超声探针辅助酶解结合全自动固相萃取快速测定动物组织中<i>β</i>-受体激动剂残留

引用本文: 肖志明, 王石, 索德成, 李阳, 姚婷, 娄迎霞, 赵新雪, 张峰, 樊霞. 超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留[J]. 分析化学, 2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu

Citation: XIAO Zhi-Ming, WANG Shi, SUO De-Cheng, LI Yang, YAO Ting, LOU Ying-Xia, ZHAO Xin-Xue, ZHANG Feng, FAN Xia. Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues[J]. Chinese Journal of Analytical Chemistry, 2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

肖志明 ^1, ,
王石 ^1, ,
索德成 ^1, ,
李阳 ^1, ,
姚婷 ^2, ,
娄迎霞 ^2, ,
赵新雪 ^1, ,
张峰 ^3, ,
樊霞 ^{1,
,}

1.
中国农业科学院农业质量标准与检测技术研究所, 国家饲料质量检验检测中心(北京), 北京 100081;
2.
北京市饲料监察所, 北京 100107;
3.
睿科仪器(厦门)有限公司, 厦门 361000

通讯作者: 樊霞,E-mail:fanxia@caas.cn

基金项目:
“十三五”国家重点研发计划项目（No.2018YFC1602302）资助。

摘要: 建立了猪、牛、羊肌肉和肝脏中22种β-受体激动剂残留的超高效液相色谱-串联质谱（UPLC-MS/MS）分析方法。样品采用β-葡萄糖醛酸酶/芳基硫酸酯酶在超声探针辅助下进行快速酶解提取，酶解后在全自动固相萃取仪上净化，UPLC-MS/MS测定，采用内标法进行定量分析。结果表明，超声探针辅助酶解在120 s内即达到了传统酶解16 h的提取效率，回收率为81.2%~100.7%。结合全自动固相萃取的批量、自动化处理，30 min内可同时处理36份样品，显著提升了工作效率，避免了因人员操作导致的结果偏差，相对标准偏差低至0.3%~7.9%。与传统酶解和固相萃取方法相比，本方法具有快速、简便和高通量等优点，适用于大批量动物组织样品的日常监测。

关键词:

English

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

1.
China National Feed Quality Control Center, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2.
Beijing Institute of Feed Control, Beijing 100107, China;
3.
Reeko Instrument Co. Ltd., Xiamen 361000, China

Corresponding author: FAN Xia, fanxia@caas.cn

Received Date: 04 August 2021
Revised Date: 07 March 2022
Fund Project:
Supported by the National Key Research and Development Program of China(No. 2018YFC1602302).

Abstract: An ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method has been developed for determination of 22 kinds of β-agonists residues in swine, bovine and sheep muscle and liver samples. The animal food samples were extracted with enzymatic probe sonication(EPS) using β-glucuronidase/sulfatase enzymes, followed by clean-up using automatic solid-phase extraction and determination by UPLC-MS/MS. The target analytes were quantified by internal standard methods. Under the optimized EPS conditions, one sample could be exhaustively extracted within 120 s(average recovery 81.2%-100.7%), as compared with 16 h needed for the conventional enzymatic digestion, which was more suitable for high-throughput screening. Furthermore, the clean-up was conducted using automatic solid-phase extraction, and 36 samples could be performed in 30 min simultaneously. The work efficiency was significantly improved, and the relative standard deviations(RSDs) were relatively low(0.3%-7.9%).As compared with the traditional enzymatic digestion and solid-phase extraction techniques, the newly developed method was quick and easy to be performed, which was more suitable for high-throughput detection in routine residual analysis.

Key words:

