Citation: SHEN Cong-Cong, WU Cheng-Ke, CHEN Yue-Hua, WANG Jian-Xiu, YANG Ming-Hui, ZHANG Hua. Advance in Novel Methods for Enrichment and Precise Analysis of Circulating Tumor Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(4): 483-495. doi: 10.19756/j.issn.0253-3820.201700 shu

Advance in Novel Methods for Enrichment and Precise Analysis of Circulating Tumor Cells

1.
College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China;
2.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Corresponding author: YANG Ming-Hui, ZHANG Hua,
Received Date: 24 November 2020
Revised Date: 7 January 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos.22004028, 21722501, 21803018), the Henan Special Support for High-Level Talents Central Plains Science and Technology Innovation Leading Talents (No.204200510006), and the Key Project of Science and Technology of Henan province, China (Nos.202102310139, 202102311071).

The dissemination of circulating tumor cells (CTCs) in peripheral blood is an important intermediate step for cancer metastasis. CTC can provide complete information of cell biology and thus has become one of the most promising targets of "liquid biopsy". However, due to the extremely rarity, complex environment and strong heterogeneity of CTC in peripheral blood, the development of efficient enrichment and separation methods are the key in the accurate detection of CTC. Current reported CTC enrichment and analysis techniques are mainly based on the physical or biological characteristics of CTC. These methods are commonly combined with the traditional techniques including density gradient centrifugation, superfine membrane filtration and immunomagnetic separation, the novel methods such as microfluidic chip separation and two-dimensional electrophoresis-field separation, and the analysis techniques such as reverse transcription-polymerase chain reaction, flow cytometry, immunocytochemistry, photoacoustic imaging, surface-enhancement Raman scattering and electrochemistry to realize the accurate detection of CTC. In this review, the recent progress on enrichment techniques and detection methods for CTC was summarized, also the challenge and their clinical application status were discussed, which could provide reference for further improvement of the technology of enrichment, accurate counting and molecular phenotype analysis for CTC, as well as the application in scientific research and clinical practice.
- Circulating tumor cells,
- Enrichment and isolation,
- Precise analysis,
- Liquid biopsy,
- Cancer metastases,
- Review
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