Citation: XIAO Zhi-Ming, WANG Shi, SUO De-Cheng, LI Yang, YAO Ting, LOU Ying-Xia, ZHAO Xin-Xue, ZHANG Feng, FAN Xia. Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

XIAO Zhi-Ming ¹ ,
WANG Shi ¹ ,
SUO De-Cheng ¹ ,
LI Yang ¹ ,
YAO Ting ² ,
LOU Ying-Xia ² ,
ZHAO Xin-Xue ¹ ,
ZHANG Feng ³ ,
FAN Xia ^1,,

1.
China National Feed Quality Control Center, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2.
Beijing Institute of Feed Control, Beijing 100107, China;
3.
Reeko Instrument Co. Ltd., Xiamen 361000, China

Corresponding author: FAN Xia, fanxia@caas.cn
Received Date: 4 August 2021
Revised Date: 7 March 2022

Fund Project: Supported by the National Key Research and Development Program of China(No. 2018YFC1602302).

An ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method has been developed for determination of 22 kinds of β-agonists residues in swine, bovine and sheep muscle and liver samples. The animal food samples were extracted with enzymatic probe sonication(EPS) using β-glucuronidase/sulfatase enzymes, followed by clean-up using automatic solid-phase extraction and determination by UPLC-MS/MS. The target analytes were quantified by internal standard methods. Under the optimized EPS conditions, one sample could be exhaustively extracted within 120 s(average recovery 81.2%-100.7%), as compared with 16 h needed for the conventional enzymatic digestion, which was more suitable for high-throughput screening. Furthermore, the clean-up was conducted using automatic solid-phase extraction, and 36 samples could be performed in 30 min simultaneously. The work efficiency was significantly improved, and the relative standard deviations(RSDs) were relatively low(0.3%-7.9%).As compared with the traditional enzymatic digestion and solid-phase extraction techniques, the newly developed method was quick and easy to be performed, which was more suitable for high-throughput detection in routine residual analysis.
- β-Agonists,
- Enzymatic probe sonication,
- Automatic solid-phase extraction,
- Animal tissues
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