微量蛋白质组学样品预处理方法的研究进展

引用本文: 郑敏阳, 陆刚, 李洋, 张振宾, 赵玉芬. 微量蛋白质组学样品预处理方法的研究进展[J]. 分析化学, 2022, 50(9): 1279-1288. doi: 10.19756/j.issn.0253-3820.221004 shu

Citation: ZHENG Min-Yang, LU Gang, LI Yang, ZHANG Zhen-Bin, ZHAO Yu-Fen. Recent Development in Mass-limited Sample Preparation Methods for Proteomics[J]. Chinese Journal of Analytical Chemistry, 2022, 50(9): 1279-1288. doi: 10.19756/j.issn.0253-3820.221004 shu

微量蛋白质组学样品预处理方法的研究进展

宁波大学新药技术研究院, 宁波 315211

通讯作者: 张振宾,E-mail:zhangzhenbin@nbu.edu.cn; 赵玉芬,E-mail:zhaoyufen@nbu.edu.cn

基金项目:
宁波大学科研经费项目（No.215-432000282）、宁波市顶尖人才项目（No.215-432094250）和宁波大学王宽诚幸福基金项目资助。

摘要: 样品预处理方法对蛋白质组学分析方法的灵敏度和定量分析的准确性具有重要影响,在微量蛋白质组学样品分析中尤为重要。传统的蛋白质组样品预处理方法,如溶液内酶解法和滤膜辅助样品处理(FASP)方法等,处理样品时样品量损失较大,需要大量的起始样品(>20 μg)才能获得比较满意的蛋白质覆盖率,不适用于同一细胞亚型的少量细胞样品、临床穿刺样品以及单细胞等微量样品(<1 μg)的定性与定量分析。为了解决该问题,亟需发展针对亚微克甚至纳克级起始样品的蛋白质组学样品预处理方法。本文综述了固相萃取头法(In-stagetip)、固相增强一锅法(Single-pot solid-phase-enhanced sample preparation,SP3)、集成化的蛋白质组分析装置法(Integrated proteome analysis device,iPAD)、基于spintip的简单集成的蛋白质组学分析技术(Simple and integrated spintip-based proteomics technology,SISPROT)、全自动化样品处理(Fully automated sample treatment,FAST)和微型滤膜辅助法(Miniaturized filter-aided sample preparation,MICROFASP)等基于不同机理的微量蛋白质组学样品预处理方法的研究进展,并对其发展趋势进行了展望。

关键词:

English

Recent Development in Mass-limited Sample Preparation Methods for Proteomics

Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China

Corresponding author: ZHANG Zhen-Bin, ; ZHAO Yu-Fen,

Received Date: 03 January 2022
Revised Date: 25 May 2022
Fund Project:
Supported by the Research Funding of Ningbo University (No.215-432000282), the Ningbo Top Talent Project (No.215-432094250) and the K.C. Wong Magna Fund in Ningbo University.

Abstract: Sample preparation methods determine the sensitivity and quantitative accuracy of proteomics analysis, especially for mass-limited proteomics sample. Traditional preparation methods of proteomic sample, such as in-solution digestion and filter aided sample preparation (FASP) method, generally require large amount of starting materials due to the significant sample loss, and thus is not suitable for the qualitative and quantitative analysis of mass-limited samples, such as small number of subcellular cells, biopsy samples, single cells, et al. To overcome this problem, it is urgent to develop sample preparation methods for microgram or even nanogram of starting materials. This paper reviewed various mass-limited sample preparation methods that based on different mechanisms, such as in-StageTip, single-pot solid-phase-enhanced sample preparation (SP3), integrated proteome analysis device (iPAD), simple and integrated spintip-based proteomics technology (SISPROT), fully automated sample treatment (FAST), miniaturized filter-aided sample preparation (MICROFASP) and so on. The development trends in the future were also envisioned.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

微量蛋白质组学样品预处理方法的研究进展

通讯作者: 张振宾,E-mail:zhangzhenbin@nbu.edu.cn; 赵玉芬,E-mail:zhaoyufen@nbu.edu.cn

English

Recent Development in Mass-limited Sample Preparation Methods for Proteomics

Corresponding author: ZHANG Zhen-Bin, ; ZHAO Yu-Fen,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

微量蛋白质组学样品预处理方法的研究进展

通讯作者: 张振宾,E-mail:zhangzhenbin@nbu.edu.cn; 赵玉芬,E-mail:zhaoyufen@nbu.edu.cn

English

Recent Development in Mass-limited Sample Preparation Methods for Proteomics

Corresponding author: ZHANG Zhen-Bin, ; ZHAO Yu-Fen,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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