体外血脑屏障的构建及金属离子/金属纳米粒子透过性评价

姜泽 张璇 刘金辉 魏星 陈明丽 王建华

引用本文: 姜泽, 张璇, 刘金辉, 魏星, 陈明丽, 王建华. 体外血脑屏障的构建及金属离子/金属纳米粒子透过性评价[J]. 分析化学, 2021, 49(10): 1657-1665. doi: 10.19756/j.issn.0253-3820.210406 shu
Citation:  JIANG Ze,  ZHANG Xuan,  LIU Jin-Hui,  WEI Xing,  CHEN Ming-Li,  WANG Jian-Hua. Construction of Human Blood-Brain Barrier in Vitro and Permeability Evaluation of Metal Ions or Metal Nanoparticles[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1657-1665. doi: 10.19756/j.issn.0253-3820.210406 shu

体外血脑屏障的构建及金属离子/金属纳米粒子透过性评价

    通讯作者: 陈明丽,E-mail:chenml@mail.neu.edu.cn; 王建华,E-mail:jianhuajrz@mail.neu.edu.cn
  • 基金项目:

    国家自然科学基金项目(Nos.21727811,21922402)、中央高校基本科研业务费专项资金项目(Nos.N2005003,N2105017)、辽宁省兴辽英才计划项目(No.XLYC1802016)和辽宁省高校创新人才支持计划项目(No.ZX20200088)资助。

摘要: 构建了一种人体外血脑屏障微流控芯片用于评估金属和金属纳米粒子的透过性。结合"三明治"血脑屏障模型和集成浓度梯度生成单元芯片,实现了不同浓度下金属相关组份的透过评估。利用bEnd.3细胞构建血脑屏障模型,采用电感耦合等离子体质谱(ICP-MS)法对体外血脑屏障模型中金属离子和金属纳米粒子的跨膜效应和屏障吸收定量检测。通过Comsol模拟软件对芯片浓度梯度生成单元混合效果进行理论模拟,采用罗丹明B溶液和含镉的实际样品评价了其实际混合效果,采用原子吸收光谱评估了其可靠性。屏障渗透性评估实验结果表明,荧光素钠的血脑屏障渗透率为(5.45±0.48)×10-6 cm/s,与文献报道值基本一致。此芯片能够实现稳定的浓度生成、体外血脑屏障器官的模拟以及其对金属溶液透过性的评估,有望用于中枢神经药物筛选和金属、纳米粒子神经毒性评估等领域。

English


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  • 收稿日期:  2021-04-01
  • 修回日期:  2021-08-16
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