基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析

韩丹丹 张金凤 邓吉楠 胡宁 杨军

引用本文: 韩丹丹, 张金凤, 邓吉楠, 胡宁, 杨军. 基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析[J]. 分析化学, 2021, 49(10): 1678-1685. doi: 10.19756/j.issn.0253-3820.210443 shu
Citation:  HAN Dan-Dan,  ZHANG Jin-Feng,  DENG Ji-Nan,  HU Ning,  YANG Jun. Preparation and Sensing Studies of Flow Focusing Microfluidic Chip-based Liquid Crystal Droplet[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1678-1685. doi: 10.19756/j.issn.0253-3820.210443 shu

基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析

    通讯作者: 邓吉楠,E-mail:biojdeng@cqu.edu.cn; 杨军,E-mail:bioyangjun@cqu.edu.cn
  • 基金项目:

    国家自然科学基金项目(No.81871450)和中央高校基本科研业务费项目(No.2020CDJQY-A058)资助。

摘要: 针对限制液晶微滴生物传感响应性能的微滴尺寸不均一的问题,设计了流动聚焦微流控芯片结构,系统研究了单分散液晶微滴光学探针的制备方法及传感响应特性。以向列型液晶为分散相,十二烷基硫酸钠/聚乙烯醇混合液为连续相,通过控制流动聚焦时两相流速来调节影响液晶微滴生成的不同流动模式。测试并分析了两相流速、连续相粘度、孔口宽度等参数对液晶微滴尺寸的影响及其内在机制。利用不同尺寸的液晶微滴为传感探针,检测并分析了肝肠疾病的潜在标志物脱氧胆酸。结果表明,当液晶微滴尺寸从38 μm增加到77 μm时,脱氧胆酸的检出限从(383±23.6)μmol/L降低到(140±14.1)μmol/L。

English


    1. [1]

      KHAN M, PARK S Y. Colloids Surf., B, 2015, 127:241-246.KHAN M, PARK S Y. Colloids Surf., B, 2015, 127:241-246.

    2. [2]

      JIN Y, LEE W, MUSINA Z, DING Y L. Particuology, 2010, 8(6):588-590.JIN Y, LEE W, MUSINA Z, DING Y L. Particuology, 2010, 8(6):588-590.

    3. [3]

      ZHOU L, HU Q, KANG Q, YU L. Talanta, 2018, 190:375-381.ZHOU L, HU Q, KANG Q, YU L. Talanta, 2018, 190:375-381.

    4. [4]

      HAN G R, JANG C H. Talanta, 2014, 128:44-50.HAN G R, JANG C H. Talanta, 2014, 128:44-50.

    5. [5]

      LEE K, GUPTA K C, PARK S Y, KANG I K. J. Mater. Chem. B, 2016, 4(4):704-715.LEE K, GUPTA K C, PARK S Y, KANG I K. J. Mater. Chem. B, 2016, 4(4):704-715.

    6. [6]

      KIM J, KHAN M, PARK S Y. ACS Appl. Mater. Interfaces, 2013, 5(24):13135-13139.KIM J, KHAN M, PARK S Y. ACS Appl. Mater. Interfaces, 2013, 5(24):13135-13139.

    7. [7]

      HAN X W, HAN D D, ZENG J Y, DENG J N, HU N, YANG J. Microchem. J., 2020, 157:105057.HAN X W, HAN D D, ZENG J Y, DENG J N, HU N, YANG J. Microchem. J., 2020, 157:105057.

    8. [8]

      DUAN R, LI Y, SHI B, LI H, YANG J. Talanta, 2020, 209:120513.DUAN R, LI Y, SHI B, LI H, YANG J. Talanta, 2020, 209:120513.

    9. [9]

      MILLER D S, ABBOTT N L. Soft Matter, 2013, 9(2):374-382.MILLER D S, ABBOTT N L. Soft Matter, 2013, 9(2):374-382.

    10. [10]

      ZOU J, BERA T, DAVIS A A, LIANG W, FANG J. J. Phys. Chem. B, 2011, 115(29):8970-8974.ZOU J, BERA T, DAVIS A A, LIANG W, FANG J. J. Phys. Chem. B, 2011, 115(29):8970-8974.

    11. [11]

      GUPTA J K, ZIMMERMAN J S, DE PABLO J J, CARUSO F, ABBOTT N L. Langmuir, 2009, 25(16):9016-9024.GUPTA J K, ZIMMERMAN J S, DE PABLO J J, CARUSO F, ABBOTT N L. Langmuir, 2009, 25(16):9016-9024.

    12. [12]

      BAO P, PATERSON D A, HARRISON P L, MILLER K, PEYMAN S, JONES J C, SANDOE J, EVANS S D, BUSHBY R J, GLEESON H F. Lab Chip, 2019, 19(6):1082-1089.BAO P, PATERSON D A, HARRISON P L, MILLER K, PEYMAN S, JONES J C, SANDOE J, EVANS S D, BUSHBY R J, GLEESON H F. Lab Chip, 2019, 19(6):1082-1089.

