肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析

郭佳 魏丽萍 陈志营 何成彦

引用本文: 郭佳, 魏丽萍, 陈志营, 何成彦. 肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析[J]. 分析化学, 2021, 49(9): 1563-1571. doi: 10.19756/j.issn.0253-3820.210590 shu
Citation:  GUO Jia,  WEI Li-Ping,  CHEN Zhi-Ying,  HE Cheng-Yan. Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1563-1571. doi: 10.19756/j.issn.0253-3820.210590 shu

肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析

    通讯作者: 陈志营,E-mail:zychen@jlu.edu.cn; 何成彦,E-mail:chengyanhe469@vip.sina.com
  • 基金项目:

    吉林省科技厅项目(Nos.20190201215JC,20200404193YY)资助。

摘要: 肾透明细胞癌中介导蛋白质棕榈酰化修饰的蛋白酰基转移酶表达异常,提示肾透明细胞癌可能存在蛋白质棕榈酰化修饰异常改变,并与肿瘤代谢紊乱或致癌信号通路的激活密切相关。本研究采用优化的酰基-生物素置换法(Acyl-biotinyl exchange,ABE),结合蛋白质组学技术,对肾透明细胞癌组织中S-棕榈酰化修饰蛋白进行全面分析,共鉴定出92种高可信度的棕榈酰化修饰蛋白,包括细胞骨架相关蛋白4、磷酸丙糖异构酶、脂肪分化相关蛋白、膜联蛋白A4、微管相关蛋白tau蛋白和钙联蛋白等。通过蛋白质印迹分析技术对其中的脂肪分化相关蛋白(Adipose differentiation-related protein,ADRP)棕榈酰化修饰进行了验证。本研究提供了一种改进的适合分析组织样本的ABE实验方法,所得肾透明细胞癌组织中的S-棕榈酰化修饰蛋白的分析结果可为肾透明细胞癌早期诊断、治疗和预后的分子靶点等方面的研究提供基础数据,对进一步深入研究肾透明细胞癌发病机制具有重要的参考价值。

English


    1. [1]

      MANCINI M, RIGHETTO M, BAGGIO G. Int. J. Mol. Sci., 2020, 21(9):3378.MANCINI M, RIGHETTO M, BAGGIO G. Int. J. Mol. Sci., 2020, 21(9):3378.

    2. [2]

      LINEHAN W M, RICKETTS C J. Nat. Rev. Urol., 2019, 16(9):539-552.LINEHAN W M, RICKETTS C J. Nat. Rev. Urol., 2019, 16(9):539-552.

    3. [3]

      HAMMERS H. Curr. Opin. Urol., 2016, 26(6):543-547.HAMMERS H. Curr. Opin. Urol., 2016, 26(6):543-547.

    4. [4]

      KOTECHA R R, MOTZER R J, VOSS M H. Nat. Rev. Clin. Oncol., 2019, 16(10):621-633.KOTECHA R R, MOTZER R J, VOSS M H. Nat. Rev. Clin. Oncol., 2019, 16(10):621-633.

    5. [5]

      JOHANN D J, WEI B R, PRIETO D A, CHAN K C, YE X, VALERA V A, SIMPSON R M, RUDNICK P A, XIAO Z, ISSAQ H J, LINEHAN W M, STEIN S E, VEENSTRA T D, BLONDER J. Anal. Chem., 2010, 82(5):1584-1588.JOHANN D J, WEI B R, PRIETO D A, CHAN K C, YE X, VALERA V A, SIMPSON R M, RUDNICK P A, XIAO Z, ISSAQ H J, LINEHAN W M, STEIN S E, VEENSTRA T D, BLONDER J. Anal. Chem., 2010, 82(5):1584-1588.

    6. [6]

      CHEN C J, CHOU C Y, SHU K H, CHEN H C, WANG M C, CHANG C C, HSU B G, WU M S, YANG Y L, LIAO W L, YANG C, HSIAO Y T, HUANG C C. J. Proteome Res., 2021, 20(5):2953-2963.CHEN C J, CHOU C Y, SHU K H, CHEN H C, WANG M C, CHANG C C, HSU B G, WU M S, YANG Y L, LIAO W L, YANG C, HSIAO Y T, HUANG C C. J. Proteome Res., 2021, 20(5):2953-2963.

    7. [7]

      PERROUD B, ISHIMARU T, BOROWSKY A D, WEISS R H. Mol. Cell. Proteomics, 2009, 8(5):971-985.PERROUD B, ISHIMARU T, BOROWSKY A D, WEISS R H. Mol. Cell. Proteomics, 2009, 8(5):971-985.

    8. [8]

      HWA J S, KIM H J, GOO B M, PARK H J, KIM C W, CHUNG K H, PARK H C, CHANG S H, KIM Y W, KIM D R, CHO G J, CHOI W S, KANG K R. Proteomics, 2006, 6(3):1077-1084.HWA J S, KIM H J, GOO B M, PARK H J, KIM C W, CHUNG K H, PARK H C, CHANG S H, KIM Y W, KIM D R, CHO G J, CHOI W S, KANG K R. Proteomics, 2006, 6(3):1077-1084.

    9. [9]

      NUERRULA Y, REXIATI M, LIU Q, WANG Y J. Cancer Biomarkers, 2015, 15(4):485-491.NUERRULA Y, REXIATI M, LIU Q, WANG Y J. Cancer Biomarkers, 2015, 15(4):485-491.

    10. [10]

      LI H, KOSREL S A, DIMARTINO S E, HASHEMI GHEINANI A, FROEHLICH J W, LEE R S. J. Proteome Res., 2021, 20(5):2662-2672.LI H, KOSREL S A, DIMARTINO S E, HASHEMI GHEINANI A, FROEHLICH J W, LEE R S. J. Proteome Res., 2021, 20(5):2662-2672.

    11. [11]

      AGGELIS V, CRAVEN R A, PENG J, HARDEN P, CAIRNS D A, MAHER E R, TONGE R, SELBY P J, BANKS R E. Proteomics, 2009, 9(8):2118-2130.AGGELIS V, CRAVEN R A, PENG J, HARDEN P, CAIRNS D A, MAHER E R, TONGE R, SELBY P J, BANKS R E. Proteomics, 2009, 9(8):2118-2130.

    12. [12]

      PINTO J, AMARO F, LIMA A R, CARVALHO-MAIA C, JERÓNIMO C, HENRIQUE R, BASTOS M L, CARVALHO M, GUEDES DE PINHO P. Proteome Res., 2021, 20(6):3068-3077.PINTO J, AMARO F, LIMA A R, CARVALHO-MAIA C, JERÓNIMO C, HENRIQUE R, BASTOS M L, CARVALHO M, GUEDES DE PINHO P. Proteome Res., 2021, 20(6):3068-3077.

    13. [13]

      LIN L, ZHENG J, YU Q, CHEN W, XING J, CHEN C, TIAN R. J. Proteomics, 2018, 174:9-16.LIN L, ZHENG J, YU Q, CHEN W, XING J, CHEN C, TIAN R. J. Proteomics, 2018, 174:9-16.

    14. [14]

      ATRIH A, MUDALIAR M A, ZAKIKHANI P, LAMONT D J, HUANG JT, BRAY S E, BARTON G, FLEMING S, NABI G. Br. J. Cancer, 2014, 110(6):1622-1633.ATRIH A, MUDALIAR M A, ZAKIKHANI P, LAMONT D J, HUANG JT, BRAY S E, BARTON G, FLEMING S, NABI G. Br. J. Cancer, 2014, 110(6):1622-1633.

    15. [15]

      GUO Jia, LIU Jin-Hua, YANG Zhao-Wei, LI Yi, HE Cheng-Yan. Chin. J. Anal. Chem., 2020, 48(10):1351-1358. 郭佳, 刘金华, 杨照微, 李毅, 何成彦. 分析化学, 2020, 48(10):1351-1358.

    16. [16]

      SUN N, WANG Y, WANG J, SUN W, YANG J, LIU N. Anal. Chem., 2020, 92(12):8292-8297.SUN N, WANG Y, WANG J, SUN W, YANG J, LIU N. Anal. Chem., 2020, 92(12):8292-8297.

