基于植物酯酶-Cu3(PO4)2杂化纳米花的酶抑制型方法检测敌敌畏残留

曲林姣 王金鑫 姜磊 马洪超 杨丽敏

引用本文: 曲林姣, 王金鑫, 姜磊, 马洪超, 杨丽敏. 基于植物酯酶-Cu3(PO4)2杂化纳米花的酶抑制型方法检测敌敌畏残留[J]. 分析化学, 2021, 49(9): 1506-1514. doi: 10.19756/j.issn.0253-3820.211076 shu
Citation:  QU Lin-Jiao,  WANG Jin-Xin,  JIANG Lei,  MA Hong-Chao,  YANG Li-Min. Detection of Dichlorvos Residue by Enzyme Inhibition Method Based on Plant Esterase-Cu3(PO4)2 Hybrid Nanoflowers[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1506-1514. doi: 10.19756/j.issn.0253-3820.211076 shu

基于植物酯酶-Cu3(PO4)2杂化纳米花的酶抑制型方法检测敌敌畏残留

    通讯作者: 杨丽敏,E-mail:yanglimin@upc.edu.cn
  • 基金项目:

    国家自然科学基金项目(No.31871878)和中国石油大学(华东)大学生创新创业训练计划项目(No.202003022)资助。

摘要: 制备了植物酯酶-Cu3(PO42杂化纳米花,实现了植物酯酶的固定,保持了其水解酶活性,而且引入的磷酸铜骨架不仅具有支撑作用,还具有过氧化物酶模拟酶活性。与游离酶不同,杂化纳米花具有较高的稳定性,在储存30天后,其水解酶活性和过氧化物酶模拟酶活性都维持在80%以上;同时,分层纳米结构具有较大的比表面积,有利于酶与底物之间的反应。基于此杂化纳米花的双酶活性,将其运用于1-萘酚偶联双酶反应的酶抑制型检测方法,实现了有机磷农药敌敌畏的灵敏检测。本方法对敌敌畏的检出限(LOD)为0.26 pmol/L,实际蔬菜水果样品中敌敌畏的回收率为96.8%~107.4%。

English


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  • 收稿日期:  2021-01-27
  • 修回日期:  2021-05-13
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