光化学比色传感器阵列用于贝母的快速鉴别

引用本文: 贾明艳, 杨茂桦, 彭露琳, 张梅, 冯亮. 光化学比色传感器阵列用于贝母的快速鉴别[J]. 分析化学, 2021, 49(3): 424-431. doi: 10.19756/j.issn.0253-3820.201662 shu

Citation: JIA Ming-Yan, YANG Mao-Hua, PENG Lu-Lin, ZHANG Mei, FENG Liang. Rapid Discrimination of Fritillary Herbs by A Photochemical Colorimetric Sensor Array[J]. Chinese Journal of Analytical Chemistry, 2021, 49(3): 424-431. doi: 10.19756/j.issn.0253-3820.201662 shu

光化学比色传感器阵列用于贝母的快速鉴别

贾明艳 ^1, ,
杨茂桦 ^1, ,
彭露琳 ^1, ,
张梅 ^{1,
,} ,
冯亮 ^{2,
,}

1.
成都中医药大学药学院, 成都 611137;
2.
中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 大连 116023

通讯作者: 张梅,E-mail:zhangmei63@126.com; 冯亮,E-mail:fengl@dicp.ac.cn

基金项目:
国家自然科学基金项目（Nos.82003907，81774202）和成都中医药大学杏林学者学科人才科研提升计划项目（Nos.CXTD2018010，BSH2018011）资助。

摘要: 贝母类药材是常用中药材，均具有止咳化痰的功效，但疗效和价格却存在较大差异。在市场上，贝母类药材的混淆和掺伪十分常见，传统的性状鉴别和显微鉴别已不能满足实际需要，现代常规鉴别方法又普遍存在分析时间长、仪器要求高、成本高等不足。本研究基于“指示剂置换法”和“溶液pH值改变”两种显色原理，利用具有交叉响应的商品化指示剂，针对贝母中的有机酸类及核苷类成分构建了3×3的光化学比色传感器阵列，用于贝母类药材的快速鉴别。传感器阵列在不同贝母药材中的颜色改变模式不同，由此构成了贝母类药材各自独特的“颜色差减指纹图谱”。通过偏最小二乘判别分析（PLS-DA）实现了川贝母、湖北贝母、平贝母、伊贝母、浙贝母和贝母伪品的准确区分和快速鉴别。本研究为贝母类药材的快速鉴别和质量控制提供了一种操作简单、成本低廉的有效方法。

关键词:

English

Rapid Discrimination of Fritillary Herbs by A Photochemical Colorimetric Sensor Array

1.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
2.
CAS Key Laboratory of Separation Science and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: ZHANG Mei, ; FENG Liang,

Received Date: 08 November 2020
Revised Date: 06 January 2021
Fund Project:
National Natural Science Foundation of China (Nos. 82003907, 81774202) and the Chengdu University of Traditional Chinese Medicine Xinglin Scholar Discipline Talent Research Promotion Program (Nos. CXTD2018010, BSH2018011).

Abstract: Fritillaria is a kind of traditional Chinese medicine commonly used in China. Fritillaria has the effect of relieving cough and reducing phlegm, but its curative effect and price are quite different. In the market, the confusion and adulteration of Fritillaria medicinal materials are very common, the traditional character identification and microscopic identification cannot meet the actual needs, and the modern identification methods generally have the limitations of long analysis time, high instrument requirements, and high price. This work was based on the organic acids and nucleosides in Fritillaria, with the help of two color development principles, "indicator displacement" and "changes in pH", using commercial indicators with cross-response to build a 3×3 photochemical colorimetric sensor array for rapid identification of Fritillaria medicinal materials. The sensor array had different color change patterns in different fritillary herbs, which constituted the unique fingerprint of fritillary herbs. Partial least square discriminant analysis (PLS-DA) was used for distinguishing and identifying Chuanbeimu, Hubeibeimu, Pingbeimu, Yibeimu, Zhebeimu, fake Fritillaria, so as to realize the accurate and rapid distinguishing of Fritillaria. This work provided a simple and cost-effective method for the rapid identification and quality control of Fritillaria.

Key words:

Fritillary herbs
/ Discrimination
/ Colorimetric sensor array
/ Indicators displacement assay
/ Partial least squares discriminant analysis

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沈阳化工大学材料科学与工程学院沈阳 110142

光化学比色传感器阵列用于贝母的快速鉴别

通讯作者: 张梅,E-mail:zhangmei63@126.com; 冯亮,E-mail:fengl@dicp.ac.cn

English

Rapid Discrimination of Fritillary Herbs by A Photochemical Colorimetric Sensor Array

Corresponding author: ZHANG Mei, ; FENG Liang,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

光化学比色传感器阵列用于贝母的快速鉴别

通讯作者: 张梅,E-mail:zhangmei63@126.com; 冯亮,E-mail:fengl@dicp.ac.cn

English

Rapid Discrimination of Fritillary Herbs by A Photochemical Colorimetric Sensor Array

Corresponding author: ZHANG Mei, ; FENG Liang,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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