新型比率型电化学生物传感器的构建及对BCR/ABL融合基因的检测

陈美君 刘雅兰 王颖 戈芷琪 张修华 王升富 何汉平

引用本文: 陈美君, 刘雅兰, 王颖, 戈芷琪, 张修华, 王升富, 何汉平. 新型比率型电化学生物传感器的构建及对BCR/ABL融合基因的检测[J]. 分析化学, 2022, 50(5): 692-700. doi: 10.19756/j.issn.0253-3820.210477 shu
Citation:  CHEN Mei-Jun,  LIU Ya-Lan,  WANG Ying,  GE Zhi-Qi,  ZHANG Xiu-Hua,  WANG Sheng-Fu,  HE Han-Ping. A Novel Ratiometric Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene[J]. Chinese Journal of Analytical Chemistry, 2022, 50(5): 692-700. doi: 10.19756/j.issn.0253-3820.210477 shu

新型比率型电化学生物传感器的构建及对BCR/ABL融合基因的检测

    通讯作者: 何汉平,E-mail:hehanping@hubu.edu.cn
  • 基金项目:

    省部共建生物催化与酶工程国家重点实验室开放基金项目(No.SKLBEE2020016)和国家自然科学基金项目(Nos.52171177,21575035)资助。

摘要: 采用普鲁士蓝(PB)和硫堇(Thi)两种电化学信号物质,同时引入核酸外切酶Ⅲ(Exo Ⅲ)辅助目标循环和杂交链式反应(HCR)两种信号放大策略,构建了一种超灵敏比率型电化学生物传感器,用于BCR/ABL融合基因的检测。首先,通过恒电位沉积法在玻碳电极表面形成一层普鲁士蓝(PB)薄膜,在其上固定捕获DNA。然后,将发夹DNA (H1)与目标DNA杂交配对,在Exo Ⅲ作用下,目标DNA被释放,可进入下一次循环,同时释放出发夹DNA的部分单链片段(Trigger-DNA),此单链可被PB膜和辅助DNA修饰电极捕获,继而在此修饰电极表面进行HCR反应,形成典型的Y形线性DNA串联体。此时,PB的电化学信号降低;另一方面,形成的串联体与Thi通过静电作用结合后,呈现高的Thi电化学响应。Thi和PB的电流响应信号比值(IT/IP)的对数与目标DNA的浓度对数在1 pmol/L~100 nmol/L范围内具有良好的线性关系,线性方程为lg (IT/IP)=1.094×lgC+0.156,检出限为0.19 pmol/L。本方法对BCR/ABL融合基因具有较高的选择性。此比率型电化学生物传感器为慢性粒细胞白血病(CML)的诊断和预后提供了一种简单可行的策略。

English


    1. [1]

      FIALKOW P J, GARTLER S M, YOSHIDA A. Proc. Natl. Acad. Sci. U.S.A., 1967, 58(4):1468-1471.FIALKOW P J, GARTLER S M, YOSHIDA A. Proc. Natl. Acad. Sci. U.S.A., 1967, 58(4):1468-1471.

    2. [2]

      KANTARJIAN H M, DEISSEROTH A, KURZROCK R, ESTROV Z, TALPAZ M. Blood, 1993, 82(3):691-703.KANTARJIAN H M, DEISSEROTH A, KURZROCK R, ESTROV Z, TALPAZ M. Blood, 1993, 82(3):691-703.

    3. [3]

      DALEY G Q, VANETTEN R A, BALTIMORE D. Science, 1990, 247(4944):824-830.DALEY G Q, VANETTEN R A, BALTIMORE D. Science, 1990, 247(4944):824-830.

    4. [4]

      LIN X H, WU P, CHEN W, ZHANG Y F, XIA X H. Talanta, 2007, 72(2):468-471.LIN X H, WU P, CHEN W, ZHANG Y F, XIA X H. Talanta, 2007, 72(2):468-471.

    5. [5]

      KANTARJIAN H M, KEATING M J, TALPAZ M, WALTERS R S, SIMTH T L, CORK A, MCCREDIE K B, FREIREICH E J. Am. J. Med., 1987, 83(3):445-454.KANTARJIAN H M, KEATING M J, TALPAZ M, WALTERS R S, SIMTH T L, CORK A, MCCREDIE K B, FREIREICH E J. Am. J. Med., 1987, 83(3):445-454.

    6. [6]

      HAMBLIN T J. Leuk. Res., 2002, 26(6):607-609.HAMBLIN T J. Leuk. Res., 2002, 26(6):607-609.

    7. [7]

      LANDSTROM A P, TEFFERI A. Leuk. Lymphoma, 2006, 47(3):397-402.LANDSTROM A P, TEFFERI A. Leuk. Lymphoma, 2006, 47(3):397-402.

