基于DNA步行器信号放大的便携式传感器检测铅离子

引用本文: 苏永欢, 蔡杰, 杨雅妮, 刘冰倩. 基于DNA步行器信号放大的便携式传感器检测铅离子[J]. 分析化学, 2022, 50(4): 525-534. doi: 10.19756/j.issn.0253-3820.201787 shu

Citation: SU Yong-huan, CAI Jie, YANG Ya-ni, LIU Bing-qian. A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 525-534. doi: 10.19756/j.issn.0253-3820.201787 shu

基于DNA步行器信号放大的便携式传感器检测铅离子

贵州大学药学院, 贵州省合成药物工程实验室, 贵阳 550025

通讯作者: 刘冰倩,E-mail:nqliu@gzu.edu.cn

基金项目:
国家自然科学基金项目(Nos.21864007,21605029)

贵州省自然科学基金项目(Nos.黔科合基础[2020] 1042,黔科合平台人才[2017] 5788,黔科合平台人才[2018] 5781)资助

摘要: 基于Pb²⁺与底物链(Substrate DNA, sDNA)的特异性识别,以Fe₃O₄纳米磁珠(Fe₃O₄ nano-magnetic beads, MB)为基底,便携式血糖仪(Portable glucose meter, PGM)为信号读出系统,利用DNA步行器放大信号,构建了一种灵敏且便捷的用于铅离子(Pb²⁺)检测的传感器。利用葡萄糖氧化酶(Glucose oxidase, GOx)和sDNA修饰还原法制备纳米金功能化的聚酰胺-胺型(Nanogold-functionalized poly(amidoamine), Au/PAMAM)树枝分子,形成sDNA/GOx/Au/PAMAM复合物;基于碱基互补配对原则,sDNA/GOx/Au/PAMAM复合物被固载于捕获链(Capture DNA, cDNA)修饰的MB(MB/cDNA)上,而DNA酶(DNAzyme)可与sDNA未碱基配对部分结合。当目标物存在时,Pb²⁺可特异性识别s DNA上裂解位点并剪切,释放DNAzyme和GOx/Au/PAMAM。DNAzyme继续与sDNA/GOx/Au/PAMAM复合物上下一个sDNA配对结合,继而开启下一轮识别-剪切-释放,循环往复,形成DNA步行器,增加GOx/Au/PAMAM释放量,实现信号放大。反应体系中Pb²⁺浓度越高,释放的GOx/Au/PAMAM量越多,通过磁性分离取上清液(GOx/Au/PAMAM)催化反应液中的葡萄糖(Glucose, Glu)氧化,使葡萄糖的浓度降低。用PGM测定Glu浓度,从而实现对Pb²⁺浓度的间接测定。在最佳实验条件下,体系对PGM的响应值与Pb²⁺浓度的对数呈反比,线性范围为1.00 pmol/L~1.00μmol/L,检出限为0.66 pmol/L。

关键词:

English

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺

Synthetic Pharmaceutical Engineering Laboratory of Guizhou Province, School of Pharmacy, Guizhou University, Guiyang 550025, China

Corresponding author: LIU Bing-qian, nqliu@gzu.edu.cn

Received Date: 24 December 2020
Revised Date: 22 February 2022
Fund Project:
Supported by the National Natural Science Foundation of China(Nos. 21864007, 21605029)

the Guizhou Provincial Natural Science Foundation(Nos. Qian Ke He Ji Chu [2020]1Y042, Qian Ke He Platform for Talents [2017]5788, Qian Ke He Platform for Talents [2018]5781)

Abstract: A sensitive and convenient sensor for detection of Pb²⁺ was established based on the specific recognition of Pb²⁺ and substrate DNA(sDNA), coupling with Fe₃O₄ nano-magnetic beads(MB) as sensor platform, portable glucose meter(PGM) as signal capture terminal and DNA walking device as signal amplification strategy. The nanogold-functionalized Poly(amidoamine() Au/PAMAM) dendrimers prepared by reduction method were modified by glucose oxidase(GOx) and sDNA to form sDNA/GOx/Au/PAMAM complex. Based on the complementary base pairing principle, the sDNA/GOx/Au/PAMAM complex was captured on the MB modified by capture DNA(c DNA), and the DNA enzymes(DNAzyme) could bind to the un-paired parts of sDNA. In the presence of target, Pb²⁺ could specifically recognize cleavage sites on sDNA and shear it, meanwhile released DNAzyme and GOx/Au/PAMAM. The former continued to bind with the next sDNA, and then the new identification-split-release circulation was opened.The circulation could be called DNA walker device, and the release quantity of GOx/Au/PAMAM was increased to realize signal amplification. The supernatant(GOx/Au/PAMAM), separated by magnetic separation, could oxidize glucose(Glu) in the reaction solution, and the change value of Glu concentration was monitored with PGM, thus indirectly detecting the target Pb²⁺ concentration. Under the optimal conditions, the PGM response of the system showed a good linear relationship with the logarithm of Pb²⁺ concentration ranging from 1.00 pmol/L to 1.00 μmol/L, and the detection limit was 0.66 pmol/L.

