基于DNA功能化金属有机框架材料的荧光探针对瓣状核酸内切酶1的检测和成像研究

韩庆怡 邵明政 张丁丁 李雨彦 杜佩瑶 张立兵 卢小泉

引用本文: 韩庆怡, 邵明政, 张丁丁, 李雨彦, 杜佩瑶, 张立兵, 卢小泉. 基于DNA功能化金属有机框架材料的荧光探针对瓣状核酸内切酶1的检测和成像研究[J]. 分析化学, 2023, 51(1): 53-62. doi: 10.19756/j.issn.0253-3820.221034 shu
Citation:  HAN Qing-Yi,  SHAO Ming-Zheng,  ZHANG Ding-Ding,  LI Yu-Yan,  DU Pei-Yao,  ZHANG Li-Bing,  LU Xiao-Quan. DNA-functionalized Metal-organic Frameworks-based Fluorescent Probe for Detection and Imaging of Flap Endonuclease 1[J]. Chinese Journal of Analytical Chemistry, 2023, 51(1): 53-62. doi: 10.19756/j.issn.0253-3820.221034 shu

基于DNA功能化金属有机框架材料的荧光探针对瓣状核酸内切酶1的检测和成像研究

    通讯作者: 张立兵,E-mail:libing.zhang@tju.edu.cn; 卢小泉,E-mail:luxq@nwnu.edu.cn
  • 基金项目:

    国家自然科学基金项目(Nos.22174110,22127803,21904095)、甘肃省高等学校产业支撑计划项目(No.2021cyzc-01)、天津市科技重大专项与工程项目(No.19ZXYXSY00090)和中央政府引导地方科技发展专项资金项目(No.2020-2060503-17)资助。

摘要: 结构特异性核酸酶瓣状核酸内切酶1(Flap enduclease 1,FEN1)在多种癌细胞中过度表达,被认为是癌症临床诊断的潜在生物标志物。FEN1的精确检测对于癌症早期诊断和预后具有重要意义。荧光法具有操作简单、灵敏度高等优势,被广泛应用于FEN1检测,但基于比率荧光信号检测FEN1的策略尚未见报道。本研究设计了一种基于DNA功能化金属有机框架材料(MOFs)的比率荧光探针(FAM-RhB@UiO-66-NH2),用于细胞内FEN1的检测和成像。其中,罗丹明B(RhB)被封装在MOFs中,荧光团FAM修饰的DNA链通过Zr—O—P相互作用与MOFs结合,形成基于荧光共振能量转移(FRET)的纳米探针。在FEN1存在的情况下,DNA链被酶切割,FRET体系被破坏,荧光比率发生变化,从而可实现对FEN1的检测。在最佳实验条件下,检测FEN1的线性范围为0.01~3.0U,线性方程为y=0.1314x+1.6107,检出限低至0.004U(3σ)。将此探针用于细胞内FEN1成像,能够区分癌细胞和正常细胞。

English


    1. [1]

      TANG Y F, WEI W, LIU Y, LIU S Q. Anal. Chem., 2021, 93(11):4960-4966.TANG Y F, WEI W, LIU Y, LIU S Q. Anal. Chem., 2021, 93(11):4960-4966.

    2. [2]

      KIN C Y, PARK M S, DYERR B. Biochemistry, 2001, 40(10):3208-3214.KIN C Y, PARK M S, DYERR B. Biochemistry, 2001, 40(10):3208-3214.

    3. [3]

      YANG H T, WANG C C, XU E S, WEI W, LIU Y, LIU S Q. Anal. Chem., 2021, 93(16):6567-6572.YANG H T, WANG C C, XU E S, WEI W, LIU Y, LIU S Q. Anal. Chem., 2021, 93(16):6567-6572.

    4. [4]

      ZHENG L, DAI H F, ZHOU M, LI M, SINGH P, QIU J Z, TSARK W, HUANG Q, KERNSTINE K, ZHANG X M, LIN D X, SHEN B H. Nat. Med., 2007, 13:812-819.ZHENG L, DAI H F, ZHOU M, LI M, SINGH P, QIU J Z, TSARK W, HUANG Q, KERNSTINE K, ZHANG X M, LIN D X, SHEN B H. Nat. Med., 2007, 13:812-819.

