Citation: HUANG Xiao-Mei, DENG Xiang, XING Lang-Man, CHEN Wei, SUN Li, ZHU Xiao-Yu. An Electrochemiluminescence Sensor Based on Gold and Silver Bimetal Nanocluster and Ternary Carbon Nanosheets for Detection of Alpha Fetoprotein[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1567-1577. doi: 10.19756/j.issn.0253-3820.221029 shu

An Electrochemiluminescence Sensor Based on Gold and Silver Bimetal Nanocluster and Ternary Carbon Nanosheets for Detection of Alpha Fetoprotein

HUANG Xiao-Mei ^1,2,3,, ,
DENG Xiang ^1,2 ,
XING Lang-Man ^1,2 ,
CHEN Wei ¹ ,
SUN Li ¹ ,
ZHU Xiao-Yu ¹

1.
Department of Chemistry and Chemical Engineering, Sichuan University of Arts and Science, Dazhou 635000, China;
2.
Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province, Dazhou 635000, China;
3.
Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education, Zigong 643000, China

Corresponding author: HUANG Xiao-Mei, huangxm917@163.com
Received Date: 17 January 2022
Revised Date: 30 June 2022

Fund Project: Supported by the Scientific Research Fund of the Sichuan Provincial Science and Technology Department (No.2019YJ0307), the Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province (No.TSZW2005, TSZW2004), the Opening Project of Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education (No.LYJ1802), the Dazhou Municipal Science Project of Technology Bureau Application Foundation (No.18YYJC0002) and the National University Innovation and Entrepreneurship Training Program(No.202110644011).

Carbon-based transition metal oxide nanosheets metal-organic framework material(Pd NPs-Cu(Ⅱ)Co(Ⅱ)@C) were prepared by one-step room temperature process, pyrolysis in air, and in-situ reduction method. Then the Au-Ag NCs-TAEA-Pd NPs-Cu(Ⅱ)Co(Ⅱ)@C ternary luminescent composite nanomaterials were synthesized for the first time with gold-silver bimetallic nanoclusters (Au-Ag NCs) as luminophor, tri-(3-aminoethyl) amine (TAEA) as coreactant and Pd NPs-Cu(Ⅱ)Co(Ⅱ)@C as coreaction accelerator. A sandwich electrochemiluminescence (ECL) immunosensor was thus constructed by combining the ternary luminescent composite with alpha fetoprotein (AFP) secondary antibody as capture probe for ultra-sensitive detection of AFP. The ternary Au-Ag NCs-TAEA-Pd NPs-Cu(Ⅱ)Co(Ⅱ)@C luminescent nanocomposites had excellent ECL properties due to the dual catalytic synergy of intramolecular coreaction promoters and intramolecular coreactants, and the "silver effect" of bimetallic nanoclusters. Without any additional signal amplification strategy, the detection range of the ECL biosensor for AFP was 0.001-100 ng/mL, and the detection limit was as low as 0.3 pg/mL. Moreover, the proposed ECL immunosensor was expected to be applied to the diagnosis and bioanalysis of other biomolecules.
- Au-Ag bimetallic nanocluster,
- Metal-organic framework material,
- Alpha fetoprotein,
- Electrochemiluminescence,
- Coreaction accelerator
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