    13. [13]

      CUBAUD T. Phys. Rev. Fluids, 2019, 4(8):084201.CUBAUD T. Phys. Rev. Fluids, 2019, 4(8):084201.

    14. [14]

      HAN Xian-Wei, ZHANG Hong-Wu, LUO Hong-Yan, ZHENG Xiao-Lin, YANG Zhong, HU Ning, LIAO Yan-Jian, YANG Jun. Chin. J.Anal. Chem., 2018, 46(8):1269-1274. 韩县伟, 张洪武, 罗洪艳, 郑小林, 杨忠, 胡宁, 廖彦剑, 杨军. 分析化学, 2018, 46(8):1269-1274.

    15. [15]

      KHAN W, CHOI J H, KIM G M, PARK S Y. Lab Chip, 2011, 11(20):3493-3498.KHAN W, CHOI J H, KIM G M, PARK S Y. Lab Chip, 2011, 11(20):3493-3498.

    16. [16]

      LASHKARIPOUR A, RODRIGUEZ C, ORTIZ L, DENSMORE D. Lab Chip, 2019, 19(6):1041-1053.LASHKARIPOUR A, RODRIGUEZ C, ORTIZ L, DENSMORE D. Lab Chip, 2019, 19(6):1041-1053.

    17. [17]

      KHAN W, PARK S Y. Lab Chip, 2012, 12(21):4553-4559.KHAN W, PARK S Y. Lab Chip, 2012, 12(21):4553-4559.

    18. [18]

      NISISAKO T, OKUSHIMA S, TORII T. Soft Matter, 2005, 1(1):23-27.NISISAKO T, OKUSHIMA S, TORII T. Soft Matter, 2005, 1(1):23-27.

    19. [19]

      OWEN M J, SMITH P J. J. Adhes. Sci. Technol., 1994, 8(10):1063-1075.OWEN M J, SMITH P J. J. Adhes. Sci. Technol., 1994, 8(10):1063-1075.

    20. [20]

      JUNG Y D, KHAN M, PARK S Y. J. Mater. Chem. B, 2014, 2(30):4922-4928.JUNG Y D, KHAN M, PARK S Y. J. Mater. Chem. B, 2014, 2(30):4922-4928.

    21. [21]

      JANG J, PARK S. Sens. Actuators, B, 2017, 241:636-643.JANG J, PARK S. Sens. Actuators, B, 2017, 241:636-643.

    22. [22]

      KWON J, KHAN M, PARK S. RSC Adv., 2016, 6(61):55976-55983.KWON J, KHAN M, PARK S. RSC Adv., 2016, 6(61):55976-55983.

    23. [23]

      CAO Z, WU Z, SUNDÉN B. Chem. Eng. J., 2018, 344:604-615.CAO Z, WU Z, SUNDÉN B. Chem. Eng. J., 2018, 344:604-615.

    24. [24]

      WU Z, CAO Z, SUNDÉN B. Chem. Eng. Sci., 2017, 174:56-66.WU Z, CAO Z, SUNDÉN B. Chem. Eng. Sci., 2017, 174:56-66.

    25. [25]

      UTADA A S, LORENCEAU E, LINK D R, KAPLAN P D, STONE H A, WEITZ D A. Science, 2005,308(5721):537-541.UTADA A S, LORENCEAU E, LINK D R, KAPLAN P D, STONE H A, WEITZ D A. Science, 2005,308(5721):537-541.

    26. [26]

      DU J P, IBASETA N, GUICHARDON P. Chem. Eng. Res. Des., 2020, 159:615-627.DU J P, IBASETA N, GUICHARDON P. Chem. Eng. Res. Des., 2020, 159:615-627.

    27. [27]

      LIANG D, MA P C, ZHU C Y, FU T T, MA Y G, WANG K, LUO G S. Ind. Eng. Chem. Res., 2019, 58(41):19226-19238.LIANG D, MA P C, ZHU C Y, FU T T, MA Y G, WANG K, LUO G S. Ind. Eng. Chem. Res., 2019, 58(41):19226-19238.

    28. [28]

      CRAMER C, FISCHER P, WINDHAB E J. Chem. Eng. Sci., 2004, 59(15):3045-3058.CRAMER C, FISCHER P, WINDHAB E J. Chem. Eng. Sci., 2004, 59(15):3045-3058.

    29. [29]

      SONTTI S G, ATTA A. Ind. Eng. Chem. Res., 2019, 59(9):3702-3716.SONTTI S G, ATTA A. Ind. Eng. Chem. Res., 2019, 59(9):3702-3716.

    30. [30]

      NUNES J K, TSAI S S, WAN J, STONE H A. J. Phys. D:Appl. Phys., 2013, 46(11):114002.NUNES J K, TSAI S S, WAN J, STONE H A. J. Phys. D:Appl. Phys., 2013, 46(11):114002.

    31. [31]

      LIU H H, ZHANG Y H. Phys. Fluids, 2011, 23(8):082101.LIU H H, ZHANG Y H. Phys. Fluids, 2011, 23(8):082101.

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  • 收稿日期:  2021-04-16
  • 修回日期:  2021-08-11
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