    5. [5]

      HE L F, LUO L B, ZHU H, YANG H, ZHANG Y L, WU H, SUN H F, JIANG F, KATHERA C S, LIU L J, ZHUANG Z H, CHEN H Y, PAN F Y, HU Z G, ZHANG J, GUO Z G. Mol. Oncol., 2017, 11(6):640-654.HE L F, LUO L B, ZHU H, YANG H, ZHANG Y L, WU H, SUN H F, JIANG F, KATHERA C S, LIU L J, ZHUANG Z H, CHEN H Y, PAN F Y, HU Z G, ZHANG J, GUO Z G. Mol. Oncol., 2017, 11(6):640-654.

    6. [6]

      ZOU J, ZHU L L, JIANG X M, WANG Y, WANG Y, WANG X W, CHEN B. Oncotarget, 2018, 9:11268-11278.ZOU J, ZHU L L, JIANG X M, WANG Y, WANG Y, WANG X W, CHEN B. Oncotarget, 2018, 9:11268-11278.

    7. [7]

      ABDEL-FATAH T A, RUSSELL R, ALBARAKATI N, MALONEY D J, DORJSUREN D, RUEDA O M, MOSELEY P, MOHAN V, SUN H M, ABBOTTS R, MUKHERJEE A, AGARWAL D, ILLUZZI J L, JADHAV A, SIMEONOV A, BALL G, CHAN S, CALDAS C, ELLIS I O, WILSONIII D M, MADHUSUDAN S. Mol. Oncol., 2014, 8(7):1326-1338.ABDEL-FATAH T A, RUSSELL R, ALBARAKATI N, MALONEY D J, DORJSUREN D, RUEDA O M, MOSELEY P, MOHAN V, SUN H M, ABBOTTS R, MUKHERJEE A, AGARWAL D, ILLUZZI J L, JADHAV A, SIMEONOV A, BALL G, CHAN S, CALDAS C, ELLIS I O, WILSONIII D M, MADHUSUDAN S. Mol. Oncol., 2014, 8(7):1326-1338.

    8. [8]

      WANG K J, XIE C H, CHEN D R. Int. J. Mol. Med., 2014, 33(5):1268-1274.WANG K J, XIE C H, CHEN D R. Int. J. Mol. Med., 2014, 33(5):1268-1274.

    9. [9]

      MA L, CAO X Q, WANG H Y, LU K, WANG Y, TU C H, DAI Y J, MENG Y Y, LI Y Y, YU P, MAN S L, DIAO A P. J. Agric. Food Chem., 2019, 67(6):1656-1665.MA L, CAO X Q, WANG H Y, LU K, WANG Y, TU C H, DAI Y J, MENG Y Y, LI Y Y, YU P, MAN S L, DIAO A P. J. Agric. Food Chem., 2019, 67(6):1656-1665.

    10. [10]

      ZHANG H, BA S, MAHAJAN D, LEE J Y, YE R J, SHAO F W, LU L, LI T H. Nano Lett., 2018, 18(11):7383-7388.ZHANG H, BA S, MAHAJAN D, LEE J Y, YE R J, SHAO F W, LU L, LI T H. Nano Lett., 2018, 18(11):7383-7388.

    11. [11]

      WANG C C, ZHANG D D, TANG Y F, WEI W, LIU Y, LIU S Q. ACS Appl. Bio Mater., 2020, 3(7):4573-4580.WANG C C, ZHANG D D, TANG Y F, WEI W, LIU Y, LIU S Q. ACS Appl. Bio Mater., 2020, 3(7):4573-4580.

    12. [12]

      ZHANG G Y, SHAN D, DONG H F, COSNIER S, AL-GHANIM K A, AHMAD Z, MAHBOOB S, ZHANG X J. Anal. Chem., 2018, 90(20):12284-12291.ZHANG G Y, SHAN D, DONG H F, COSNIER S, AL-GHANIM K A, AHMAD Z, MAHBOOB S, ZHANG X J. Anal. Chem., 2018, 90(20):12284-12291.

    13. [13]

      BAO T, FU R B, WEN W, ZHANG X H, WANG S F. ACS Appl. Mater. Interfaces, 2020, 12(2):2087-2094.BAO T, FU R B, WEN W, ZHANG X H, WANG S F. ACS Appl. Mater. Interfaces, 2020, 12(2):2087-2094.

    14. [14]

      WANG Z J, FU Y, KANG Z Z, LIU X G, CHEN N, WANG Q, TU Y Q, WANG L H, SONG S P, LING D S, SONG H Y, KONG X Q, FAN C H. J. Am. Chem. Soc., 2017, 139(44):15784-15791.WANG Z J, FU Y, KANG Z Z, LIU X G, CHEN N, WANG Q, TU Y Q, WANG L H, SONG S P, LING D S, SONG H Y, KONG X Q, FAN C H. J. Am. Chem. Soc., 2017, 139(44):15784-15791.