1. [1]
  SU L, HU H, TIAN Y, JIA C, WANG L, ZHANG H, WANG J, ZHANG D. Anal. Chem., 2021, 93(23):8362-8369.SU L, HU H, TIAN Y, JIA C, WANG L, ZHANG H, WANG J, ZHANG D. Anal. Chem., 2021, 93(23):8362-8369.
2. [2]
  LI G, ZHANG X, ZHENG F, LIU J, WU D. Food Chem., 2020, 332:127431.LI G, ZHANG X, ZHENG F, LIU J, WU D. Food Chem., 2020, 332:127431.
3. [3]
  ZHANG W, WANG P L, SU X O. TrAC-Trends Anal. Chem., 2016, 85:1-16.ZHANG W, WANG P L, SU X O. TrAC-Trends Anal. Chem., 2016, 85:1-16.
4. [4]
  CASEY C R, ANDERSEN W C, WILLIAMS N T, NICKEL T J, AYRES P R. J. Agric. Food Chem., 2021, 69(4):117-1186.CASEY C R, ANDERSEN W C, WILLIAMS N T, NICKEL T J, AYRES P R. J. Agric. Food Chem., 2021, 69(4):117-1186.
5. [5]
  PENG C, ZHANG S, WU C, FENG Y, ZHAO D, WANG X, BAI Z. Food Chem., 2021, 355:129662.PENG C, ZHANG S, WU C, FENG Y, ZHAO D, WANG X, BAI Z. Food Chem., 2021, 355:129662.
6. [6]
  STELLA R, BOVO D, MASTRORILLI E, MANUALI E, PEZZOLATO M, BOZZETTA E, LEGA F, ANGELETTI R, BIANCOTTO G. Food Chem., 2021, 353:129366.STELLA R, BOVO D, MASTRORILLI E, MANUALI E, PEZZOLATO M, BOZZETTA E, LEGA F, ANGELETTI R, BIANCOTTO G. Food Chem., 2021, 353:129366.
7. [7]
  ROSSINI E L, KULYK D S, ANSU-GYEABOURH E, SAHRAEIAN T, PEZZA H R, BADU-TAWIAH A K. J. Am.Soc. Mass Spectrom., 2020, 31(6):1212-1222.ROSSINI E L, KULYK D S, ANSU-GYEABOURH E, SAHRAEIAN T, PEZZA H R, BADU-TAWIAH A K. J. Am.Soc. Mass Spectrom., 2020, 31(6):1212-1222.
8. [8]
  YUAN Y, NIE H, YIN J, HAN Y, LV Y, YAN H. Food Chem., 2020, 313:126155.YUAN Y, NIE H, YIN J, HAN Y, LV Y, YAN H. Food Chem., 2020, 313:126155.
9. [9]
  LI Xiao-Min, GAO Yan, SU Xiao-Ou, LI Yang, WANG Rui-Guo, ZHANG Yu, FU Jian-Jie. Chin. J. Anal. Chem., 2014, 42(12):1779-1784.李晓敏, 高燕, 苏晓鸥, 李阳, 王瑞国, 张瑜, 傅建捷.分析化学, 2014, 42(12):1779-1784.
10. [10]
  SUO D, WANG P, LI Y, WANG R, FAN X, SU X. J. Chromatogr. A, 2020, 1612:460624.SUO D, WANG P, LI Y, WANG R, FAN X, SU X. J. Chromatogr. A, 2020, 1612:460624.
11. [11]
  SUO De-Cheng, WEI Shu-Lin, XIAO Zhi-Ming, WANG Pei-Long, WANG Rui-Guo, LI Yang. Sci. Agric. Sin., 2019, 52(24):4613-4623.索德成, 魏书林, 肖志明, 王培龙, 王瑞国, 李阳.中国农业科学, 2019, 52(24):4613-4623.
12. [12]
  WANG X J, ZHANG F, DING F, LI W Q, CHEN Q Y, CHU X G, XU C B. J. Chromatogr. A, 2013, 1278:82-88.WANG X J, ZHANG F, DING F, LI W Q, CHEN Q Y, CHU X G, XU C B. J. Chromatogr. A, 2013, 1278:82-88.
13. [13]
  HERPIN L, BICHON E, RAMBAUD L, MONTEAU F, BIZEC B L. J. Chromatogr. B, 2018, 1086:130-137.HERPIN L, BICHON E, RAMBAUD L, MONTEAU F, BIZEC B L. J. Chromatogr. B, 2018, 1086:130-137.
14. [14]
  LI Yang, SU Xiao-Ou, ZHANG Wei, FAN Xia, WANG Pei-Long, WANG Rui-Guo, WANG Xiao. Chin. J. Anal.Chem., 2014, 42(5):71-722.李阳, 苏晓鸥, 张维, 樊霞, 王培龙, 王瑞国, 王骁.分析化学, 2014, 42(5):717-722.
15. [15]
  ZHU B L, CAI L Z, JIANG Z, LI Q, GUO X J. Microchem. J., 2019, 150:104082.ZHU B L, CAI L Z, JIANG Z, LI Q, GUO X J. Microchem. J., 2019, 150:104082.
