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基于目标物调控原位生成量子点的杀扑磷荧光传感新方法研究

王雪梅 ,  赵岁欣 ,  李海银 ,  李峰

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引用本文: 王雪梅, 赵岁欣, 李海银, 李峰. 基于目标物调控原位生成量子点的杀扑磷荧光传感新方法研究[J]. 分析化学, 2022, 50(3): 375-383. doi: 10.19756/j.issn.0253-3820.210847 shu
Citation:  WANG Xue-Mei,  ZHAO Sui-Xin,  LI Hai-Yin,  LI Feng. Target-controlled In-Situ Formation of Quantum Dots for Fluorescence Sensing of Methidathion[J]. Chinese Journal of Analytical Chemistry, 2022, 50(3): 375-383. doi: 10.19756/j.issn.0253-3820.210847 shu

基于目标物调控原位生成量子点的杀扑磷荧光传感新方法研究

  • 青岛农业大学化学与药学院, 青岛 266109

    • 通讯作者: 李海银,E-mail:lihaiyin@qau.edu.cn; 李峰,E-mail:lifeng@qau.edu.cn
  • 基金项目:

    国家自然科学基金项目（Nos.21775082，22076090）资助。

摘要: 基于目标物调控原位生成荧光物质，建立了硫化镉量子点（CdS QDs）介导的荧光传感器，实现了大米中有机磷农药杀扑磷的高灵敏、高选择性分析检测。乙酰胆碱酯酶（AChE）催化水解硫代乙酰胆碱（ATCh）生成硫代胆碱（TCh），其作为稳定剂诱导原位生成大量CdS QDs，体系荧光信号增强。当目标农药杀扑磷存在时，AChE活性被抑制，无法有效催化水解ATCh生成TCh，CdS QDs生成量减少，致使检测体系的荧光信号降低。基于目标物加入前后检测体系荧光强度的变化，实现了杀扑磷的高灵敏检测，检出限为0.024 ng/mL（S/N=3）。利用本方法检测大米样品中杀扑磷的含量，加标回收率在96.7%~102.4%之间。本研究为食品中有机磷农药残留的灵敏与精准检测提供了新思路。

English


    1. [1]

      LUO D Q, HUANG X H, LIU B Y, ZOU W Y, WU Y G. J. Agric. Food Chem., 2021, 69(11):3537-3547.LUO D Q, HUANG X H, LIU B Y, ZOU W Y, WU Y G. J. Agric. Food Chem., 2021, 69(11):3537-3547.

    2. [2]

      CAI Y, FANG J K, WANG B F, ZHANG F S, SHAO G, LIU Y J. Sens. Actuators, B, 2019, 292:156-163.CAI Y, FANG J K, WANG B F, ZHANG F S, SHAO G, LIU Y J. Sens. Actuators, B, 2019, 292:156-163.

    3. [3]

      LIU B Y, TANG Y, YANG Y X, WU Y G. Food Control, 2021, 129:108208.LIU B Y, TANG Y, YANG Y X, WU Y G. Food Control, 2021, 129:108208.

    4. [4]

      ZHOU J W, ZOU X M, SONG S H, CHEN G H. J. Agric. Food Chem., 2018, 66(6):1307-1319.ZHOU J W, ZOU X M, SONG S H, CHEN G H. J. Agric. Food Chem., 2018, 66(6):1307-1319.

    5. [5]

      CHEN J L, CHEN X J, HUANG Q Y, LI W L, YU Q X, ZHU L J, ZHU T W, LIU S W, CHI Z G. ACS Appl. Mater. Interfaces, 2019, 11(36):32689-32696.CHEN J L, CHEN X J, HUANG Q Y, LI W L, YU Q X, ZHU L J, ZHU T W, LIU S W, CHI Z G. ACS Appl. Mater. Interfaces, 2019, 11(36):32689-32696.

    6. [6]

      GARAI-IBABE G, SAA L, PAVLOV V. Analyst, 2014, 139(1):280-284.GARAI-IBABE G, SAA L, PAVLOV V. Analyst, 2014, 139(1):280-284.

    7. [7]

      HE X J, DENG Z A, XU W, LI Y H, XU C C, CHEN H, SHEN J L. Sens. Actuators, B, 2020, 321:128450.HE X J, DENG Z A, XU W, LI Y H, XU C C, CHEN H, SHEN J L. Sens. Actuators, B, 2020, 321:128450.

    8. [8]

      LU C, CHEN X. ACS Nano, 2021, 15(12):18777-18793.LU C, CHEN X. ACS Nano, 2021, 15(12):18777-18793.

