超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

肖志明 王石 索德成 李阳 姚婷 娄迎霞 赵新雪 张峰 樊霞

引用本文: 肖志明, 王石, 索德成, 李阳, 姚婷, 娄迎霞, 赵新雪, 张峰, 樊霞. 超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留[J]. 分析化学, 2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu
Citation:  XIAO Zhi-Ming,  WANG Shi,  SUO De-Cheng,  LI Yang,  YAO Ting,  LOU Ying-Xia,  ZHAO Xin-Xue,  ZHANG Feng,  FAN Xia. Enzymatic Probe Sonication Coupled with Automatic Solid-Phase Extraction for Quick Determination of β-Agonists Residues in Animal Tissues[J]. Chinese Journal of Analytical Chemistry, 2022, 50(6): 957-963. doi: 10.19756/j.issn.0253-3820.210662 shu

超声探针辅助酶解结合全自动固相萃取快速测定动物组织中β-受体激动剂残留

    通讯作者: 樊霞,E-mail:fanxia@caas.cn
  • 基金项目:

    “十三五”国家重点研发计划项目(No.2018YFC1602302)资助。

摘要: 建立了猪、牛、羊肌肉和肝脏中22种β-受体激动剂残留的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法。样品采用β-葡萄糖醛酸酶/芳基硫酸酯酶在超声探针辅助下进行快速酶解提取,酶解后在全自动固相萃取仪上净化,UPLC-MS/MS测定,采用内标法进行定量分析。结果表明,超声探针辅助酶解在120 s内即达到了传统酶解16 h的提取效率,回收率为81.2%~100.7%。结合全自动固相萃取的批量、自动化处理,30 min内可同时处理36份样品,显著提升了工作效率,避免了因人员操作导致的结果偏差,相对标准偏差低至0.3%~7.9%。与传统酶解和固相萃取方法相比,本方法具有快速、简便和高通量等优点,适用于大批量动物组织样品的日常监测。

English


    1. [1]

      SU L, HU H, TIAN Y, JIA C, WANG L, ZHANG H, WANG J, ZHANG D. Anal. Chem., 2021, 93(23):8362-8369.SU L, HU H, TIAN Y, JIA C, WANG L, ZHANG H, WANG J, ZHANG D. Anal. Chem., 2021, 93(23):8362-8369.

    2. [2]

      LI G, ZHANG X, ZHENG F, LIU J, WU D. Food Chem., 2020, 332:127431.LI G, ZHANG X, ZHENG F, LIU J, WU D. Food Chem., 2020, 332:127431.

    3. [3]

      ZHANG W, WANG P L, SU X O. TrAC-Trends Anal. Chem., 2016, 85:1-16.ZHANG W, WANG P L, SU X O. TrAC-Trends Anal. Chem., 2016, 85:1-16.

    4. [4]

      CASEY C R, ANDERSEN W C, WILLIAMS N T, NICKEL T J, AYRES P R. J. Agric. Food Chem., 2021, 69(4):117-1186.CASEY C R, ANDERSEN W C, WILLIAMS N T, NICKEL T J, AYRES P R. J. Agric. Food Chem., 2021, 69(4):117-1186.

    5. [5]

      PENG C, ZHANG S, WU C, FENG Y, ZHAO D, WANG X, BAI Z. Food Chem., 2021, 355:129662.PENG C, ZHANG S, WU C, FENG Y, ZHAO D, WANG X, BAI Z. Food Chem., 2021, 355:129662.

    6. [6]

      STELLA R, BOVO D, MASTRORILLI E, MANUALI E, PEZZOLATO M, BOZZETTA E, LEGA F, ANGELETTI R, BIANCOTTO G. Food Chem., 2021, 353:129366.STELLA R, BOVO D, MASTRORILLI E, MANUALI E, PEZZOLATO M, BOZZETTA E, LEGA F, ANGELETTI R, BIANCOTTO G. Food Chem., 2021, 353:129366.

    7. [7]

      ROSSINI E L, KULYK D S, ANSU-GYEABOURH E, SAHRAEIAN T, PEZZA H R, BADU-TAWIAH A K. J. Am.Soc. Mass Spectrom., 2020, 31(6):1212-1222.ROSSINI E L, KULYK D S, ANSU-GYEABOURH E, SAHRAEIAN T, PEZZA H R, BADU-TAWIAH A K. J. Am.Soc. Mass Spectrom., 2020, 31(6):1212-1222.

    8. [8]

      YUAN Y, NIE H, YIN J, HAN Y, LV Y, YAN H. Food Chem., 2020, 313:126155.YUAN Y, NIE H, YIN J, HAN Y, LV Y, YAN H. Food Chem., 2020, 313:126155.

    9. [9]

      LI Xiao-Min, GAO Yan, SU Xiao-Ou, LI Yang, WANG Rui-Guo, ZHANG Yu, FU Jian-Jie. Chin. J. Anal. Chem., 2014, 42(12):1779-1784.李晓敏, 高燕, 苏晓鸥, 李阳, 王瑞国, 张瑜, 傅建捷.分析化学, 2014, 42(12):1779-1784.

    10. [10]

      SUO D, WANG P, LI Y, WANG R, FAN X, SU X. J. Chromatogr. A, 2020, 1612:460624.SUO D, WANG P, LI Y, WANG R, FAN X, SU X. J. Chromatogr. A, 2020, 1612:460624.

    11. [11]

      SUO De-Cheng, WEI Shu-Lin, XIAO Zhi-Ming, WANG Pei-Long, WANG Rui-Guo, LI Yang. Sci. Agric. Sin., 2019, 52(24):4613-4623.索德成, 魏书林, 肖志明, 王培龙, 王瑞国, 李阳.中国农业科学, 2019, 52(24):4613-4623.

