基于β-二酮类铕(Ⅲ)配合物的一氧化氮荧光探针的设计、合成及应用研究

闵盼 郑德泽 车婉萌 邢月 吴晶

引用本文: 闵盼, 郑德泽, 车婉萌, 邢月, 吴晶. 基于β-二酮类铕(Ⅲ)配合物的一氧化氮荧光探针的设计、合成及应用研究[J]. 分析化学, 2021, 49(10): 1704-1712. doi: 10.19756/j.issn.0253-3820.211233 shu
Citation:  MIN Pan,  ZHENG De-Ze,  CHE Wan-Meng,  XING Yue,  WU Jing. Design and Synthesis of A β-Diketonate-Europium(Ⅲ) Complex-based Fluorescent Probe for Nitric Oxide Detection and Its Application in Bioimaging[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1704-1712. doi: 10.19756/j.issn.0253-3820.211233 shu

基于β-二酮类铕(Ⅲ)配合物的一氧化氮荧光探针的设计、合成及应用研究

    通讯作者: 吴晶,E-mail:wujing@lnnu.edu.cn
  • 基金项目:

    2019年国家大学生创新训练计划项目(No.201910165118)资助。

摘要: 以含一氧化氮(NO)识别基团邻二氨基苯基的联三吡啶衍生物4-(4-[2,2':6',2″]三联吡啶-4'-基-苯氧基)-苯-1,2-二胺(TPBD)为配位体,β-二酮类化合物5-(1',1″-二苯基-4'-基)-1,1,1,2,2,3,3-七氟-4,6-己二酮(DHH)为共配体,设计并合成了一种用于检测NO的β-二酮类铕(Ⅲ)配合物荧光探针[Eu (DHH)3(TPBD)],采用质谱和元素分析确证其结构。光谱研究结果表明,探针[Eu (DHH)3(TPBD)]本身仅发出微弱的光,与NO反应后荧光强度增强近10倍,定量检测NO的线性浓度范围为0.017~0.83 μmol/L,检出限为7.4 nmol/L。探针[Eu (DHH)3(TPBD)]对几种常见的活性氧/氮物种基本上不响应,对NO显示出良好的选择性。荧光成像实验表明,探针[Eu (DHH)3(TPBD)]具有低细胞毒性和线粒体靶向定位性能,并成功实现对RAW 264.7活细胞和斑马鱼体内的NO的时间分辨荧光成像检测。

English


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  • 收稿日期:  2021-03-19
  • 修回日期:  2021-08-03
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