Citation: LIU Lin, BAN Yu, WEI Yue, WANG Ming-Zhe, CHE Chao-Yue, SHI Heng-Chong, ZHANG Xu, LUAN Shi-Fang. Preparation and Analysis of Antibacterial and Hemostatic Properties of Multifunctional Sodium Alginate Sponge[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 1986-1994. doi: 10.19756/j.issn.0253-3820.210677 shu

Preparation and Analysis of Antibacterial and Hemostatic Properties of Multifunctional Sodium Alginate Sponge

LIU Lin ^1,2 ,
BAN Yu ¹ ,
WEI Yue ³ ,
WANG Ming-Zhe ¹ ,
CHE Chao-Yue ¹ ,
SHI Heng-Chong ^1,2,, ,
ZHANG Xu ¹ ,
LUAN Shi-Fang ^1,2

1.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
University of Science and Technology of China, Hefei 230026, China;
3.
Shandong Weigao Orthopaedic Device Co., Ltd., Weihai 264200, China

Corresponding author: SHI Heng-Chong, shihc@ciac.ac.cn
Received Date: 12 August 2021
Revised Date: 10 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.51973221), the Major Science and Technology Innovation Project of Shandong Province, China (No.2019JZZY011105), the Biomedical Materials Production Demonstration Platform of the Ministry of Industry and Information Technology of the People's Republic of China (No.TC190H32V/1) and the High-Tech Research & Development Program of the CAS-WEGO Group.

A multifunctional hemostatic sponge (CIP-HNTs/SAs) with rapid hemostasis and high efficiency antibacterial property was built by freeze-drying based on ciprofloxacin (CIP), halloysite (HNTs) and sodium alginate (SA). Fourier transform infrared (FTIR) spectroscopy, scanning electron microscope (SEM), density method and weighing method were used to detect the composition, porous structure, porosity and water absorption of the sodium alginate sponge (HNTs/SAs). Blood clotting time (BCT) and blood clotting index (BCI) were applied to test the hemostatic of HNTs/SAs and the optimal halloysite loading ratio was selected by these tests. Furthermore, the activated partial thrombin time (APTT) and prothrombin time (PT) were used to explore the mechanism of sponge hemostasis. The halloysite hollow tube structure could load with antibacterial drugs. The components of halloysite loaded with antibacterial drugs were characterized by energy dispersive spectroscopy (EDS) and FTIR spectroscopy, and the loaded antibacterial drugs were quantitatively detected by ultraviolet (UV) absorption spectrum. The long-term antibacterial activity of CIP-HNTs/SAs was tested by the agar plate counting methodand inhibition zone, and the biocompatibility of CIP-HNTs/SAs was tested by the cell proliferation test kit (CCK-8) and the hemolysis rate of red blood cells. The results showed that the porous and hydrophilia of sodium alginate sponge could quickly absorb water in the blood to promote blood cell aggregation. Moreover, calcium ions (Ca²⁺) and halloysite could enhance the body's own coagulation cascade activity. Comparing with the coagulation time in vitro of the commercial gelatin hemostatic sponge, the sodium alginate sponge (HNTs/SA) containing halloysite could achieve rapid hemostasis in only 18 s. Meanwhile, CIP-HNTs/SAs had great antibacterial property and 7-day long-lasting antibacterial performance.
- Sodium alginate,
- Halloysite,
- Antibacterial,
- Rapid hemostasis,
- Multifunctional sponge
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