超声探针辅助酶解结合全自动固相萃取快速测定动物组织中<i>β</i>-受体激动剂残留

引用本文: 肖志明, 王石, 索德成, 李阳, 姚婷, 娄迎霞, 赵新雪, 张峰, 樊霞. 超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留[J]. 分析化学, 2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu

Citation: XIAO Zhi-Ming, WANG Shi, SUO De-Cheng, LI Yang, YAO Ting, LOU Ying-Xia, ZHAO Xin-Xue, ZHANG Feng, FAN Xia. Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues[J]. Chinese Journal of Analytical Chemistry, 2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

肖志明 ^1, ,
王石 ^1, ,
索德成 ^1, ,
李阳 ^1, ,
姚婷 ^2, ,
娄迎霞 ^2, ,
赵新雪 ^1, ,
张峰 ^3, ,
樊霞 ^{1,
,}

1.
中国农业科学院农业质量标准与检测技术研究所, 国家饲料质量检验检测中心(北京), 北京 100081;
2.
北京市饲料监察所, 北京 100107;
3.
睿科仪器(厦门)有限公司, 厦门 361000

通讯作者: 樊霞,E-mail:fanxia@caas.cn

基金项目:
“十三五”国家重点研发计划项目（No.2018YFC1602302）资助。

摘要: 建立了猪、牛、羊肌肉和肝脏中22种β-受体激动剂残留的超高效液相色谱-串联质谱（UPLC-MS/MS）分析方法。样品采用β-葡萄糖醛酸酶/芳基硫酸酯酶在超声探针辅助下进行快速酶解提取，酶解后在全自动固相萃取仪上净化，UPLC-MS/MS测定，采用内标法进行定量分析。结果表明，超声探针辅助酶解在120 s内即达到了传统酶解16 h的提取效率，回收率为81.2%~100.7%。结合全自动固相萃取的批量、自动化处理，30 min内可同时处理36份样品，显著提升了工作效率，避免了因人员操作导致的结果偏差，相对标准偏差低至0.3%~7.9%。与传统酶解和固相萃取方法相比，本方法具有快速、简便和高通量等优点，适用于大批量动物组织样品的日常监测。

关键词:

English

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

1.
China National Feed Quality Control Center, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2.
Beijing Institute of Feed Control, Beijing 100107, China;
3.
Reeko Instrument Co. Ltd., Xiamen 361000, China

Corresponding author: FAN Xia, fanxia@caas.cn

Received Date: 04 August 2021
Revised Date: 07 March 2022
Fund Project:
Supported by the National Key Research and Development Program of China(No. 2018YFC1602302).

Abstract: An ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method has been developed for determination of 22 kinds of β-agonists residues in swine, bovine and sheep muscle and liver samples. The animal food samples were extracted with enzymatic probe sonication(EPS) using β-glucuronidase/sulfatase enzymes, followed by clean-up using automatic solid-phase extraction and determination by UPLC-MS/MS. The target analytes were quantified by internal standard methods. Under the optimized EPS conditions, one sample could be exhaustively extracted within 120 s(average recovery 81.2%-100.7%), as compared with 16 h needed for the conventional enzymatic digestion, which was more suitable for high-throughput screening. Furthermore, the clean-up was conducted using automatic solid-phase extraction, and 36 samples could be performed in 30 min simultaneously. The work efficiency was significantly improved, and the relative standard deviations(RSDs) were relatively low(0.3%-7.9%).As compared with the traditional enzymatic digestion and solid-phase extraction techniques, the newly developed method was quick and easy to be performed, which was more suitable for high-throughput detection in routine residual analysis.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

通讯作者: 樊霞,E-mail:fanxia@caas.cn

English

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

Corresponding author: FAN Xia, fanxia@caas.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

通讯作者: 樊霞,E-mail:fanxia@caas.cn

English

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

Corresponding author: FAN Xia, fanxia@caas.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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