    15. [15]

      TERZOPOULOU A, NICHOLAS J D, CHEN X Z, NELSON B J, PANE S, PUIGMARTI-LUIS J. Chem. Rev., 2020, 120(20):11175-11193.TERZOPOULOU A, NICHOLAS J D, CHEN X Z, NELSON B J, PANE S, PUIGMARTI-LUIS J. Chem. Rev., 2020, 120(20):11175-11193.

    16. [16]

      SATYAVOLU N S R, LOH K Y, TAN L H, LU Y. Small, 2019, 15(26):1900975.SATYAVOLU N S R, LOH K Y, TAN L H, LU Y. Small, 2019, 15(26):1900975.

    17. [17]

      PAN X B, XU H T, ZHAO X, ZHANG H Q. ACS Sustainable Chem. Eng., 2020, 8(2):1087-1094.PAN X B, XU H T, ZHAO X, ZHANG H Q. ACS Sustainable Chem. Eng., 2020, 8(2):1087-1094.

    18. [18]

      GAO N, HUANG J, WANG L Y, FENG J Y, HUANG P C, WU F Y. Appl. Surf. Sci., 2018, 459(30):686-692.GAO N, HUANG J, WANG L Y, FENG J Y, HUANG P C, WU F Y. Appl. Surf. Sci., 2018, 459(30):686-692.

    19. [19]

      KONG X J, JI X T, HE T, XIE L H, ZHANG Y Z, LV H Y, DING C F, LI J R. ACS Appl. Mater. Interfaces, 2020, 12(31):35375-35384.KONG X J, JI X T, HE T, XIE L H, ZHANG Y Z, LV H Y, DING C F, LI J R. ACS Appl. Mater. Interfaces, 2020, 12(31):35375-35384.

    20. [20]

      LIU B W, LIU J W. J. Am. Chem. Soc., 2017, 139(28):9471-9474.LIU B W, LIU J W. J. Am. Chem. Soc., 2017, 139(28):9471-9474.

    21. [21]

      CAVKA J H, JAKOBSEN S, OLSBYE U, GUILLOU N, LAMBERTI C, BORDIGA S, LILLERUD K P. J. Am. Chem. Soc., 2008, 130(42):13850-13851.CAVKA J H, JAKOBSEN S, OLSBYE U, GUILLOU N, LAMBERTI C, BORDIGA S, LILLERUD K P. J. Am. Chem. Soc., 2008, 130(42):13850-13851.

    22. [22]

      GARIBAYA S J, COHEN S M. Chem. Commun., 2010, 46(41):7700-7702.GARIBAYA S J, COHEN S M. Chem. Commun., 2010, 46(41):7700-7702.

    23. [23]

      DONG M J, ZHAO M, OU S, ZOU C, WU C D. Angew. Chem., Int. Ed., 2014, 53(6):1575-1579.DONG M J, ZHAO M, OU S, ZOU C, WU C D. Angew. Chem., Int. Ed., 2014, 53(6):1575-1579.

    24. [24]

      CHEN D M, ZHANG N N, LIU C S, DU M. ACS Appl. Mater. Interfaces, 2017, 9(29):24671-24677.CHEN D M, ZHANG N N, LIU C S, DU M. ACS Appl. Mater. Interfaces, 2017, 9(29):24671-24677.

    25. [25]

      YU K H, WEI T X, LI Z J, LI J Y, WANG Z Y, DAI Z H. J. Am. Chem. Soc., 2020, 142(51):21267-21271.YU K H, WEI T X, LI Z J, LI J Y, WANG Z Y, DAI Z H. J. Am. Chem. Soc., 2020, 142(51):21267-21271.

    26. [26]

      WANG S Z, MCGUIRK C M, ROSS M B, WANG S Y, CHEN P C, XING H, LIU Y, MIRKIN C A. J. Am. Chem. Soc., 2017, 139(29):9827-9830.WANG S Z, MCGUIRK C M, ROSS M B, WANG S Y, CHEN P C, XING H, LIU Y, MIRKIN C A. J. Am. Chem. Soc., 2017, 139(29):9827-9830.

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  • 收稿日期:  2022-01-19
  • 修回日期:  2022-10-17
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