新型二维氮化石墨烯材料高选择性富集糖肽

李娟 李艳华

引用本文: 李娟, 李艳华. 新型二维氮化石墨烯材料高选择性富集糖肽[J]. 分析化学, 2021, 49(9): 1555-1562,1596. doi: 10.19756/j.issn.0253-3820.201724 shu
Citation:  LI Juan,  LI Yan-Hua. Selective Enrichment of Glycopeptides Using Two-Dimentional Novel Nitrogenated Graphene Materials[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1555-1562,1596. doi: 10.19756/j.issn.0253-3820.201724 shu

新型二维氮化石墨烯材料高选择性富集糖肽

    通讯作者: 李艳华,E-mail:m17709870057_1@163.com
  • 基金项目:

    大连医科大学附属第二医院2018年度"个体化诊疗协同创新中心"联合基金青年项目(No.dy2yhws201807)资助。

摘要: 糖蛋白的分离和鉴定对研究其生物功能、筛选生物标记物和研发新型生物药等具有重要的意义。由于生物样品成分复杂多样,以及糖蛋白较低的丰度,使得糖蛋白的分离和鉴定十分困难,因此,发展高效的糖肽富集方法非常重要。新型二维材料氮化石墨烯(C2N)具有高比表面积、良好的亲水性和丰富的氮,为糖肽富集提供了可能。本研究制备了C2N材料用于糖肽的选择性富集。所合成的C2N孔径为2.5 nm,比表面积为633 m2/g。基于C2N的糖肽富集方法具有高选择性(能从糖蛋白与牛血清白蛋白质量比为1∶200倍的酶解液中选择性富集糖肽)、高吸附量(150 mg/g)和高回收率(82.2%±7.0%)等特点。将此方法应用于100 μg HeLa细胞裂解液的3个技术重复样品的鉴定,分别得到来源于328、320和316条糖肽的339、353和327个糖基化位点。本研究为糖蛋白组学提供了新的技术,也为潜在肿瘤标记物的发现奠定了方法学基础。

English


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  • 收稿日期:  2020-12-03
  • 修回日期:  2021-07-06
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