Citation: LIU Hui, LIU Meng-Meng, YANG Yuan-Jie, LEI Yun, LIU Ai-Lin. Study on Electroosmotic Flow of 3D Print Based Microfluidic Electrophoresis Chip[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(11): 1937-1944. doi: 10.19756/j.issn.0253-3820.210526 shu

Study on Electroosmotic Flow of 3D Print Based Microfluidic Electrophoresis Chip

1.
Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350122, China;
2.
Department of Pharmacy, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China

Corresponding author: LIU Ai-Lin, ailinliu@fjmu.edu.cn
Received Date: 26 May 2021
Revised Date: 10 August 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.82072379), the Guiding Project of Science and Technology Plan of Fujian Province, China (No.2019Y0012) and Mid-Aged and Young Teachers Education Research Project of Fujian Province, China (No.JAT200147).

Two different types of 3D printers were employed for exploring the electroosmotic performance of microfluidic electrophoresis chip:one was Stereo lithography appearance (SLA) type using resin as material for design and preparation of four different kinds of micro-channels, i.e. regular triangle, square, 3/4 regular icosagon and 3/4 circle; the other was Fused deposition modeling (FDM) type using polymer filament as material for design and preparation of square channel. The influence of pH value of buffer solution, microchannel cross-section shape and types of 3D printers & craftsmanship of fabrication were investigated by current monitoring method. The results showed that the electroosmotic mobility of microchannel was negatively correlated with the pH value of buffer solution in the SLA 3D printed electrophoresis chip, whose surface carried positive charge. Among the four different microchannels, the square one had the largest μ_EOF value. As for the FDM 3D printed electrophoresis chip, it had opposite linear relation between EOF mobility and pH value, demonstrating its surface carried negative charge, but with better linearity and lower electroosmotic mobility. SLA type had multiple production potential but high viscosity resin, which was difficult to remove from microchannels, made the process complex and resulted in the final chip lack of fabrication stability. FDM type was advantageous in fabrication stability, but the diversity of microchannels' shape was limited. This study developed a new method for the fabrication of microchannels and explored the electroosmotic performance of them, which was expected to provide scientific theories for 3D printed microfluidic electrophoresis chip as well as its application in electrophoretic ananlysis.
- Microfluidic electrophoresis chip,
- 3D printing,
- Microchannel,
- Electroosmotic flow
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沈阳化工大学材料科学与工程学院沈阳 110142