树枝状高亲和性硼酸配体吸附剂的制备及其对儿茶酚胺的分离富集

贺茂芳 姚淑婷 张雨蝶 王欣 杨大龙 王起荣 秦蓓

引用本文: 贺茂芳, 姚淑婷, 张雨蝶, 王欣, 杨大龙, 王起荣, 秦蓓. 树枝状高亲和性硼酸配体吸附剂的制备及其对儿茶酚胺的分离富集[J]. 分析化学, 2022, 50(5): 739-746. doi: 10.19756/j.issn.0253-3820.210804 shu
Citation:  HE Mao-Fang,  YAO Shu-Ting,  ZHANG Yu-Die,  WANG Xin,  YANG Da-Long,  WANG Qi-Rong,  QIN Bei. Preparation of Hyperbranched Boronate Affinity Adsorbent with Improved Binding Strength and Its Application in Enrichment of Catecholamines[J]. Chinese Journal of Analytical Chemistry, 2022, 50(5): 739-746. doi: 10.19756/j.issn.0253-3820.210804 shu

树枝状高亲和性硼酸配体吸附剂的制备及其对儿茶酚胺的分离富集

    通讯作者: 秦蓓,E-mail:qinbei0526@163.com
  • 基金项目:

    陕西省科技厅重点研发计划项目(No.2021ZDLSF03-05)、陕西省科技厅自然科学基础研究计划项目(No.2021JQ-773)、陕西省教育厅科研计划项目(No.21JK0894)和西安医学院校级重点药学学科项目(No.西医发[2019]96号)资助。

摘要: 以Fe3O4为基质,将分子内含有两个硼酸基的二齿配体修饰在聚乙烯亚胺(PEI)的末端,制备了树枝状二齿硼酸配体磁性纳米吸附剂。研究表明,当PEI的分子量为1800时,制备的吸附剂具有最佳的吸附量和选择性。以含有不同顺式二羟基数的化合物为对象,研究了吸附剂的亲和力。结果表明,吸附剂与腺苷、邻苯二酚和多巴胺的解离常数(Kd)为10-4 mol/L;同时,由于高密度的硼酸基可与2对或3对顺式二羟基发生"协同吸附",此吸附剂与绿原酸、迷迭香酸、丹酚酸B和金丝桃苷的Kd为10-5 mol/L,相比于腺苷、邻苯二酚和多巴胺,其亲和力提高了10倍。将此吸附剂应用于人尿液和大鼠血清中儿茶酚胺(多巴胺、肾上腺素、去甲肾上腺素)的分离富集,结合高效液相色谱-荧光法检测,显示了良好的选择性、灵敏度和回收率。此吸附剂有望应用于核苷、糖蛋白等生物分子的分离富集。

English


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  • 收稿日期:  2021-10-21
  • 修回日期:  2022-01-15
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