基于免疫反应抑制MIL-101(Fe)电催化活性的肌钙蛋白I电化学传感器

引用本文: 杨海珠, 褚亚茹, 汪庆祥, 丁彩凤, 高凤. 基于免疫反应抑制MIL-101(Fe)电催化活性的肌钙蛋白I电化学传感器[J]. 分析化学, 2021, 49(5): 789-797. doi: 10.19756/j.issn.0253-3820.201693 shu

Citation: YANG Hai-Zhu, CHU Ya-Ru, WANG Qing-Xiang, DING Cai-Feng, GAO Feng. An Electrochemical Biosensor for Cardiac Troponin I Based on Electrocatalytic Activity Suppression of MIL-101(Fe) by Immunoreaction[J]. Chinese Journal of Analytical Chemistry, 2021, 49(5): 789-797. doi: 10.19756/j.issn.0253-3820.201693 shu

基于免疫反应抑制MIL-101(Fe)电催化活性的肌钙蛋白I电化学传感器

杨海珠 ^1, ,
褚亚茹 ^1, ,
汪庆祥 ^1, ,
丁彩凤 ^2, ,
高凤 ^{1,2,
,}

1.
闽南师范大学化学化工与环境学院, 福建省现代分离分析科学与技术重点实验室, 漳州 363000;
2.
青岛科技大学肿瘤标志物传感分析教育部重点实验室, 青岛 266042

通讯作者: 高凤,E-mail:Fgao1981@126.com

基金项目:
国家自然科学基金项目（Nos.21802064，21275127）、福建省自然科学基金项目（Nos.2018J01435，2019J05108）和青岛科技大学肿瘤标志物传感分析教育部重点实验室开放课题资助。

摘要: 采用水热法合成了具有过氧化氢（H₂O₂）模拟酶活性的金属-有机框架材料MIL-101（Fe）。将MIL-101（Fe）和心肌肌钙蛋白I抗体（anti-cTnI）依次通过共价键合法固定在氨基化石墨烯（NH₂-GR）修饰玻碳电极表面，构建了基于MIL-101（Fe）/NH₂-GR复合材料的新型cTnI电化学传感界面。电化学实验结果表明，此传感界面上的anti-cTnI与目标物cTnI的免疫反应可显著降低MIL-101（Fe）对H₂O₂的电催化性能，从而实现对cTnI的免标记传感分析。在10 fg/mL~0.1 μg/mL浓度范围内，传感器催化电流差（ΔI）与cTnI浓度的负对数（-lgC_cTnI）呈良好的线性关系，线性方程为ΔI（μA）=0.705×lg（C_cTnI/（g/mL））-11.2（R=0.995），检出限为3.1 fg/mL（S/N=3）。将所构建的电化学传感器应用于人血清中cTnI的检测，回收率为96.0%~103.0%。此传感器具有良好的选择性和重现性。

关键词:

English

An Electrochemical Biosensor for Cardiac Troponin I Based on Electrocatalytic Activity Suppression of MIL-101(Fe) by Immunoreaction

1.
Department of Chemistry and Environment Science, Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, China;
2.
Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

Corresponding author: GAO Feng, Fgao1981@126.com

Received Date: 20 November 2020
Revised Date: 27 January 2021
Fund Project:
Supported by the National Natural Science Foundation of China (Nos.21802064, 21275127), the Natural Science Foundation of Fujian Province, China (Nos.2019J05108, 2018J01435), and the Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology.

Abstract: The metal-organic framework material of MIL-101(Fe) with H₂O₂ mimicking enzyme activity was synthesized by a typical hydrothermal method. MIL-101(Fe) and cardiac troponin I antibody (anti-cTnI) were then successively immobilized on the surface of glassy carbon electrode that was pre-modified with amino-modified graphene (NH₂-GR) through the covalent bonding. Thus, a novel MIL-101(Fe)/NH₂-GR-based electrochemical sensing interface for cTnI was constructed. Electrochemical experiments showed that the immunoreaction between anti-cTnI and target cTnI on the sensing interface significantly suppressed the electrocatalytic activity of electrode-confined MIL-101(Fe) to H₂O₂, realizing the label-free analysis of cTnI. Under the optimal conditions, the catalytic current difference (ΔI) and the negative logarithm of the cTnI concentration (-lgC_cTnI) showed a good linear relationship in the concentration range of 10 fg/mL-0.1 μg/mL, and the detection limit was 3.1 fg/mL (S/N=3). The constructed sensor was applicable for detection of cTnI in actual human serum samples, and the recoveries were 96.0%-103.0%. The sensor exhibited good selectivity and reproducibility.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于免疫反应抑制MIL-101(Fe)电催化活性的肌钙蛋白I电化学传感器

通讯作者: 高凤,E-mail:Fgao1981@126.com

English

An Electrochemical Biosensor for Cardiac Troponin I Based on Electrocatalytic Activity Suppression of MIL-101(Fe) by Immunoreaction

Corresponding author: GAO Feng, Fgao1981@126.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于免疫反应抑制MIL-101(Fe)电催化活性的肌钙蛋白I电化学传感器

通讯作者: 高凤,E-mail:Fgao1981@126.com

English

An Electrochemical Biosensor for Cardiac Troponin I Based on Electrocatalytic Activity Suppression of MIL-101(Fe) by Immunoreaction

Corresponding author: GAO Feng, Fgao1981@126.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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