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电喷雾离子化过程中蛋白质超多电荷离子生成方法研究进展

高晓梅 ,  尹欣驰 ,  谭思源 ,  戴新华 ,  龚晓云 ,  弓爱君

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引用本文: 高晓梅, 尹欣驰, 谭思源, 戴新华, 龚晓云, 弓爱君. 电喷雾离子化过程中蛋白质超多电荷离子生成方法研究进展[J]. 分析化学, 2021, 49(10): 1607-1618. doi: 10.19756/j.issn.0253-3820.210485 shu
Citation:  GAO Xiao-Mei,  YIN Xin-Chi,  TAN Si-Yuan,  DAI Xin-Hua,  GONG Xiao-Yun,  GONG Ai-Jun. Recent Advances in Supercharging of Proteins During Electrospray Ionization[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1607-1618. doi: 10.19756/j.issn.0253-3820.210485 shu

电喷雾离子化过程中蛋白质超多电荷离子生成方法研究进展

  • 1.

    北京科技大学化学与生物工程学院, 北京 100083;

  • 2.

    中国计量科学研究院前沿计量科学中心, 北京 100029

    • 通讯作者: 龚晓云,E-mail:gxy@nim.ac.cn; 弓爱君,E-mail:gongaijun@ustb.edu.cn
  • 基金项目:

    国家自然科学基金项目（No.21927812）和中国计量科学研究院基本科研业务费项目（No.AKY1932）资助。

摘要: 电喷雾离子化（Electrospray ionization，ESI）技术是目前常用的生物分子质谱离子化技术之一。在ESI过程中，蛋白质等生物大分子会携带多重电荷，形成多电荷离子。多电荷离子的形成，可有效降低待测离子的质荷比（Mass-to-charge ratio，m/z），扩大可检测分子量范围，提高检测灵敏度，为生物大分子的质谱分析带来较多便利。近年来，研究者提出了一些可在ESI过程中进一步提高蛋白质分子所带电荷数的方法，并将该方法称为蛋白质超多电荷化（Supercharging of proteins）方法。本文对蛋白质超多电荷化方法进行了系统的归纳和总结，综述了这些方法的离子化机理及影响因素，并对其应用进行了介绍。

English


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