Citation: TANG Jin-Lan, BAO Yi-Lin, WU Jin-Jin, SHANG Lin-Wei, SHANG Hui, XU Zhi-Bing, WANG Hui-Jie, YIN Jian-Hua. Study on Breast Tissue Cancerization by Polarized Micro-Raman Spectroscopy[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(12): 2048-2054. doi: 10.19756/j.issn.0253-3820.210602 shu

Study on Breast Tissue Cancerization by Polarized Micro-Raman Spectroscopy

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China

Corresponding author: WANG Hui-Jie, YIN Jian-Hua,
Received Date: 1 July 2021
Revised Date: 27 September 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.61378087), the Six Talent Peaks Project of Jiangsu Province, China (No.SWYY-034) and the Open Fund for 2020 Graduate Innovation Base (Laboratory) of Nanjing University of Aeronautics and Astronautics (No.kfjj20200315).

The polarized micro-Raman spectra (PMRS) technique can provide information about the orientations and ordering of molecules in samples without any special sample preparation or staining. In this work, PMRS was used to collect Raman spectra of normal and cancerous breast tissues. By spectral analysis and difference analysis, it was found that PMRS could significantly amplify the differences of main characteristic bands other than amide Ⅰ band, even the bands of proline (921 cm^-1) and phenylalanine (1032 cm^-1) which had unobvious difference in conventional micro-Raman spectroscopy. The results indicated that PMRS was much efficient to identify breast cancer tissue. The bimodal intensity ratio of the amide Ⅲ band (I₁₂₄₇/I₁₂₆₉) was lower than 1 in cancerous tissues, no matter excited with parallel or perpendicular polarized light, but it was lower and greater than 1 in normal tissue when excited with parallel and perpendicular polarized light, respectively, indicating that collagen fibers lost their original orientation in cancerous tissues. Meanwhile, it was proved that this phenomenon was related to the increase in the content of hydroxylated amino acids of collagen fibers after canceration. The information obtained in this study helped to elucidate the molecular mechanism of induced intravasation during breast cancer invasion more clearly. It suggested that PMRS had more potential to be developed as an effective tool for clinical diagnosis of breast cancer, to assist in the clinical diagnosis and treatment of invasive breast cancer.
- Polarized micro-Raman spectroscopy,
- Breast cancer,
- Collagen,
- Difference enhancement
1. [1]
  FERLAY J, COLOMBET M, SOERJOMATARAM I, PARKIN DONALD M, PIÑEROS M, ZNAOR A, BRAY F. Int. J. Cancer, 2021, 149(4):778-789.
2. [2]
  LECHAGO J. Arch. Pathol. Lab. Med., 2005, 129(12):1529-1531.
3. [3]
4. [4]
5. [5]
  BARROSO E M, SCHUT T B, CASPERS P J, SANTOS I P, WOLVIUS E B, KOLJENOVIĆS, PUPPELS G J.J. Raman Spectrosc., 2018, 49(4):699-709.
6. [6]
7. [7]
  TANAKA M, YOUNG R J. J. Mater. Sci., 2006, 41(3):963-991.
8. [8]
  LY E, PIOT O, DURLACH A, BERNARD P, MANFAIT M. Appl. Spectrosc., 2008, 62(10):1088-1094.
9. [9]
  DANIEL A, PRAKASARAO A, DORNADULA K, GANESAN S. Spectrochim. Acta, Part A, 2016, 152:58-63.
10. [10]
  ZHAO J, LUI H, MCLEAN D I, ZENG H. Appl. Spectros., 2007, 61(11):1225-1232.
11. [11]
  SHANG L W, MA D Y, FU J J, LU Y F, YIN J H. Biomed. Opt. Express, 2020, 11(7):3673-3683.
12. [12]
  LAZARO-PACHECO D, SHAABAN A M, REHMAN S, REHMAN I. Appl. Spectrosc. Rev., 2019, 55(1):1-37.
13. [13]
  REHMAN S, MOVASAGHI Z, TUCKER A T, JOEL S P, REHMAN I U. J. Raman Spectrosc., 2010, 38(10):1345-1351.
14. [14]
  KNEIPP J, SCHUT T B, KLIFFEN M, MENKE-PLUIJMERS M, PUPPELS G. Vib. Spectrosc., 2003, 32(1):67-74.
15. [15]
  TALARI A C S, MOVASAGHI Z, REHMAN S, REHMAN I. Appl. Spectrosc. Rev., 2015, 50(1):46-111.
16. [16]
  MEUTTER J D, GOORMAGHTIGH E. Eur. Biophys. J., 2021, 50:641-651.
17. [17]
  BONIFACIO A, SERGO V. Vib.Spectrosc., 2010, 53(2):314-317.
18. [18]
  HAN W, CHEN S, WEI Y, FAN Q, LIU L. Proc. Natl. Acad. Sci. U. S. A., 2016, 113(40):11208-11213.
19. [19]
  HOLMES D F, LU Y, STARBORG T, KADLER K E. Curr. Top. Dev. Bio., 2018, 130:107-142.
20. [20]
  RITCHIE R O, BUEHLER M J, HANSMA P. Phys.Today, 2009, 62(6):41-47.
21. [21]
  SALO A M, MYLLYHARJU J. Exp. Dermatol., 2021, 30(1):38-49.
22. [22]
  RUNEL G, LOPEZ-RAMIREZ N, CHLASTA J, MASSE I. Cells, 2021, 10(4):887-900.
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