16. [16]
  CHEN Qing-Ping, HAN Feng, WANG Shou-Ying, YU Hui-Juan. Chin. J. Anal. Lab., 2019, 38(6):695-700.陈清平, 韩峰, 王守英, 于慧娟.分析实验室, 2019, 38(6):695-700.
17. [17]
  GUO P, WAN J, ZHAN C, ZHU C, JIANG W, KE Y, DING S, WANG D. J. Chromatogr. B, 2018, 1096:122-134.GUO P, WAN J, ZHAN C, ZHU C, JIANG W, KE Y, DING S, WANG D. J. Chromatogr. B, 2018, 1096:122-134.
18. [18]
  BHARGAVA N, MOR R S, KUMAR K, SHARANAGAT V S. Ultrason. Sonochem., 2021, 70:105293.BHARGAVA N, MOR R S, KUMAR K, SHARANAGAT V S. Ultrason. Sonochem., 2021, 70:105293.
19. [19]
  GIANNAKOUDAKIS D A, ŁOMOT D, COLMENARES J C. Green Chem., 2020, 22(15):4896-4905GIANNAKOUDAKIS D A, ŁOMOT D, COLMENARES J C. Green Chem., 2020, 22(15):4896-4905
20. [20]
  ALARCON-ROJO A D, CARRILLO-LOPEZ L M, REYES-VILLAGRANA R, HUERTA-JIMÉNEZ M, GARCIAGALICIA I A. Ultrason. Sonochem., 2019, 55:369-382.ALARCON-ROJO A D, CARRILLO-LOPEZ L M, REYES-VILLAGRANA R, HUERTA-JIMÉNEZ M, GARCIAGALICIA I A. Ultrason. Sonochem., 2019, 55:369-382.
21. [21]
  XIAO Z, WANG J, GUO J, SUO D, WANG S, TIAN J, GUO L, FAN X. Food Addit. Contam., Part A, 2021, 38(2):268-279.XIAO Z, WANG J, GUO J, SUO D, WANG S, TIAN J, GUO L, FAN X. Food Addit. Contam., Part A, 2021, 38(2):268-279.
22. [22]
  XIAO Z, LI S, GUO J, RAO Z, LIU C, JIA Z, SUO D, WANG S, LI Y, FAN X. J. Chromatogr. A, 2017, 1530:51-58.XIAO Z, LI S, GUO J, RAO Z, LIU C, JIA Z, SUO D, WANG S, LI Y, FAN X. J. Chromatogr. A, 2017, 1530:51-58.
23. [23]
  SUO D, WANG R, WANG P, FAN X, SU X. J. Chromatogr. A, 2017, 1526:23-30.SUO D, WANG R, WANG P, FAN X, SU X. J. Chromatogr. A, 2017, 1526:23-30.
24. [24]
  MAO Si-Hao, LIU Zhu, LIANG Jing-Jing, CHEN Wan-Qin, XU Xiao-Ying, ZHAO Chao-Qun, WANG Feng. J.Anal. Sci., 2020, 36(4):572-578.毛思浩, 刘柱, 梁晶晶, 陈万勤, 徐潇颖, 赵超群, 王峰.分析科学学报, 2020, 36(4):572-578.
25. [25]
  PEIXOTO P S, SILVA E M P, OSÓRIO M V, BARREIROS L, LIMA J L F C, SEGUNDO M A. Anal. Methods, 2018, 10(18):2180-2186.PEIXOTO P S, SILVA E M P, OSÓRIO M V, BARREIROS L, LIMA J L F C, SEGUNDO M A. Anal. Methods, 2018, 10(18):2180-2186.
26. [26]
  SN/T 1924-2011. Determination of Clenbuterol, Ractopamine, Salbutamol and Terbutalin Residues in Foodstuffs of Animal Origin for Import and Export-HPLC-MS/MS Method. National Standards of the People's Republic of China.进出口动物源食品中克伦特罗、莱克多巴胺、沙丁胺醇和特布他林残留量的测定.液相色谱-质谱/质谱法.中华人民共和国国家标准. SN/T 1924-2011.

扫一扫看文章

计量

PDF下载量: 5
文章访问数: 682
HTML全文浏览量: 120

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

通讯作者: 樊霞,E-mail:fanxia@caas.cn

English

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

Corresponding author: FAN Xia, fanxia@caas.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

通讯作者: 樊霞,E-mail:fanxia@caas.cn

English

Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues

Corresponding author: FAN Xia, fanxia@caas.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs