N-糖组富集方法研究进展

引用本文: 熊莹莹, 程孟霞, 陆豪杰. N-糖组富集方法研究进展[J]. 分析化学, 2021, 49(10): 1597-1606. doi: 10.19756/j.issn.0253-3820.210427 shu

Citation: XIONG Ying-Ying, CHENG Meng-Xia, LU Hao-Jie. Research Progress of N-Glycome Enrichment Methods[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1597-1606. doi: 10.19756/j.issn.0253-3820.210427 shu

N-糖组富集方法研究进展

复旦大学化学系和生物医学研究院, 国家卫生健康委员会糖复合物重点实验室(复旦大学), 上海 200032

通讯作者: 陆豪杰,E-mail:luhaojie@fudan.edu.cn

基金项目:
国家重点研发计划项目（Nos.2016YFA0501303，2017YFA0505001）和国家自然科学基金项目（No.21974025）资助。

摘要: 糖基化是一种重要的蛋白质翻译后修饰，使蛋白质具有更多样的功能。N-糖基化作为蛋白质主要的糖基化类型之一，与许多生命活动密切相关，在疾病发生发展中起关键作用。因此，研究糖蛋白N-糖链具有重要意义。生物质谱技术是糖组学研究的重要工具之一，然而，由于糖链结构复杂、丰度低及质谱离子化效率低等原因，基于质谱的N-糖组学研究还面临着很大的挑战。采用生物质谱对N-糖链进行分析时，N-糖链的分离富集必不可少。本文简要介绍了基于质谱的N-糖链分析流程，重点综述了糖链分离富集方法，总结了不同方法的优缺点以及各种技术在生物医学研究中的应用。

关键词:

糖基化
/ N-糖链
/ 糖组学
/ 分离富集
/ 质谱
/ 评述

English

Research Progress of N-Glycome Enrichment Methods

NHC Key Laboratory of Glycoconjugates Research(Fudan University), Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China

Corresponding author: LU Hao-Jie, luhaojie@fudan.edu.cn

Received Date: 09 April 2021
Revised Date: 10 August 2021
Fund Project:
Supported by the Natural National Key Research and Development Program (Nos.2016YFA0501303, 2017YFA0505001) and the National Natural Science Foundation of China (No.21974025).

Abstract: Glycosylation is an important post-translational modification of proteins. Modification by glycans makes the functions of proteins diverse. As one of the main glycosylation types of proteins, N-glycosylation is closely involved in many life activities and plays a critical role in the occurrence and development of diseases. Therefore, it is of great significance to study the N-glycans on glycoproteins. Mass spectrometry technology is one of the most powerful tools for studying N-glycome. However, due to the complex structure, low abundance and low ionization efficiency of glycans in mass spectrometry, the research of N-glycome based on mass spectrometry still faces great challenges. The separation and enrichment of N-glycans is essential for the analysis of N-glycans by mass spectrometry. This article briefly introduces the process of N-glycan analysis based on mass spectrometry, focusing on the overview of N-glycan separation and enrichment methods, and summarizes the advantages and disadvantages of these methods and discusses the application and contribution of these various technologies in biomedical research.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

N-糖组富集方法研究进展

通讯作者: 陆豪杰,E-mail:luhaojie@fudan.edu.cn

English

Research Progress of N-Glycome Enrichment Methods

Corresponding author: LU Hao-Jie, luhaojie@fudan.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

N-糖组富集方法研究进展

通讯作者: 陆豪杰,E-mail:luhaojie@fudan.edu.cn

English

Research Progress of N-Glycome Enrichment Methods

Corresponding author: LU Hao-Jie, luhaojie@fudan.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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