反相色谱分离和葡萄糖苯脎法测定人唾液中白蛋白的糖化水平

蔡沅君 关升 晏国全 张祥民

引用本文: 蔡沅君, 关升, 晏国全, 张祥民. 反相色谱分离和葡萄糖苯脎法测定人唾液中白蛋白的糖化水平[J]. 分析化学, 2021, 49(9): 1540-1545. doi: 10.19756/j.issn.0253-3820.211200 shu
Citation:  CAI Yuan-Jun,  GUAN Sheng,  YAN Guo-Quan,  ZHANG Xiang-Min. Chromatographic Separation and Quantitative Analysis of Glycated Albumin in Human Saliva by Glucose Phenylosazone Spectrophotometric Assay[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1540-1545. doi: 10.19756/j.issn.0253-3820.211200 shu

反相色谱分离和葡萄糖苯脎法测定人唾液中白蛋白的糖化水平

    通讯作者: 晏国全,E-mail:yanguoquan@fudan.edu.cn; 张祥民,E-mail:xmzhang@fudan.edu.cn
  • 基金项目:

    国家重点研发计划项目(No.2017YFA0505003)和国家自然科学基金项目(No.21775027)资助。

摘要: 采用一种无创取样方法实现了人体内糖化白蛋白的定量分析。以唾液为测试样品,首先通过超滤分离出唾液中分子量大于10 kD的蛋白分子,再经过反相色谱对唾液中的白蛋白进行进一步分离提纯,其中36.0~37.0 min的馏分经质谱鉴定为人血清白蛋白。通过葡萄糖苯脎分光光度法对唾液馏分中的白蛋白进行定量分析,并与临床中使用的白蛋白定量分析方法进行比较。结果表明,对于健康样品,5次平行实验的平均糖化比例为10.9%,相对标准偏差为11.3%。本方法可定量分析健康人唾液中的糖化白蛋白,有望用于临床糖尿病初步筛查。

English


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  • 收稿日期:  2021-03-16
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