Citation: HE Mao-Fang, YAO Shu-Ting, ZHANG Yu-Die, WANG Xin, YANG Da-Long, WANG Qi-Rong, QIN Bei. Preparation of Hyperbranched Boronate Affinity Adsorbent with Improved Binding Strength and Its Application in Enrichment of Catecholamines[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(5): 739-746. doi: 10.19756/j.issn.0253-3820.210804 shu

Preparation of Hyperbranched Boronate Affinity Adsorbent with Improved Binding Strength and Its Application in Enrichment of Catecholamines

Institute of Drug Research, College of Pharmacy, Xi'an Medical University, Xi'an 710021, China

Corresponding author: QIN Bei, qinbei0526@163.com
Received Date: 21 October 2021
Revised Date: 15 January 2022

Fund Project: Supported by the Shaanxi Provincial Department of Science and Technology Key R&D Program (No.2021ZDLSF03-05), the Shaanxi Provincial Department of Science and Technology Natural Science Basic Research Program (No.2021JQ-773), the Shaanxi Provincial Department of Education Natural Science Basic Research Program (No.21JK0894) and the Key Pharmacy Discipline of Xi'an Medical University (Xi'an Medical University[2019]96).

A kind of hyperbranched boronate affinity adsorbent was prepared by combination of diboronic acid ligand with polyethyleneimine (PEI) on Fe₃O₄ microspheres. The optimal adsorption capacity and selectivity was obtained when PEI₁₈₀₀ was employed. The binding affinity of the adsorbent with biomolecules with different couples of cis-diols was investigated. It was shown that the dissociation constant (K_d) between the adsorbent and adenosine was 10^-4 mol/L. Compared with previous reports, the binding affinity was improved by 10 times. More interestingly, due to the synergistic binding of boronic acid groups with two or three couples of cis-diols, K_d between the adsorbent and chlorogenic acid, rosmarinic acid, salvianolic acid B and hyperoside was 10^-5 mol/L. Compared with adenosine, catechol and dopamine, the binding affinity was further improved by 10 times. Based on the strong binding strength, the adsorbent was applied in the enrichment of catecholamines from human urine and rat serum with high specificity, recovery and sensitivity. The adsorbent was expected to be further applied in the enrichment of nucleosides and glycoproteins in biological samples.
- Boronate affinity,
- Bisdentate boronate ligand,
- Synergistic binding,
- Binding strength,
- Catecholamines
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