    9. [9]

      SUN J H, ZHOU F, HU H, LI N, XIA M M, WANG L, WANG X Y, WANG G F. Anal. Chem., 2020, 92(8):6136-6143.SUN J H, ZHOU F, HU H, LI N, XIA M M, WANG L, WANG X Y, WANG G F. Anal. Chem., 2020, 92(8):6136-6143.

    10. [10]

      AHMAD I, ZHOU Z, LI H Y, ZANG S Q. Sens. Actuators, B, 2020, 304:127379.AHMAD I, ZHOU Z, LI H Y, ZANG S Q. Sens. Actuators, B, 2020, 304:127379.

    11. [11]

      ZHANG J, ZHOU W D, ZHAI L J, NIU X Y, HU T P. CrystEngComm, 2020, 22(6):1050-1056.ZHANG J, ZHOU W D, ZHAI L J, NIU X Y, HU T P. CrystEngComm, 2020, 22(6):1050-1056.

    12. [12]

      YUAN X Y, ZHANG D W, ZHU X C, LIU H L, SUN B G. Food Chem., 2021, 342:128299.YUAN X Y, ZHANG D W, ZHU X C, LIU H L, SUN B G. Food Chem., 2021, 342:128299.

    13. [13]

      HE Y, HU F X, ZHAO J W, YANG G M, ZHANG Y Y, CHEN S H, YUAN R. Anal. Chem., 2021, 93(25):8783-8790.HE Y, HU F X, ZHAO J W, YANG G M, ZHANG Y Y, CHEN S H, YUAN R. Anal. Chem., 2021, 93(25):8783-8790.

    14. [14]

      HE W M, ZHOU Z, HAN Z, LI S, ZHOU Z, MA L F, ZANG S Q. Angew. Chem., Int. Ed., 2021, 60(15):8505-8509.HE W M, ZHOU Z, HAN Z, LI S, ZHOU Z, MA L F, ZANG S Q. Angew. Chem., Int. Ed., 2021, 60(15):8505-8509.

    15. [15]

      CAO Z Y, SHU Y F, QIN H Y, SU B, PENG X G. ACS Cent. Sci., 2020, 6(7):1129-1137.CAO Z Y, SHU Y F, QIN H Y, SU B, PENG X G. ACS Cent. Sci., 2020, 6(7):1129-1137.

    16. [16]

      ZHAO Q, LU D, ZHANG G Y, ZHANG D, SHI X B. Talanta, 2021, 223(Pt 1):121722.ZHAO Q, LU D, ZHANG G Y, ZHANG D, SHI X B. Talanta, 2021, 223(Pt 1):121722.

    17. [17]

      CHEN Z P, WANG H, ZHANG Z Y, CHEN L X. Anal. Chem., 2019, 91(2):1254-1259.CHEN Z P, WANG H, ZHANG Z Y, CHEN L X. Anal. Chem., 2019, 91(2):1254-1259.

    18. [18]

      CHEN X J, HE J H, TAN G Y, LIANG J, HOU Y X, WANG M, WANG B M. Food Chem., 2019, 291:132-138.CHEN X J, HE J H, TAN G Y, LIANG J, HOU Y X, WANG M, WANG B M. Food Chem., 2019, 291:132-138.

    19. [19]

      JIA M, CHEN S, SHI T T, LI C Y, WANG Y P, ZHANG H Y. Food Chem., 2021, 344:128602.JIA M, CHEN S, SHI T T, LI C Y, WANG Y P, ZHANG H Y. Food Chem., 2021, 344:128602.

    20. [20]

      DONG C Y, SHI H X, HAN Y R, YANG Y Y, WANG R X, MEN J Y. Eur. Polym. J., 2021, 145:110231.DONG C Y, SHI H X, HAN Y R, YANG Y Y, WANG R X, MEN J Y. Eur. Polym. J., 2021, 145:110231.

    21. [21]

      JIA M F, ZHANG Z, LI J H, MA X, CHEN L X, YANG X B. TrAC-Trends Anal. Chem., 2018, 106:190-201.JIA M F, ZHANG Z, LI J H, MA X, CHEN L X, YANG X B. TrAC-Trends Anal. Chem., 2018, 106:190-201.

    22. [22]

      MADIKIZELA L M, TAVENGWA N T, TUTU H, CHIMUKA L. Trends Environ. Anal. Chem., 2018, 17:14-22.MADIKIZELA L M, TAVENGWA N T, TUTU H, CHIMUKA L. Trends Environ. Anal. Chem., 2018, 17:14-22.

    23. [23]

      KORRAM J, DEWANGAN L, KARBHAL I, NAGWANSHI R, VAISHANAV S K, GHOSH K K, SATNAMI M L. RSC Adv., 2020, 10(41):24190-24202.KORRAM J, DEWANGAN L, KARBHAL I, NAGWANSHI R, VAISHANAV S K, GHOSH K K, SATNAMI M L. RSC Adv., 2020, 10(41):24190-24202.

    24. [24]

      SINGH A P, BALAYAN S, HOODA V, SARIN R K, CHAUHAN N. Int. J. Biol. Macromol., 2020, 164:3943-3952.SINGH A P, BALAYAN S, HOODA V, SARIN R K, CHAUHAN N. Int. J. Biol. Macromol., 2020, 164:3943-3952.

    25. [25]

      LV B J, WEI M, LIU Y J, LIU X, WEI W, LIU S Q. Microchim. Acta, 2016, 183(11):2941-2948.LV B J, WEI M, LIU Y J, LIU X, WEI W, LIU S Q. Microchim. Acta, 2016, 183(11):2941-2948.

    26. [26]

      WANG D, LIN B X, CAO Y J, GUO M L, YU Y. J. Agric. Food Chem., 2016, 64(30):6042-6050.WANG D, LIN B X, CAO Y J, GUO M L, YU Y. J. Agric. Food Chem., 2016, 64(30):6042-6050.

    27. [27]

      WANG J L, XIA Q, ZHANG A P, HU X Y, LIN C M. J. Zhejiang Univ. Sci. B, 2012, 13(4):267-273.WANG J L, XIA Q, ZHANG A P, HU X Y, LIN C M. J. Zhejiang Univ. Sci. B, 2012, 13(4):267-273.

    28. [28]

      HUANG X B, LV M, MA Q J, ZHANG Y Y, XU H. J. Agric. Food Chem., 2021, 69(48):14488-14500.HUANG X B, LV M, MA Q J, ZHANG Y Y, XU H. J. Agric. Food Chem., 2021, 69(48):14488-14500.

    29. [29]

      GHOTO S A, KHUHAWAR M Y, JAHANGIR T M, MANGI J U D. J. Nanostruct. Chem., 2019, 9(2):77-93.GHOTO S A, KHUHAWAR M Y, JAHANGIR T M, MANGI J U D. J. Nanostruct. Chem., 2019, 9(2):77-93.

    30. [30]

      CHEN Q, SUN Y D, LIU S J, ZHANG J, ZHANG C, JIANG H, HAN X Y, HE L F, WANG S H, ZHANG K. Sens. Actuators, B, 2021, 344:130278.CHEN Q, SUN Y D, LIU S J, ZHANG J, ZHANG C, JIANG H, HAN X Y, HE L F, WANG S H, ZHANG K. Sens. Actuators, B, 2021, 344:130278.

    31. [31]

      FAHIMI-KASHANI N, HORMOZI-NEZHAD M R. Anal. Chem., 2016, 88(16):8099-8106.FAHIMI-KASHANI N, HORMOZI-NEZHAD M R. Anal. Chem., 2016, 88(16):8099-8106.

    32. [32]

      WANG J Y, ZHANG J Y, WANG J, FANG G Z, LIU J F, WANG S. J. Hazard. Mater., 2020, 389:122074.WANG J Y, ZHANG J Y, WANG J, FANG G Z, LIU J F, WANG S. J. Hazard. Mater., 2020, 389:122074.

    33. [33]

      GUAN J P, YANG J, ZHANG Y, ZHANG X X, DENG H J, XU J, WANG J Y, YUAN M S. Talanta, 2021, 224:121834.GUAN J P, YANG J, ZHANG Y, ZHANG X X, DENG H J, XU J, WANG J Y, YUAN M S. Talanta, 2021, 224:121834.

    34. [34]

      GUO W Y, FU Y X, LIU S Y, MEI L C, SUN Y, YIN J, YANG W C, YANG G F. Anal. Chem., 2021, 93(18):7079-7085.GUO W Y, FU Y X, LIU S Y, MEI L C, SUN Y, YIN J, YANG W C, YANG G F. Anal. Chem., 2021, 93(18):7079-7085.

    35. [35]

      FAN Y, LIU L, SUN D L, LAN H Y, FU H Y, YANG T M, SHE Y B, NI C. Anal. Chim. Acta, 2016, 916:84-91.FAN Y, LIU L, SUN D L, LAN H Y, FU H Y, YANG T M, SHE Y B, NI C. Anal. Chim. Acta, 2016, 916:84-91.

    36. [36]

      CHEN J L, LI M Q, ZHOU X Q, XIE A L, CAI Z W, FU C L, PENG Y M, ZHANG H, LIU L H. J. Agric. Food Chem., 2021, 69(46):13942-13952.CHEN J L, LI M Q, ZHOU X Q, XIE A L, CAI Z W, FU C L, PENG Y M, ZHANG H, LIU L H. J. Agric. Food Chem., 2021, 69(46):13942-13952.

