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基于目标物调控原位生成量子点的杀扑磷荧光传感新方法研究

王雪梅 ,  赵岁欣 ,  李海银 ,  李峰

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引用本文: 王雪梅, 赵岁欣, 李海银, 李峰. 基于目标物调控原位生成量子点的杀扑磷荧光传感新方法研究[J]. 分析化学, 2022, 50(3): 375-383. doi: 10.19756/j.issn.0253-3820.210847 shu
Citation:  WANG Xue-Mei,  ZHAO Sui-Xin,  LI Hai-Yin,  LI Feng. Target-controlled In-Situ Formation of Quantum Dots for Fluorescence Sensing of Methidathion[J]. Chinese Journal of Analytical Chemistry, 2022, 50(3): 375-383. doi: 10.19756/j.issn.0253-3820.210847 shu

基于目标物调控原位生成量子点的杀扑磷荧光传感新方法研究

  • 青岛农业大学化学与药学院, 青岛 266109

    • 通讯作者: 李海银,E-mail:lihaiyin@qau.edu.cn; 李峰,E-mail:lifeng@qau.edu.cn
  • 基金项目:

    国家自然科学基金项目（Nos.21775082，22076090）资助。

摘要: 基于目标物调控原位生成荧光物质，建立了硫化镉量子点（CdS QDs）介导的荧光传感器，实现了大米中有机磷农药杀扑磷的高灵敏、高选择性分析检测。乙酰胆碱酯酶（AChE）催化水解硫代乙酰胆碱（ATCh）生成硫代胆碱（TCh），其作为稳定剂诱导原位生成大量CdS QDs，体系荧光信号增强。当目标农药杀扑磷存在时，AChE活性被抑制，无法有效催化水解ATCh生成TCh，CdS QDs生成量减少，致使检测体系的荧光信号降低。基于目标物加入前后检测体系荧光强度的变化，实现了杀扑磷的高灵敏检测，检出限为0.024 ng/mL（S/N=3）。利用本方法检测大米样品中杀扑磷的含量，加标回收率在96.7%~102.4%之间。本研究为食品中有机磷农药残留的灵敏与精准检测提供了新思路。

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  • 收稿日期:  2021-11-18
  • 修回日期:  2021-12-09
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