Key words:

1. [1]
  HE W, LUO L, LIU Q, CHEN Z. Anal. Chem., 2018, 90(7):4770-4775.HE W, LUO L, LIU Q, CHEN Z. Anal. Chem., 2018, 90(7):4770-4775.
2. [2]
  LIN W, LI Z, BURNS M. Anal. Chem., 2017, 89(17):8748-8756.LIN W, LI Z, BURNS M. Anal. Chem., 2017, 89(17):8748-8756.
3. [3]
  CHEN C, HUANG W. J. Am. Chem. Soc., 2002, 124(22):6246-6247.CHEN C, HUANG W. J. Am. Chem. Soc., 2002, 124(22):6246-6247.
4. [4]
  KAVALLIERATOS K, ROSENBERG J, CHEN W, REN T. J. Am. Chem. Soc., 2005, 127(18):6514-6515.KAVALLIERATOS K, ROSENBERG J, CHEN W, REN T. J. Am. Chem. Soc., 2005, 127(18):6514-6515.
5. [5]
  DUAN N, LI C, SONG M, WANG Z, ZHU C, WU S. Spectrochim. Acta, Part A, 2022, 265(15):120342.DUAN N, LI C, SONG M, WANG Z, ZHU C, WU S. Spectrochim. Acta, Part A, 2022, 265(15):120342.
6. [6]
  BABU S, KUMAR K, SUVARDHAN K, KIRAN K, REKHA D, KRISHNAIAH L, JANARDHANAM K,CHIRANJEEVI P. Environ. Monit. Assess., 2007, 128:241-249.BABU S, KUMAR K, SUVARDHAN K, KIRAN K, REKHA D, KRISHNAIAH L, JANARDHANAM K,CHIRANJEEVI P. Environ. Monit. Assess., 2007, 128:241-249.
7. [7]
  COCHERIE A, ROBERT M. Chem. Geol., 2007, 243:90-104.COCHERIE A, ROBERT M. Chem. Geol., 2007, 243:90-104.
8. [8]
  MORTADA W I, KENAWY I M, ABDELGHANY A M, ISMAL A M, DONIA A F, NABIEH K A. Mater. Sci. Eng.C, 2015, 52(1):288-296.MORTADA W I, KENAWY I M, ABDELGHANY A M, ISMAL A M, DONIA A F, NABIEH K A. Mater. Sci. Eng.C, 2015, 52(1):288-296.
9. [9]
  LIU S, SUN J, HUO J, DUAN X, LIU Y. Anal. Sci., 2019, 35(5):499-504.LIU S, SUN J, HUO J, DUAN X, LIU Y. Anal. Sci., 2019, 35(5):499-504.
10. [10]
  SUN Q, WANG J, TANG M, HUANG L, ZHANG Z, LIU C, LU X, HUNTER K W, CHEN G. Anal. Chem., 2017,89(9):5024-5029.SUN Q, WANG J, TANG M, HUANG L, ZHANG Z, LIU C, LU X, HUNTER K W, CHEN G. Anal. Chem., 2017,89(9):5024-5029.
11. [11]
  LIU Tao, LI Dan, LIANG Jie, WANG Xiu-Mei. Chin. J. Anal. Chem., 2020, 48(2):248-254.刘涛,李丹,梁杰,汪秀妹.分析化学, 2020, 48(2):248-254.
12. [12]
  MALIK L, BASHIR A, QUREASHI A, PANDITH A. Environ. Chem. Lett., 2019, 17:1495-1521.MALIK L, BASHIR A, QUREASHI A, PANDITH A. Environ. Chem. Lett., 2019, 17:1495-1521.
13. [13]
  ZHAO G, LIU G. IEEE Sens. J., 2018, 18(14):5645-5655.ZHAO G, LIU G. IEEE Sens. J., 2018, 18(14):5645-5655.
14. [14]
  LU W, LIN C, YANG J, WANG X, YAO B, WANG M. Anal. Bioanal. Chem., 2019, 411(21):5383-5391.LU W, LIN C, YANG J, WANG X, YAO B, WANG M. Anal. Bioanal. Chem., 2019, 411(21):5383-5391.
15. [15]
  TANG D, TANG J, SU B, CHEN G. Biosens. Bioelectron., 2011, 26(5):2090-2096.TANG D, TANG J, SU B, CHEN G. Biosens. Bioelectron., 2011, 26(5):2090-2096.
