , Yanjie Xi , Jing Wang . Preparation and Electrocatalytic Nitrogen Reduction Performance Study of Vanadium Nitride@Nitrogen-Doped Carbon Composite Nanomaterials: A Recommended Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(4): 184-191. doi: 10.3866/PKU.DXHX202310094
20. [20]
  Yueguang Chen , Wenqiang Sun . “Carbon” Adventures. University Chemistry, 2024, 39(9): 248-253. doi: 10.3866/PKU.DXHX202308074

Get Citation

PDF

XML

Metrics

PDF Downloads(12)
Abstract views(618)
HTML views(78)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of MoS2-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

Metrics

通讯作者: 陈斌, bchen63@163.com

Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine