基于金纳米颗粒的裂分型适配体传感器检测三磷酸腺苷

任林娇 彭政 孟晓龙 张培 秦自瑞 徐晓萍 徐鹏 姜利英

引用本文: 任林娇, 彭政, 孟晓龙, 张培, 秦自瑞, 徐晓萍, 徐鹏, 姜利英. 基于金纳米颗粒的裂分型适配体传感器检测三磷酸腺苷[J]. 分析化学, 2022, 50(3): 405-412. doi: 10.19756/j.issn.0253-3820.201749 shu
Citation:  REN Lin-Jiao,  PENG Zheng,  MENG Xiao-Long,  ZHANG Pei,  QIN Zi-Rui,  XU Xiao-Ping,  XU Peng,  JIANG Li-Ying. A Split-Aptamer Sensor for Detection of Adenosine Triphosphate Based on Gold Nanoparticles[J]. Chinese Journal of Analytical Chemistry, 2022, 50(3): 405-412. doi: 10.19756/j.issn.0253-3820.201749 shu

基于金纳米颗粒的裂分型适配体传感器检测三磷酸腺苷

    通讯作者: 张培,E-mail:zhangpei@zzuli.edu.cn; 姜利英,E-mail:jiangliying@zzuli.edu.cn
  • 基金项目:

    国家自然科学基金项目(Nos.62073299,61801436)、河南省高校科技创新团队项目(No.20IRTSTHN017)、河南省重点研发与推广项目(No.202102210186)和河南省高等学校重点科研项目(No.21A410001)资助。

摘要: 构建了一种基于金纳米颗粒(Gold nanoparticles,AuNPs)的裂分型适配体荧光传感器,用于检测三磷酸腺苷(ATP)。将ATP核酸适配体序列分裂为P1和P2两个片段,在P1的5'端修饰荧光基团羧基荧光素(FAM),3'端巯基化修饰的P2通过Au-S键以自组装的方式修饰在AuNPs表面。未加入ATP时,P1与P2不结合,此时P1远离AuNPs,荧光信号较强;加入ATP后,可形成P1-ATP-P2的三明治结构,P15'端的FAM荧光基团靠近AuNPs,发生荧光共振能量转移,导致荧光信号减弱。对AuNPs与P2的结合、浓度比例以及加入P1和ATP后的稳定时间等实验条件进行了优化。在最优的条件下,传感器的荧光强度与ATP浓度在0.03~3.33 nmol/L和3.33~15 nmol/L浓度范围内呈两段的线性关系,检出限为0.03 nmol/L。该传感器对ATP表现出良好的特异性。

English


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  • 收稿日期:  2020-12-11
  • 修回日期:  2022-01-06
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