    12. [12]

      WANG X J, ZHANG F, DING F, LI W Q, CHEN Q Y, CHU X G, XU C B. J. Chromatogr. A, 2013, 1278:82-88.WANG X J, ZHANG F, DING F, LI W Q, CHEN Q Y, CHU X G, XU C B. J. Chromatogr. A, 2013, 1278:82-88.

    13. [13]

      HERPIN L, BICHON E, RAMBAUD L, MONTEAU F, BIZEC B L. J. Chromatogr. B, 2018, 1086:130-137.HERPIN L, BICHON E, RAMBAUD L, MONTEAU F, BIZEC B L. J. Chromatogr. B, 2018, 1086:130-137.

    14. [14]

      LI Yang, SU Xiao-Ou, ZHANG Wei, FAN Xia, WANG Pei-Long, WANG Rui-Guo, WANG Xiao. Chin. J. Anal.Chem., 2014, 42(5):71-722.李阳, 苏晓鸥, 张维, 樊霞, 王培龙, 王瑞国, 王骁.分析化学, 2014, 42(5):717-722.

    15. [15]

      ZHU B L, CAI L Z, JIANG Z, LI Q, GUO X J. Microchem. J., 2019, 150:104082.ZHU B L, CAI L Z, JIANG Z, LI Q, GUO X J. Microchem. J., 2019, 150:104082.

    16. [16]

      CHEN Qing-Ping, HAN Feng, WANG Shou-Ying, YU Hui-Juan. Chin. J. Anal. Lab., 2019, 38(6):695-700.陈清平, 韩峰, 王守英, 于慧娟.分析实验室, 2019, 38(6):695-700.

    17. [17]

      GUO P, WAN J, ZHAN C, ZHU C, JIANG W, KE Y, DING S, WANG D. J. Chromatogr. B, 2018, 1096:122-134.GUO P, WAN J, ZHAN C, ZHU C, JIANG W, KE Y, DING S, WANG D. J. Chromatogr. B, 2018, 1096:122-134.

    18. [18]

      BHARGAVA N, MOR R S, KUMAR K, SHARANAGAT V S. Ultrason. Sonochem., 2021, 70:105293.BHARGAVA N, MOR R S, KUMAR K, SHARANAGAT V S. Ultrason. Sonochem., 2021, 70:105293.

    19. [19]

      GIANNAKOUDAKIS D A, ŁOMOT D, COLMENARES J C. Green Chem., 2020, 22(15):4896-4905GIANNAKOUDAKIS D A, ŁOMOT D, COLMENARES J C. Green Chem., 2020, 22(15):4896-4905

    20. [20]

      ALARCON-ROJO A D, CARRILLO-LOPEZ L M, REYES-VILLAGRANA R, HUERTA-JIMÉNEZ M, GARCIAGALICIA I A. Ultrason. Sonochem., 2019, 55:369-382.ALARCON-ROJO A D, CARRILLO-LOPEZ L M, REYES-VILLAGRANA R, HUERTA-JIMÉNEZ M, GARCIAGALICIA I A. Ultrason. Sonochem., 2019, 55:369-382.

    21. [21]

      XIAO Z, WANG J, GUO J, SUO D, WANG S, TIAN J, GUO L, FAN X. Food Addit. Contam., Part A, 2021, 38(2):268-279.XIAO Z, WANG J, GUO J, SUO D, WANG S, TIAN J, GUO L, FAN X. Food Addit. Contam., Part A, 2021, 38(2):268-279.

    22. [22]

      XIAO Z, LI S, GUO J, RAO Z, LIU C, JIA Z, SUO D, WANG S, LI Y, FAN X. J. Chromatogr. A, 2017, 1530:51-58.XIAO Z, LI S, GUO J, RAO Z, LIU C, JIA Z, SUO D, WANG S, LI Y, FAN X. J. Chromatogr. A, 2017, 1530:51-58.

    23. [23]

      SUO D, WANG R, WANG P, FAN X, SU X. J. Chromatogr. A, 2017, 1526:23-30.SUO D, WANG R, WANG P, FAN X, SU X. J. Chromatogr. A, 2017, 1526:23-30.

    24. [24]

      MAO Si-Hao, LIU Zhu, LIANG Jing-Jing, CHEN Wan-Qin, XU Xiao-Ying, ZHAO Chao-Qun, WANG Feng. J.Anal. Sci., 2020, 36(4):572-578.毛思浩, 刘柱, 梁晶晶, 陈万勤, 徐潇颖, 赵超群, 王峰.分析科学学报, 2020, 36(4):572-578.

    25. [25]

      PEIXOTO P S, SILVA E M P, OSÓRIO M V, BARREIROS L, LIMA J L F C, SEGUNDO M A. Anal. Methods, 2018, 10(18):2180-2186.PEIXOTO P S, SILVA E M P, OSÓRIO M V, BARREIROS L, LIMA J L F C, SEGUNDO M A. Anal. Methods, 2018, 10(18):2180-2186.

    26. [26]

      SN/T 1924-2011. Determination of Clenbuterol, Ractopamine, Salbutamol and Terbutalin Residues in Foodstuffs of Animal Origin for Import and Export-HPLC-MS/MS Method. National Standards of the People's Republic of China.进出口动物源食品中克伦特罗、莱克多巴胺、沙丁胺醇和特布他林残留量的测定.液相色谱-质谱/质谱法.中华人民共和国国家标准. SN/T 1924-2011.

  • 加载中
计量
  • PDF下载量:  3
  • 文章访问数:  208
  • HTML全文浏览量:  26
文章相关
  • 收稿日期:  2021-08-04
  • 修回日期:  2022-03-07
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章