    8. [8]

      MVLLER C, HENNIG E, FRANKE C, KRAHL R, LEIBLEIN S, NIEDERWIESER D, DEININGER M W. Cancer Genet. Cytogenet., 2002, 136(2):149-150.MVLLER C, HENNIG E, FRANKE C, KRAHL R, LEIBLEIN S, NIEDERWIESER D, DEININGER M W. Cancer Genet. Cytogenet., 2002, 136(2):149-150.

    9. [9]

      CORRENTE F, BELLESI S, METAFUNI E, PUGGIONI P L, MARIETTI S, CIMINELLO A M, ZA T, SORA F, FIANCHI L, SICA S, STEFANO V D, CHIUSOLO P. Cytometry, Part B, 2018, 94(3):468-476.CORRENTE F, BELLESI S, METAFUNI E, PUGGIONI P L, MARIETTI S, CIMINELLO A M, ZA T, SORA F, FIANCHI L, SICA S, STEFANO V D, CHIUSOLO P. Cytometry, Part B, 2018, 94(3):468-476.

    10. [10]

      VROTSOS E, GORGAN M, DIGIUSEPPE J A. Cytometry, Part B, 2018, 92(4):275-278.VROTSOS E, GORGAN M, DIGIUSEPPE J A. Cytometry, Part B, 2018, 92(4):275-278.

    11. [11]

      WU J L, HUANG Y, BIAN X, LI D, CHENG Q, DING S. Opt. Commun., 2016, 377:24-32.WU J L, HUANG Y, BIAN X, LI D, CHENG Q, DING S. Opt. Commun., 2016, 377:24-32.

    12. [12]

      XU Y, BIAN X, SANG Y, LI Y, LI D, CHENG W, YIN Y, JU H, DING S. Sci. Rep., 2016, 6:32370.XU Y, BIAN X, SANG Y, LI Y, LI D, CHENG W, YIN Y, JU H, DING S. Sci. Rep., 2016, 6:32370.

    13. [13]

      GULATI P, KAUR P, RAJAM M V, SRIVASTAVA T, ALI M A, MISHRA P, ISLAM S S. Sens. Actuators, B, 2018, 270:45-55.GULATI P, KAUR P, RAJAM M V, SRIVASTAVA T, ALI M A, MISHRA P, ISLAM S S. Sens. Actuators, B, 2018, 270:45-55.

    14. [14]

      GHOLIVAND M B, AKBARI A. Biosens. Bioelectron., 2019, 129:182-188.GHOLIVAND M B, AKBARI A. Biosens. Bioelectron., 2019, 129:182-188.

    15. [15]

      CIFTCI S, CANOVAS R, NEUMANN F, PAULRAJ T, NILSSON M, CRESPO G A, MADABOOSI N. Biosens. Bioelectron., 2020, 151:112002.CIFTCI S, CANOVAS R, NEUMANN F, PAULRAJ T, NILSSON M, CRESPO G A, MADABOOSI N. Biosens. Bioelectron., 2020, 151:112002.

    16. [16]

      WANG Cun, LIU Fan-Xin, HUI Jun-Min, ZOU Xiao-Chuan, DENG Ming-Chuan, ZHENG Tian-Ping, LIU Lin. Chin. J. Anal. Chem., 2020, 48(9):1185-1192. 王存, 刘蕃鑫, 惠俊敏, 邹晓川, 邓明川, 郑天平, 刘林. 分析化学, 2020, 48(9):1185-1192.

    17. [17]

      GE L, WANG W, LI F. Anal. Chem., 2017, 89(21):11560-11567.GE L, WANG W, LI F. Anal. Chem., 2017, 89(21):11560-11567.

    18. [18]

      GAI P P, GU C C, LI H Y, SUN X Z, LI F. Anal. Chem., 2017, 89(22):12293-12298.GAI P P, GU C C, LI H Y, SUN X Z, LI F. Anal. Chem., 2017, 89(22):12293-12298.

    19. [19]

      ZHU L P, ZHANG M Q, YE J, YAN M X, ZHU Q J, HUANG J S, YANG X R. Anal. Chem., 2020, 92(12):8614-8622.ZHU L P, ZHANG M Q, YE J, YAN M X, ZHU Q J, HUANG J S, YANG X R. Anal. Chem., 2020, 92(12):8614-8622.

    20. [20]

      ZHAO L, SUN R J, HE P, ZHANG X R. Anal. Chem., 2019, 91(22):14773-14779.ZHAO L, SUN R J, HE P, ZHANG X R. Anal. Chem., 2019, 91(22):14773-14779.

    21. [21]

      ZHANG W, LIU C, HAN K, WEI X O, XU Y W, ZHOU X B, ZHANG H, CHEN Z Y. Biosens. Bioelectron., 2020, 154:112091.ZHANG W, LIU C, HAN K, WEI X O, XU Y W, ZHOU X B, ZHANG H, CHEN Z Y. Biosens. Bioelectron., 2020, 154:112091.

    22. [22]

      GE L, WANG W X, SUN X M, HOU T, LI F. Anal. Chem., 2016, 88(4):2212-2219.GE L, WANG W X, SUN X M, HOU T, LI F. Anal. Chem., 2016, 88(4):2212-2219.