16. [16]
  TANG J, HUANG Y, ZHANG C, LIU H, TANG D. Biosens. Bioelectron., 2016, 86:386-392.TANG J, HUANG Y, ZHANG C, LIU H, TANG D. Biosens. Bioelectron., 2016, 86:386-392.
17. [17]
  YUAN Y, ZHANG G, LI Y, ZHANG G, ZHANG F, FAN X. Polym. Chem., 2013, 4(6):2164-2167.YUAN Y, ZHANG G, LI Y, ZHANG G, ZHANG F, FAN X. Polym. Chem., 2013, 4(6):2164-2167.
18. [18]
  QIU Z, TANG D, SHU J, CHEN G, TANG D. Biosens. Bioelectron., 2016, 75:108-115.QIU Z, TANG D, SHU J, CHEN G, TANG D. Biosens. Bioelectron., 2016, 75:108-115.
19. [19]
  AN Y, JIANG X, BI W, CHEN H, JIN L, ZHANG S, WANG C, ZHANG W. Biosens. Bioelectron., 2012, 32(1):224-230.AN Y, JIANG X, BI W, CHEN H, JIN L, ZHANG S, WANG C, ZHANG W. Biosens. Bioelectron., 2012, 32(1):224-230.
20. [20]
  HOU Y, WANG J, JIANG Y, LV C, XIA L, HONG S, LIN M, LIN Y, ZHANG Z, PANG D. Biosens. Bioelectron.,2018, 99:186-192.HOU Y, WANG J, JIANG Y, LV C, XIA L, HONG S, LIN M, LIN Y, ZHANG Z, PANG D. Biosens. Bioelectron.,2018, 99:186-192.
21. [21]
  WANG W, LI J, DONG C, LI Y, KOU Q, YAN J, ZHANG L. Anal. Chim. Acta, 2018, 1042(26):116-124.WANG W, LI J, DONG C, LI Y, KOU Q, YAN J, ZHANG L. Anal. Chim. Acta, 2018, 1042(26):116-124.
22. [22]
  SHU B, ZHANG C, XING D. Biosens. Bioelectron., 2017, 97:360-368.SHU B, ZHANG C, XING D. Biosens. Bioelectron., 2017, 97:360-368.
23. [23]
  WEI M, WANG C, XU E, CHEN J, XU X, WEI W, LIU S. Food Chem., 2019, 282(1):141-146.WEI M, WANG C, XU E, CHEN J, XU X, WEI W, LIU S. Food Chem., 2019, 282(1):141-146.
24. [24]
  LIU J, HU Q, QI L, LIN J, LI Y. J. Hazard. Mater., 2020, 400(5):123218.LIU J, HU Q, QI L, LIN J, LI Y. J. Hazard. Mater., 2020, 400(5):123218.
25. [25]
  WANG K, HE M, ZHAI F, WANG J, HE R, YU Y. Biosens. Bioelectron., 2018, 105:159-165.WANG K, HE M, ZHAI F, WANG J, HE R, YU Y. Biosens. Bioelectron., 2018, 105:159-165.
26. [26]
  YANG F, ZHONG X, JIANG X, ZHUO Y, YUAN R, WEI S. Biosens. Bioelectron., 2019, 130:262-268.YANG F, ZHONG X, JIANG X, ZHUO Y, YUAN R, WEI S. Biosens. Bioelectron., 2019, 130:262-268.
27. [27]
  CHANG Y, WU Z, SUN Q, ZHUO Y, CHAI Y, YUAN R. Anal. Chem., 2019, 91(13):8123-8128.CHANG Y, WU Z, SUN Q, ZHUO Y, CHAI Y, YUAN R. Anal. Chem., 2019, 91(13):8123-8128.
28. [28]
  LIU X, NIAZOV-ELKAN A, WANG F, WILLNER I. Nano Lett., 2013, 13(1):219-225.LIU X, NIAZOV-ELKAN A, WANG F, WILLNER I. Nano Lett., 2013, 13(1):219-225.
29. [29]