    37. [37]

      ZHANG J, WU Q Q, ZHONG Y R, WANG Z, HE Z Z, ZHANG Y Q, WANG M H. J. Agric. Food Chem., 2021, 69(45):13416-13424.ZHANG J, WU Q Q, ZHONG Y R, WANG Z, HE Z Z, ZHANG Y Q, WANG M H. J. Agric. Food Chem., 2021, 69(45):13416-13424.

    38. [38]

      YAN X, LI H X, HU T Y, SU X G. Biosens. Bioelectron., 2017, 91:232-237.YAN X, LI H X, HU T Y, SU X G. Biosens. Bioelectron., 2017, 91:232-237.

    39. [39]

      CUI H F, ZHANG T T, LV Q Y, SONG X, ZHAI X J, WANG G G. Biosens. Bioelectron., 2019, 141:111452.CUI H F, ZHANG T T, LV Q Y, SONG X, ZHAI X J, WANG G G. Biosens. Bioelectron., 2019, 141:111452.

    40. [40]

      MONDOL M M H, JHUNG S H. Chem. Eng. J., 2021, 421(Part 1):129688.MONDOL M M H, JHUNG S H. Chem. Eng. J., 2021, 421(Part 1):129688.

    41. [41]

      ALDEWACHI H, CHALATI T, WOODROOFE M N, BRICKLEBANK N, SHARRACK B, GARDINER P. Nanoscale, 2017, 10(1):18-33.ALDEWACHI H, CHALATI T, WOODROOFE M N, BRICKLEBANK N, SHARRACK B, GARDINER P. Nanoscale, 2017, 10(1):18-33.

    42. [42]

      LIN B X, YAN Y, GUO M L, CAO Y J, YU Y, ZHANG T Y, HUANG Y, WU D. Food Chem., 2018, 245:1176-1182.LIN B X, YAN Y, GUO M L, CAO Y J, YU Y, ZHANG T Y, HUANG Y, WU D. Food Chem., 2018, 245:1176-1182.

    43. [43]

      LIU M L, WEI J Y, WANG Y, OUYANG H, FU Z F. Talanta, 2019, 195:706-712.LIU M L, WEI J Y, WANG Y, OUYANG H, FU Z F. Talanta, 2019, 195:706-712.

    44. [44]

      SAHOO D, MANDAL A, MITRA T, CHAKRABORTY K, BARDHAN M, DASGUPTA A K. J. Agric. Food Chem., 2018, 66(2):414-423.SAHOO D, MANDAL A, MITRA T, CHAKRABORTY K, BARDHAN M, DASGUPTA A K. J. Agric. Food Chem., 2018, 66(2):414-423.

    45. [45]

      CHEN Q D, FUNG Y. Electrophoresis, 2010, 31(18):3107-3114.CHEN Q D, FUNG Y. Electrophoresis, 2010, 31(18):3107-3114.

    46. [46]

      WANG P Y, LI H H, HASSAN M M, GUO Z M, ZHANG Z Z, CHEN Q. J. Agric. Food Chem., 2019, 67(14):4071-4079.WANG P Y, LI H H, HASSAN M M, GUO Z M, ZHANG Z Z, CHEN Q. J. Agric. Food Chem., 2019, 67(14):4071-4079.

    47. [47]

      DONG J J, YANG H T, LI Y, LIU A R, WEI W, LIU S Q. Anal. Chim. Acta, 2020, 1131:102-108.DONG J J, YANG H T, LI Y, LIU A R, WEI W, LIU S Q. Anal. Chim. Acta, 2020, 1131:102-108.

    48. [48]

      HONG C Y, YE S S, DAI C Y, WU C Y, CHEN L L, HUANG Z Y. Anal. Bioanal. Chem., 2020, 412(29):8177-8184.HONG C Y, YE S S, DAI C Y, WU C Y, CHEN L L, HUANG Z Y. Anal. Bioanal. Chem., 2020, 412(29):8177-8184.

    49. [49]

      WANG X D, YANG Y Y, DONG J, BEI F, AI S Y. Sens. Actuators, B, 2014, 204:119-124.WANG X D, YANG Y Y, DONG J, BEI F, AI S Y. Sens. Actuators, B, 2014, 204:119-124.

    50. [50]

      GB 2763-2016. Maximum Residue Limits for Pesticides in Food. National Standards of the People's Republic of China. 食品中农药最大残留限量. 中华人民共和国国家标准. GB 2763-2016.

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  • 收稿日期:  2021-11-18
  • 修回日期:  2021-12-09
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