Citation: YANG Cheng, ZHANG Ya-Qi, LIN Dai-Qin, LIU Yang, SUN Bing-Bing. Chemiluminescence Sensor Based on Composite Functional Nucleic Acid for Detection of Ochratoxin A in Wine[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(4): 496-503. doi: 10.19756/j.issn.0253-3820.201705 shu

Chemiluminescence Sensor Based on Composite Functional Nucleic Acid for Detection of Ochratoxin A in Wine

1.
State Key Laboratory of Fine Chemicals, School of Chemistry, College of Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
2.
Jiangxi Product Quality Supervision Testing Institute, Nanchang 330052, China;
3.
Key Laboratory of Life Organophosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China

Corresponding author: YANG Cheng, LIN Dai-Qin,
Received Date: 25 November 2020
Revised Date: 14 December 2020

Fund Project: Supported by the National Natural Science Foundation of China (No. 201874080), the Fundamental Research Funds for the Central Universities (No. DUT17LAB18, DUT15RC(3)064), the Open Funds of the State Key Laboratory of Electroanalytical Chemistry (No. SKLEAC202105), and the Open Funds of the Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, China.

A chemiluminescence sensor, using ochratoxin A (OTA) aptamer as the activation switch of the split DNAzyme, is established to achieve high-sensitivity detection of OTA in wine. Guanine-rich oligonucleotide can fold into a parallel G-quadruplex in the presence of K⁺. Hemin as a ligand is able to specifically bind to parallel G-quadruplexes. Hemin-G-quadruplex complexes display peroxidase-like activity, named as DNAzyme. The activity of the DNAzyme can be measured by chemiluminescence via the H₂O₂ mediated-oxidation with luminol. Guanine-rich oligonucleotide splits into two fragments, and when they are near to each other, they can reform DNAzyme. OTA aptamer as a switch is inserted into the nucleic acid sequence of DNAzyme. In the absence of OTA, the unfolded OTA aptamer inhibits the split DNAzyme effectively self-assemble to form a structure with enzymatic activity. In the presence of OTA, OTA induces the aptamer to form an antiparallel G-quadruplex and draws the tail of the aptamer closer, so that the two fragments of split DNAzyme refold and complexes with hemin. Folding degree of the aptamer will directly affect the activity of DNAzyme. The high-sensitive detection of OTA can be obtained through monitoring the enhancing ratio of chemiluminescence intensity. The linear range of this method is 0.10-2.00 nmol/L, and the limit of detection is 0.10 nmol/L.
- Functional nucleic acid,
- Aptamer,
- Split DNAzyme,
- Chemiluminescence,
- Ochratoxin A
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
1. [1]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
2. [2]
  Borong Yu , Huijiao Zhang , Xinyu Zhang , Xiaoying Li , Shuming Chen , Zhangang Han . The Blue Elf in the Dark: Gradient Science Popularization Experiments on Chemiluminescence. University Chemistry, 2024, 39(9): 295-303. doi: 10.12461/PKU.DXHX202403107
3. [3]
  Jia-He Li , Yu-Ze Liu , Jia-Hui Ma , Qing-Xiao Tong , Jian-Ji Zhong , Jing-Xin Jian . 洛芬碱衍生物的合成、化学发光与重金属离子检测. University Chemistry, 2025, 40(6): 230-237. doi: 10.12461/PKU.DXHX202407080
4. [4]
  Yuyang Xu , Ruying Yang , Yanzhe Zhang , Yandong Liu , Keyi Li , Zehui Wei . Research Progress of Aflatoxins Removal by Modern Optical Methods. University Chemistry, 2024, 39(11): 174-181. doi: 10.12461/PKU.DXHX202402064
5. [5]
  Minna Ma , Yujin Ouyang , Yuan Wu , Mingwei Yuan , Lijuan Yang . Green Synthesis of Medical Chemiluminescence Reagents by Photocatalytic Oxidation. University Chemistry, 2024, 39(5): 134-143. doi: 10.3866/PKU.DXHX202310093
6. [6]
  Cun WANG , Shaohan XU , Yuqian ZHANG , Yaoyao ZHANG , Tao GONG , Rong WEN , Yuhang LIAO , Yanrong REN . Terbium complex electrochemiluminescent emitters: Synthesis and application in the detection of epinephrine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1351-1360. doi: 10.11862/CJIC.20240427
7. [7]
  Pan Li , Huguo Shen , Cong Hua , Jinjie Fang , Xiangying Chi , Quan Jiang , Zichen Feng , Ye Kang , Bin Zheng . Synthesis and Characterization of an Aggregation-Induced Emission-Active Organic Cage Molecule: A Proposed Comprehensive Chemistry Experiment. University Chemistry, 2025, 40(11): 337-345. doi: 10.12461/PKU.DXHX202502083
8. [8]
  Chengxin Chen , Hongfei Shi , Xiaoyan Cai , Liang Mao , Zhe Chen . Enhanced bifunctional photocatalytic performances for H₂ evolution and HCHO elimination with an S-scheme CoWO₄/CdIn₂S₄ heterojunction. Acta Physico-Chimica Sinica, 2025, 41(12): 100155-0. doi: 10.1016/j.actphy.2025.100155
9. [9]
  Tinghui AN , Dong XIANG , Jiaqi LI , Jiawei WANG , Shuming YU , Nan WANG , Kedi CAI . Research progress on the application of laser synthesis technology for electrochemical functional materials. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1731-1754. doi: 10.11862/CJIC.20240412
10. [10]
  Cunming Yu , Dongliang Tian , Jing Chen , Qinglin Yang , Kesong Liu , Lei Jiang . Chemistry “101 Program” Synthetic Chemistry Experiment Course Construction: Synthesis and Properties of Bioinspired Superhydrophobic Functional Materials. University Chemistry, 2024, 39(10): 101-106. doi: 10.12461/PKU.DXHX202408008
11. [11]
  Qin Li , Kexin Yang , Qinglin Yang , Xiangjin Zhu , Xiaole Han , Tao Huang . Illuminating Chlorophyll: Innovative Chemistry Popularization Experiment. University Chemistry, 2024, 39(9): 359-368. doi: 10.3866/PKU.DXHX202309059
12. [12]
  Ming ZHENG , Yixiao ZHANG , Jian YANG , Pengfei GUAN , Xiudong LI . Energy storage and photoluminescence properties of Sm³⁺-doped Ba_0.85Ca_0.15Ti_0.90Zr_0.10O₃ lead-free multifunctional ferroelectric ceramics. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 686-692. doi: 10.11862/CJIC.20230388
13. [13]
  Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351
14. [14]
  Yajun Jian , Quanguo Zhai , Quan Gu , Shengli Gao . Reconstruction and Practice of the Teaching Content of “Carbon Group Elements” in Inorganic Chemistry to Reflect Comprehensive Education Function. University Chemistry, 2024, 39(11): 96-107. doi: 10.12461/PKU.DXHX202403006
15. [15]
  Ying Zhang , Fang Ge , Zhimin Luo . AI-Driven Biochemical Teaching Research: Predicting the Functional Effects of Gene Mutations. University Chemistry, 2025, 40(3): 277-284. doi: 10.12461/PKU.DXHX202412104
16. [16]
  Xiaoxuan Yu , Wukun Liu . Practice of Ideological and Political Education in Medicinal Chemistry for Pharmacy Administration Major: A Case Study on the Discovery of Cisplatin’s Anticancer Function. University Chemistry, 2025, 40(4): 408-414. doi: 10.12461/PKU.DXHX202405200
17. [17]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-0. doi: 10.3866/PKU.WHXB202310046
18. [18]
  Xuyu WANG , Xinran XIE , Dengke CAO . Photoreaction characteristics and luminescence modulation in phosphine-anthracene-based Au(Ⅰ) and Ir(Ⅲ) complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1513-1522. doi: 10.11862/CJIC.20250113
19. [19]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(11): 0-0.
20. [20]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(1504)
HTML views(299)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142