靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究

陈雨湉 任一平 王丽丽 黄忠平

引用本文: 陈雨湉, 任一平, 王丽丽, 黄忠平. 靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究[J]. 分析化学, 2021, 49(9): 1523-1530. doi: 10.19756/j.issn.0253-3820.201768 shu
Citation:  CHEN Yu-Tian,  REN Yi-Ping,  WANG Li-Li,  HUANG Zhong-Ping. Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1523-1530. doi: 10.19756/j.issn.0253-3820.201768 shu

靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究

    通讯作者: 王丽丽,E-mail:lili_wang@zjut.edu.cn; 黄忠平,E-mail:zphuang@zjut.edu.cn
摘要: 基于靶向蛋白质组学技术,采用超高效液相色谱-串联高分辨质谱/串联三重四极杆质谱,建立了一种鉴别奶牛、水牛、牦牛、山羊、绵羊、马、驴和骆驼共8个物种奶的分析方法。针对8个物种奶中6种乳蛋白酶解产生的特征多肽进行分析,利用同位素内标法实现多条多肽的同时检测及比较,通过理论多肽分析、实际多肽检测、干扰考察、线性与最大酶解时间比较,最终筛选出13条特征多肽。依据13条特征多肽对模拟混合奶样品中的不同物种奶进行分析,对市售16个奶制品中的奶源物种进行鉴别。结果表明,特征多肽在奶源物种鉴别中具有良好的稳定性与特异性,本方法对多物种奶制品行业的质量控制与监管具有参考价值。

English


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  • 收稿日期:  2020-12-18
  • 修回日期:  2021-04-22
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