Citation: QU Lin-Jiao, WANG Jin-Xin, JIANG Lei, MA Hong-Chao, YANG Li-Min. Detection of Dichlorvos Residue by Enzyme Inhibition Method Based on Plant Esterase-Cu₃(PO₄)₂ Hybrid Nanoflowers[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1506-1514. doi: 10.19756/j.issn.0253-3820.211076 shu

Detection of Dichlorvos Residue by Enzyme Inhibition Method Based on Plant Esterase-Cu₃(PO₄)₂ Hybrid Nanoflowers

Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, China

Corresponding author: YANG Li-Min, yanglimin@upc.edu.cn
Received Date: 27 January 2021
Revised Date: 13 May 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.31871878) and the China University of Petroleum (East China) College Students' Innovation and Entrepreneurship Training Program (No.202003022).

Plant esterase-Cu₃(PO₄)₂ hybrid nanoflowers were prepared, which not only achieved the immobilization of plant esterase and maintained its hydrolase activity, but also skillfully used copper phosphate skeleton. The copper phosphate skeleton not only had the supporting role, but also had the peroxidase-like activity. Different from free enzymes, hybrid nanoflowers showed higher stability, and their hydrolase activity and peroxidase-like activity maintained above 80% after storage for 30 days. Meanwhile, the layered nanostructures had larger specific surface area, which facilitated the reaction between enzyme and substrate. Due to the dual enzyme activity, the hybrid nanoflowers were applied to the enzyme inhibition-based colorimetric assay of 1-naphthol-linked bienzymatic reaction for the sensitive detection of organophosphorus pesticides. The limit of detection (LOD) for dichlorvos was as low as 0.26 pmol/L. The recoveries of dichlorvos in vegetables and fruits were 96.8%-107.4%.
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Detection of Dichlorvos Residue by Enzyme Inhibition Method Based on Plant Esterase-Cu3(PO4)2 Hybrid Nanoflowers

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通讯作者: 陈斌, bchen63@163.com

Detection of Dichlorvos Residue by Enzyme Inhibition Method Based on Plant Esterase-Cu₃(PO₄)₂ Hybrid Nanoflowers