  DELIUS D, GEERTSEMA E M, LEIGH D A, TANG D D. J. Am. Chem. Soc., 2010, 132(45):16134-16145.DELIUS D, GEERTSEMA E M, LEIGH D A, TANG D D. J. Am. Chem. Soc., 2010, 132(45):16134-16145.
30. [30]
  WANG C, REN J, QU X. Chem. Commun., 2011, 47(5):1428-1430.WANG C, REN J, QU X. Chem. Commun., 2011, 47(5):1428-1430.
31. [31]
  XU Q, ZHANG Y, ZHANG C. Chem. Commun., 2015, 51(26):5652-5655.XU Q, ZHANG Y, ZHANG C. Chem. Commun., 2015, 51(26):5652-5655.
32. [32]
  WANG Y, SONG W, ZHAO H, MA X, YANG S, QIAO X, SHENG Q, YUE T. Biosens. Bioelectron., 2021, 182:113171.WANG Y, SONG W, ZHAO H, MA X, YANG S, QIAO X, SHENG Q, YUE T. Biosens. Bioelectron., 2021, 182:113171.
33. [33]
  YANG X, SHI D, ZHU S, WANG B, ZHANG X, WANG G. ACS Sens., 2018, 3(7):1368-1375.YANG X, SHI D, ZHU S, WANG B, ZHANG X, WANG G. ACS Sens., 2018, 3(7):1368-1375.
34. [34]
  ESUMI K, SHINTARO AKIYAMA A, YOSHIMURA T. Langmuir, 2003, 19(18):7679-7681.ESUMI K, SHINTARO AKIYAMA A, YOSHIMURA T. Langmuir, 2003, 19(18):7679-7681.
35. [35]
  SHI Wei-Ping, CAI Jie, YANG Ya-Ni, LUO Huan-Huan, LIU Bing-Qian, FU Qiu-Ping. Chin. J. Anal. Chem.,2019, 47(9):1337-1343.石维平,蔡杰,杨雅妮,罗欢欢,刘冰倩,付秋平.分析化学, 2019, 47(9):1337-1343.
36. [36]
  CAO L, ZHANG Q, DAI H, FU Y, LI Y. Electroanalysis, 2018, 30(3):517-524.CAO L, ZHANG Q, DAI H, FU Y, LI Y. Electroanalysis, 2018, 30(3):517-524.
37. [37]
  FANG J, GUO Y, YANG Y, YU W, TAO Y, DAI T, YUAN C, XIE G. Sens. Actuators, B, 2018, 272(1):118-126FANG J, GUO Y, YANG Y, YU W, TAO Y, DAI T, YUAN C, XIE G. Sens. Actuators, B, 2018, 272(1):118-126
38. [38]
  LU L, SI J, GAO Z, ZHANG Y, LEI J, LUO H, LI N. Biosens. Bioelectron., 2015, 63:14-20.LU L, SI J, GAO Z, ZHANG Y, LEI J, LUO H, LI N. Biosens. Bioelectron., 2015, 63:14-20.
39. [39]
  ZHANG J, TANG Y, TENG L, LU M, TANG D. Biosens. Bioelectron., 2015, 68:232-238.ZHANG J, TANG Y, TENG L, LU M, TANG D. Biosens. Bioelectron., 2015, 68:232-238.
40. [40]
  SU P G, TZOU W H. Sens. Actuators, A, 2012, 179:44-49.SU P G, TZOU W H. Sens. Actuators, A, 2012, 179:44-49.
41. [41]
  SANCHES E A, SOARES J C, IOST R M, MARANGONI V S, TROVATI G, BATISTA T, MAFUD A C,ZUCOLOTTO V, MASCARENHAS Y P. J. Nanomater., 2011, 2011:73-79.SANCHES E A, SOARES J C, IOST R M, MARANGONI V S, TROVATI G, BATISTA T, MAFUD A C,ZUCOLOTTO V, MASCARENHAS Y P. J. Nanomater., 2011, 2011:73-79.
42. [42]
  LIN Y, ZHOU Q, LIN Y, TANG D, NIESSNER R, KNOPP D. Anal. Chem., 2015, 87(16):8531-8540.LIN Y, ZHOU Q, LIN Y, TANG D, NIESSNER R, KNOPP D. Anal. Chem., 2015, 87(16):8531-8540.
43. [43]
  WRIGHT A K, THOMPSON M R. Biophys. J., 1975, 15(2):137-141.WRIGHT A K, THOMPSON M R. Biophys. J., 1975, 15(2):137-141.
