Citation: XU Feng-Qin, ZHU Qun-Yan, ZHANG Hua, WANG Zhen-Xin. Preparation of Colloidal Gold-Labeled Immunochromatographic Strips for Detection of Soluble Interleukin-2 Receptors in Real Samples[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(6): 973-981. doi: 10.19756/j.issn.0253-3820.201723 shu

Preparation of Colloidal Gold-Labeled Immunochromatographic Strips for Detection of Soluble Interleukin-2 Receptors in Real Samples

XU Feng-Qin ^1,2 ,
ZHU Qun-Yan ¹ ,
ZHANG Hua ¹ ,
WANG Zhen-Xin ^1,2,,

1.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
School of Applied Chemical Engineering, University of Science and Technology of China, Hefei 230026, China

Corresponding author: WANG Zhen-Xin, wangzx@ciac.ac.cn
Received Date: 3 December 2020
Revised Date: 18 March 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.61901438).

Rapid and sensitive detection of soluble interleukin-2 receptor (sIL-2R) in serum is believed to be of vital importance in clinical diagnosis. In this study, a colloidal gold-labeled immunochromatographic strip was developed for rapid and quantitative detection of sIL-2R in real sample. The goat anti-rabbit secondary antibody (2ndAb) and rabbit anti-mouse sIL-2R monoclonal antibody (cAb) were sprayed onto the nitrocellulose membrane as control line (C line) and test line (T line), respectively. The rabbit anti-mouse sIL-2R monoclonal antibody (dAb) modified gold nanoparticles were used as markers to fabricate the immunochromatographic strip for detection of sIL-2R by a sandwich method. Three gold nanoparticles with different sizes and morphologies were synthesized, and their effects on the detection performance of colloidal gold-labeled immunochromatographic strip were investigated. The experimental results showed that the detection performance of colloidal gold-labeled immunochromatographic strip for sIL-2R was closely related to the morphology and particle size of gold nanoparticles. Among them, gold nanorod (GNR)-based immunochromatographic strip had the widest linear range (1-6250 ng/mL) and the lowest detection limit (1.0 ng/mL). In addition, the concentrations of sIL-2R in the sera of triple-negative breast tumors (MDA-MB-231)-bearing BALB/c nude mice were successfully detected using GNR-based immunochromatographic strips, and it was found that the concentration of sIL-2R was positively correlated with tumor progression.
- Gold nanoparticles,
- Immunochromatography,
- Soluble interleukin-2 receptor,
- Malignant tumor
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