Citation: ZHAO Dan, MA Xiao, LI Na, WANG Fang, CHEN Chuan-Xia, SUN Jian. Research Progress of Fluorescent Detection Methods for Alkaline Phosphatase Activity[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1804-1815. doi: 10.19756/j.issn.0253-3820.210654 shu

Research Progress of Fluorescent Detection Methods for Alkaline Phosphatase Activity

ZHAO Dan ¹ ,
MA Xiao ^1,, ,
LI Na ¹ ,
WANG Fang ¹ ,
CHEN Chuan-Xia ^2,, ,
SUN Jian ^3,,

1.
Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang 471023, China;
2.
School of Materials Science and Engineering, University of Jinan, Jinan 250022, China;
3.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: MA Xiao, CHEN Chuan-Xia, SUN Jian,
Received Date: 28 July 2021
Revised Date: 12 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos.22104046, 21974132, 21904048), the Natural Science Foundation of Henan Province, China (No.202300410285), the Project of Science and Technology Department of Henan Province, China (Nos.212102210122, 212102310409), the Scientific Research Foundation of Luoyang Institute of Science and Technology (No.2017BZ16) and the Youth Innovation Promotion Association, Chinese Academy of Sciences (No.2018258).

Alkaline phosphatase (ALP), which can catalyze the dephosphorylation of various phosphorylated species, is an essential enzyme in a variety of mammalian tissues. The exploration of novel approaches for detection of ALP with high selectivity and sensitivity and intracellular imaging in living organisms is of vital importance in human health, clinical diagnosis and environmental analysis. With the development of fluorescent probes, many advantages for ALP detection, such as simple operation, non-invasive, in-situ detection, real-time imaging and so on, have been gradually highlighted. The fluorescent methods are categorized by different response mechanisms for ALP activity sensing:difference in the fluorescence between the phosphorylated substrate and corresponding product; the interaction of certain metal ions and phosphorylated substrates; the in-situ fluorogenic reaction drived by the ALP-enabled dephosphorylation process; inner filter effect; the fluorescence energy transfer system and so on. In this review, we focused on the research progresses of the fluorescent methods in detection of ALP in recent years. Furthermore, the prospects of ALP in the near future were also discussed.
- Alkaline phosphatase,
- Fluorescence detection,
- Response mechanism,
- Phosphorylated species,
- Review
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