44. [44]
  EBRAHIMI A, RAVAN H, MEHRABANI M. Biosens. Bioelectron., 2020, 170:112710.EBRAHIMI A, RAVAN H, MEHRABANI M. Biosens. Bioelectron., 2020, 170:112710.
45. [45]
  LI W, GAO Y, ZHANG J, WANG X, YIN F, LI Z, ZHANG M. Nanoscale Adv., 2020, 2(2):717-723.LI W, GAO Y, ZHANG J, WANG X, YIN F, LI Z, ZHANG M. Nanoscale Adv., 2020, 2(2):717-723.
46. [46]
  LU Zhao, LIU Mao-Shun, GE Nai-Jia, ZHANG Yi-Zhi, REN Yuan, LI Na, ZHANG Yi-Qiong. Chem. Res. Appl.,2020, 32(2):194-201.陆钊,刘茂顺,葛乃嘉,张怡之,任元,李娜,张艺琼.化学研究与应用, 2020, 32(2):194-201.
47. [47]
  FANG X, LIU Y, JIMENEZ L, DUAN Y, ADKINS G B, QIAO L, LIU B, ZHONG W. Anal. Chem., 2017, 89(21):11758-11764.FANG X, LIU Y, JIMENEZ L, DUAN Y, ADKINS G B, QIAO L, LIU B, ZHONG W. Anal. Chem., 2017, 89(21):11758-11764.
48. [48]
  YU Y, YU C, NIU Y, CHEN J, ZHAO Y, ZHANG Y, GAO R, HE J. Biosens. Bioelectron., 2018, 101:297-303.YU Y, YU C, NIU Y, CHEN J, ZHAO Y, ZHANG Y, GAO R, HE J. Biosens. Bioelectron., 2018, 101:297-303.
49. [49]
  TANG W, YU J, WANG Z, JEERAPAN I, YIN L, ZHANG F, HE P. Anal. Chim. Acta, 2019, 1078(31):1078:53-59.TANG W, YU J, WANG Z, JEERAPAN I, YIN L, ZHANG F, HE P. Anal. Chim. Acta, 2019, 1078(31):1078:53-59.
50. [50]
  SHI Y, WANG H, JIANG X, SUN B, SONG B, SU Y, HE Y. Anal. Chem., 2016, 88(7):3723-3729.SHI Y, WANG H, JIANG X, SUN B, SONG B, SU Y, HE Y. Anal. Chem., 2016, 88(7):3723-3729.
51. [51]
  JIANG D, DU X, CHEN D, ZHOU L, CHEN W, LI Y, HAO N, QIAN J, LIU Q, WANG K. Biosens. Bioelectron.,2016, 83:149-155.JIANG D, DU X, CHEN D, ZHOU L, CHEN W, LI Y, HAO N, QIAN J, LIU Q, WANG K. Biosens. Bioelectron.,2016, 83:149-155.
52. [52]
  WANG X, YANG C, ZHU S, YAN M, GE S, YU J. Biosens. Bioelectron., 2017, 87:108-115.WANG X, YANG C, ZHU S, YAN M, GE S, YU J. Biosens. Bioelectron., 2017, 87:108-115.

扫一扫看文章

计量

PDF下载量: 4
文章访问数: 546
HTML全文浏览量: 28

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于DNA步行器信号放大的便携式传感器检测铅离子

通讯作者: 刘冰倩,E-mail:nqliu@gzu.edu.cn

English

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺

Corresponding author: LIU Bing-qian, nqliu@gzu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于DNA步行器信号放大的便携式传感器检测铅离子

通讯作者: 刘冰倩,E-mail:nqliu@gzu.edu.cn

English

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb2+

Corresponding author: LIU Bing-qian, nqliu@gzu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

A Portable Sensor Based on DNA Walker Signal Amplification for Detection of Pb²⁺

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs