Citation: HAN Qing-Yi, SHAO Ming-Zheng, ZHANG Ding-Ding, LI Yu-Yan, DU Pei-Yao, ZHANG Li-Bing, LU Xiao-Quan. DNA-functionalized Metal-organic Frameworks-based Fluorescent Probe for Detection and Imaging of Flap Endonuclease 1[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(1): 53-62. doi: 10.19756/j.issn.0253-3820.221034 shu

DNA-functionalized Metal-organic Frameworks-based Fluorescent Probe for Detection and Imaging of Flap Endonuclease 1

1.
Tianjin Key Laboratory of Molecular Optoelectronic Science, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China;
2.
Key Laboratory of Bioelectrochemistry&Environmental Analysis of Gansu Province, College of Chemistry&Chemical Engineering, Northwest Normal University, Lanzhou 730070, China

Corresponding author: ZHANG Li-Bing, LU Xiao-Quan,
Received Date: 19 January 2022
Revised Date: 17 October 2022

Fund Project: Supported by the National Natural Science Foundation of China (Nos.22174110,22127803,21904095), the Industrial Support Plan of Gansu Provincial Department of Education (No.2021cyzc-01), the Program of Tianjin Science and Technology Major Project and Engineering (No.19ZXYXSY00090) and the Special Fund Project for Guiding Local Scientific and Technological Development by the Central Government of China (No.2020-2060503-17).

Structure-specific nuclease (FEN1) is overexpressed in a variety of cancer cells and is considered as a potential biomarker for cancer diagnosis. Accurate detection of FEN1 is of great significance for the early diagnosis and prognosis of cancer. Fluorescence strategies have been widely used in the detection of FEN1 in recent years due to their simple operation and high sensitivity, but the strategy for detecting FEN1 based on ratiometric fluorescence signals has not been reported. Here, a ratiometric fluorescent probe (FAM-RhB@UiO-66-NH₂) based on DNA-functionalized metal-organic frameworks (MOFs) was designed for the first time for detection and imaging of intracellular FEN1. Among them, Rhodamine B (RhB) was encapsulated into MOFs, and the fluorophore FAM-modified DNA strand was combined with MOFs through Zr-O-P interaction to form a nanoprobe based on fluorescence resonance energy transfer (FRET). In the presence of FEN1, the DNA strand was cleaved by the enzyme to induce the destruction of the FRET system, resulting in the change of the fluorescence ratio to achieve the detection of FEN1. Under the optimal experimental conditions, the linear range for detection of FEN1 was 0.01-3.0 U, the linear equation was y=0.1314x+1.61071, and the detection limit was as low as 0.004 U (3σ). By using FAM-RhB@UiO-66-NH₂ as probe, intracellular imaging of FEN1 could distinguish cancer cells from normal cells.
- Fluorescence sensing,
- Metal-organic framework,
- Flap endonuclease 1,
- Deoxyribonucleic acid,
- Detection,
- Imaging
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