    23. [23]

      MIAO J C, DU K, LI X, XU X T, DONG X, FANG J L, CAO W, WEI Q. Biosens. Bioelectron., 2021, 171:112713.MIAO J C, DU K, LI X, XU X T, DONG X, FANG J L, CAO W, WEI Q. Biosens. Bioelectron., 2021, 171:112713.

    24. [24]

      CUI L, LU M F, LI Y, TANG B, ZHANG C Y. Biosens. Bioelectron., 2018, 102:87-93.CUI L, LU M F, LI Y, TANG B, ZHANG C Y. Biosens. Bioelectron., 2018, 102:87-93.

    25. [25]

      LI Y R, CHANG Y Y, MA J, WU Z Y, YUAN R, CHAI Y Q. Anal. Chem., 2019, 91(9):6127-6133.LI Y R, CHANG Y Y, MA J, WU Z Y, YUAN R, CHAI Y Q. Anal. Chem., 2019, 91(9):6127-6133.

    26. [26]

      HU R, ZHANG X, CHI K N, YANG T, YANG Y H. ACS Appl. Mater. Interfaces, 2020, 12(27):30770-30778.HU R, ZHANG X, CHI K N, YANG T, YANG Y H. ACS Appl. Mater. Interfaces, 2020, 12(27):30770-30778.

    27. [27]

      LI J, LIU Y L, ZHU X Q, CHANG G, HE H P, ZHANG X H, WANG S F. ACS Appl. Mater. Interfaces, 2017, 9(50):44231-44240.LI J, LIU Y L, ZHU X Q, CHANG G, HE H P, ZHANG X H, WANG S F. ACS Appl. Mater. Interfaces, 2017, 9(50):44231-44240.

    28. [28]

      LIU Y L, GE Z Q, CHEN M J, HE H P, ZHANG X H, WANG S F. Biosens. Bioelectron., 2019, 142:111537.LIU Y L, GE Z Q, CHEN M J, HE H P, ZHANG X H, WANG S F. Biosens. Bioelectron., 2019, 142:111537.

    29. [29]

      JIN H, GUI R J, YU J B, LV W, WANG Z H. Biosens. Bioelectron., 2017, 91:523-537.JIN H, GUI R J, YU J B, LV W, WANG Z H. Biosens. Bioelectron., 2017, 91:523-537.

    30. [30]

      ZHAO C Q, JIN H, GUI R J, WANG Z H. Sens. Actuators, B, 2017, 242:71-78.ZHAO C Q, JIN H, GUI R J, WANG Z H. Sens. Actuators, B, 2017, 242:71-78.

    31. [31]

      YU J B, JIN H, GUI R J, WANG Z H, GE F. Talanta, 2017, 162:435-439.YU J B, JIN H, GUI R J, WANG Z H, GE F. Talanta, 2017, 162:435-439.

    32. [32]

      JIANG J, WU H Y, SU Y, LIANG Y, SHU B W, ZHANG C S. Anal. Chem., 2020, 92(11):7708-7716.JIANG J, WU H Y, SU Y, LIANG Y, SHU B W, ZHANG C S. Anal. Chem., 2020, 92(11):7708-7716.

    33. [33]

      YUAN Y L, HU T, ZHONG X, ZHU M H, CHAI Y Q, YUAN R. ACS Appl. Mater. Interfaces, 2020, 12(20):22624-22629.YUAN Y L, HU T, ZHONG X, ZHU M H, CHAI Y Q, YUAN R. ACS Appl. Mater. Interfaces, 2020, 12(20):22624-22629.

    34. [34]

      GUO Q Q, YU Y Q, ZHANG H, CAI C X, SHEN Q M. Anal. Chem., 2020, 92(7):5302-5310.GUO Q Q, YU Y Q, ZHANG H, CAI C X, SHEN Q M. Anal. Chem., 2020, 92(7):5302-5310.

    35. [35]

      WANG J R, XIA C, YANG L, LI Y F, LI C M, HUANG C Z. Anal. Chem., 2020, 92(5):4046-4052.WANG J R, XIA C, YANG L, LI Y F, LI C M, HUANG C Z. Anal. Chem., 2020, 92(5):4046-4052.

    36. [36]

      BI S, YUE S Z, ZHANG S S. Chem. Soc. Rev., 2017, 46(14):4281-4298.BI S, YUE S Z, ZHANG S S. Chem. Soc. Rev., 2017, 46(14):4281-4298.

    37. [37]

      FENG C R, ZHANG C, GUO J X, LI G P, YE B X, ZOU L N. Anal. Chim. Acta, 2021, 1158:338413.FENG C R, ZHANG C, GUO J X, LI G P, YE B X, ZOU L N. Anal. Chim. Acta, 2021, 1158:338413.

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  • 收稿日期:  2021-04-30
  • 修回日期:  2022-01-28
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