Citation: JIAO Ting, WEN Hao-Xiang, LI Zhong-Ping. Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(2): 235-243. doi: 10.19756/j.issn.0253-3820.210770 shu

Selective Detection of Doxorubicin Hydrochloride Based on Fluorescence Quenching of Copper Nanoclusters

1.
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
2.
Institute of Environmental Sciences, Shanxi University, Taiyuan 030006, China;
3.
School of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030024, China

Corresponding author: LI Zhong-Ping, zl104@sxu.edu.cn
Received Date: 29 September 2021
Revised Date: 3 December 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21575083), the Program for the Innovative Teams of Jinzhong University (No.jzxycxtd2019007) and the Hundred Talents Program of the Twelfth Batch Shanxi Province (No.128,129)

A kind of novel copper nanoclusters (PEI@CuNCs) with sizes of about 2.4 nm was prepared using polyethyleneimine (PEI) as stabilizer and ascorbic acid as reducing agent, which could be used as probe to detect doxorubicin hydrochloride (DOX). The prepared PEI@CuNCs showed good stability and resistance to photobleaching, and their maximal excitation and emission wavelengths were 430 and 518 nm, respectively. The surface functional groups of PEI@CuNCs were characterized by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. DOX could quench the fluorescence of PEI@CuNCs, on the basis of this, a fluorescence method for detection of DOX was established. Under the optimal conditions, in the DOX concentration range of 1-145 μmol/L, a good linear relationship between the concentration of DOX and F/F₀ was observed, with a detection limit of 0.032 μmol/L (S/N=3). In the presence of other antibiotics, this method had high selectivity to DOX. The fluorescence quenching of PEI@CuNCs by DOX was attributed to internal filtration effect (IFE). This work showed great prospects for detecting DOX.
- Copper nanoclusters,
- Fluorescence,
- Polyethyleneimine,
- Antibiotic,
- Doxorubicin hydrochloride
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