基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析

引用本文: 韩丹丹, 张金凤, 邓吉楠, 胡宁, 杨军. 基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析[J]. 分析化学, 2021, 49(10): 1678-1685. doi: 10.19756/j.issn.0253-3820.210443 shu

Citation: HAN Dan-Dan, ZHANG Jin-Feng, DENG Ji-Nan, HU Ning, YANG Jun. Preparation and Sensing Studies of Flow Focusing Microfluidic Chip-based Liquid Crystal Droplet[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1678-1685. doi: 10.19756/j.issn.0253-3820.210443 shu

基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析

重庆大学生物工程学院, 生物流变科学与技术教育部重点实验室, 重庆 400030

通讯作者: 邓吉楠,E-mail:biojdeng@cqu.edu.cn; 杨军,E-mail:bioyangjun@cqu.edu.cn

基金项目:
国家自然科学基金项目（No.81871450）和中央高校基本科研业务费项目（No.2020CDJQY-A058）资助。

摘要: 针对限制液晶微滴生物传感响应性能的微滴尺寸不均一的问题，设计了流动聚焦微流控芯片结构，系统研究了单分散液晶微滴光学探针的制备方法及传感响应特性。以向列型液晶为分散相，十二烷基硫酸钠/聚乙烯醇混合液为连续相，通过控制流动聚焦时两相流速来调节影响液晶微滴生成的不同流动模式。测试并分析了两相流速、连续相粘度、孔口宽度等参数对液晶微滴尺寸的影响及其内在机制。利用不同尺寸的液晶微滴为传感探针，检测并分析了肝肠疾病的潜在标志物脱氧胆酸。结果表明，当液晶微滴尺寸从38 μm增加到77 μm时，脱氧胆酸的检出限从（383±23.6）μmol/L降低到（140±14.1）μmol/L。

关键词:

微流控
/ 液晶微滴
/ 聚焦流
/ 传感
/ 两相流

English

Preparation and Sensing Studies of Flow Focusing Microfluidic Chip-based Liquid Crystal Droplet

Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China

Corresponding author: DENG Ji-Nan, ; YANG Jun,

Received Date: 16 April 2021
Revised Date: 11 August 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.81871450) and the Fundamental Research Funds for the Central Universities, China (No.2020CDJQY-A058).

Abstract: Liquid crystal (LC) droplets are promising for biosensor applications. However, the nonuniform size of LC droplets has limited their sensing performance. In this work, the generation of monodispersed LC droplets using a flow-focusing microfluidic chip was systematically investigated. With the nematic liquid crystal as the dispersed phase and sodium dodecyl sulfate aqueous/polyvinyl alcohol solution as the continuous phase, different flow patterns for the generation of LC droplet were studied. The effects of flow rates of the two phases, the viscosity of the continuous phase and the width of the orifice on the size of the generated LC droplets were also investigated. Finally, the size effect of the LC droplets on the sensing performance for deoxycholic acid (DCA), a potential biomarker of liver and intestinal diseases, was preliminarily explored. The results showed that the detection limit of LC droplets for DCA decreased from (383 ±23.6) μmol/L to (140 ±14.1) μmol/L when the diameter of LC droplets increased from 38 μm to 77 μm.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析

通讯作者: 邓吉楠,E-mail:biojdeng@cqu.edu.cn; 杨军,E-mail:bioyangjun@cqu.edu.cn

English

Preparation and Sensing Studies of Flow Focusing Microfluidic Chip-based Liquid Crystal Droplet

Corresponding author: DENG Ji-Nan, ; YANG Jun,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于流动聚焦微流控芯片的液晶微滴制备及传感响应分析

通讯作者: 邓吉楠,E-mail:biojdeng@cqu.edu.cn; 杨军,E-mail:bioyangjun@cqu.edu.cn

English

Preparation and Sensing Studies of Flow Focusing Microfluidic Chip-based Liquid Crystal Droplet

Corresponding author: DENG Ji-Nan, ; YANG Jun,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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