    17. [17]

      YAN Min, LIU Jing, XIA Tian, XU Guo-Wang, PIAO Hai-Long. Chin. J. Anal. Chem., 2020, 37(8):887-896. 颜敏, 刘静, 夏天, 许国旺, 朴海龙. 色谱, 2019, 37(8):887-896.

    18. [18]

      ZHANG J, PENG Q, ZHAO W, SUN W, YANG J, LIU N. J. Proteome Res., 2021, 20(1):110-121.ZHANG J, PENG Q, ZHAO W, SUN W, YANG J, LIU N. J. Proteome Res., 2021, 20(1):110-121.

    19. [19]

      CHEN J J, FAN Y, BOEHNING D. Front. Mol. Biosci., 2021, 8:656440.CHEN J J, FAN Y, BOEHNING D. Front. Mol. Biosci., 2021, 8:656440.

    20. [20]

      WANG Y, YANG W. Proteome Res., 2021, 20(1):14-26.WANG Y, YANG W. Proteome Res., 2021, 20(1):14-26.

    21. [21]

      LIU Z, LIU C, XIAO M, HAN Y, ZHANG S, XU B. Front. Oncol., 2020, 10:565414.LIU Z, LIU C, XIAO M, HAN Y, ZHANG S, XU B. Front. Oncol., 2020, 10:565414.

    22. [22]

      WANG Zi-Jian, GAO Xiang, YANG Jing-Bo, SUN Wang-Chun, WU Dong-Lin, PENG Qi-Sheng, LIU Ning. Chin. J. Anal. Chem., 2016, 44(10):1521-1526. 王子健, 高祥, 杨静波, 孙万春, 吴东林, 彭其胜, 刘宁. 分析化学, 2016, 44(10):1521-1526.

    23. [23]

      SHI Q H, GAO S, SONG LN, ZHAO Y M, LI X O, WU J L, HE C Y, LI H J, ZHAO H F. Proteome Sci., 2015, 13(1):1.SHI Q H, GAO S, SONG LN, ZHAO Y M, LI X O, WU J L, HE C Y, LI H J, ZHAO H F. Proteome Sci., 2015, 13(1):1.

    24. [24]

      SUN N, SUN W, LI S, YANG J, YANG L, QUAN G, GAO X, WANG Z, CHENG X, LI Z, PENG Q, LIU N. Int. J. Mol. Sci., 2015, 16(11):25982-25998.SUN N, SUN W, LI S, YANG J, YANG L, QUAN G, GAO X, WANG Z, CHENG X, LI Z, PENG Q, LIU N. Int. J. Mol. Sci., 2015, 16(11):25982-25998.

    25. [25]

      DOWAL L, YANG W, FREEMAN M R, STEEN H, FLAUMENHAFT R. Blood, 2011, 118(13):e62-73.DOWAL L, YANG W, FREEMAN M R, STEEN H, FLAUMENHAFT R. Blood, 2011, 118(13):e62-73.

    26. [26]

      HU R, GUPTA R, WANG Z, WANG C, SUN H, SINGAMANENI S, KHARASCH E D, MORRISSEY J J. Kidney Int., 2019, 96(6):1417-1421.HU R, GUPTA R, WANG Z, WANG C, SUN H, SINGAMANENI S, KHARASCH E D, MORRISSEY J J. Kidney Int., 2019, 96(6):1417-1421.

    27. [27]

      SONG Y, ZHONG L, ZHOU J, LU M, XING T, MA L, SHEN J. Proteomics Clin. Appl., 2017, 11(11-12):1700066.SONG Y, ZHONG L, ZHOU J, LU M, XING T, MA L, SHEN J. Proteomics Clin. Appl., 2017, 11(11-12):1700066.

    28. [28]

      CAO Q, RUAN H, WANG K, SONG Z, BAO L, XU T, XIAO H, WANG C, CHENG G, TONG J, MENG X, LIU D, YANG H, CHEN K, ZHANG X. Int. J. Oncol., 2018, 53(1):137-147.CAO Q, RUAN H, WANG K, SONG Z, BAO L, XU T, XIAO H, WANG C, CHENG G, TONG J, MENG X, LIU D, YANG H, CHEN K, ZHANG X. Int. J. Oncol., 2018, 53(1):137-147.

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  • 收稿日期:  2021-06-26
  • 修回日期:  2021-08-05
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