Citation: ZHANG Yu-Yu, HUANG Ya-Yue, ZENG Hui, YANG Tao, LUO Xi-Liang. Electrochemical Sensing Interface Based on Synergistic Antifouling of Polyethylene Glycol and Chondroitin Sulfate for Sensitive Detection of tlh Gene Segment of Vibrio Parahaemolyticus[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 1041-1047. doi: 10.19756/j.issn.0253-3820.221021 shu

Electrochemical Sensing Interface Based on Synergistic Antifouling of Polyethylene Glycol and Chondroitin Sulfate for Sensitive Detection of tlh Gene Segment of Vibrio Parahaemolyticus

ZHANG Yu-Yu ¹ ,
HUANG Ya-Yue ¹ ,
ZENG Hui ¹ ,
YANG Tao ^1,, ,
LUO Xi-Liang ^2,,

1.
School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China;
2.
School of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266061, China

Corresponding author: YANG Tao, LUO Xi-Liang,
Received Date: 12 January 2022
Revised Date: 29 April 2022

Fund Project: Supported by the National Natural Science Foundation of China (No.21675092), the Special Project on the Integration of Industry, Education and Research of Guangzhou (No.201604016008) and the Foshan Nanhai Economic and Technological Promotion Bureau Project (No.20177611071010008).

Foodborne pathogens are the main cause of seafood poisoning in the world, and the traditional electrochemical detection methods have always suffered from nonspecific adsorption and serious biological fouling in the practical complex samples. Therefore, it is urgent to develop antifouling and sensitive biosensor platforms for detecting foodborne pathogens in complex matrix. In this study, by integrating polyethylene glycol (PEG) with good hydrophilicity and chondroitin sulfate (CS) with excellent biocompatibility, an electrochemical DNA sensing interface for detection of the tlh gene segment of Vibrio parahaemolyticus based on synergistic antifouling of PEG and CS was constructed. Compared with sole poly(m-aminobenzoic acid) modified glassy carbon electrode (PABA/GCE), PEG/PABA/GCE and CS/PABA/GCE, CS/PEG/PABA/GCE exhibited many advantages such as excellent anti-protein (single protein and complex skim milk) adsorption performance and stability. Moreover, the sensing interface showed high sensitivity and satisfactory selectivity to the tlh gene segment in the range of 1.0×10^-20-1.0×10^-8 mol/L with a detection limit of 3.3×10^-21 mol/L.
- Electrochemical DNA sensor,
- Antifouling,
- Polyethylene glycol,
- Chondroitin sulfate,
- Vibrio parahaemolyticus
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