基于表面增强拉曼光谱技术快速检测铜离子

引用本文: 张嘉惠, 陈阳, 李静, 赵杰, 张振, 卢小泉. 基于表面增强拉曼光谱技术快速检测铜离子[J]. 分析化学, 2021, 49(3): 440-448. doi: 10.19756/j.issn.0253-3820.201658 shu

Citation: ZHANG Jia-Hui, CHEN Yang, LI Jing, ZHAO Jie, ZHANG Zhen, LU Xiao-Quan. Rapid Detection of Copper Ions of Simulated Wilson's Disease Urine Based on Surface-Enhanced Raman Spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2021, 49(3): 440-448. doi: 10.19756/j.issn.0253-3820.201658 shu

基于表面增强拉曼光谱技术快速检测铜离子

张嘉惠 ^1, ,
陈阳 ^1, ,
李静 ^1, ,
赵杰 ^1, ,
张振 ^{1,
,} ,
卢小泉 ^{1,2,
,}

1.
天津市分子光电科学重点实验室, 天津大学理学院化学系, 天津 300072;
2.
甘肃省生物电化学与环境分析重点实验室, 西北师范大学化学化工学院, 兰州 730070

通讯作者: 张振,E-mail:zhzhen@tju.edu.cn; 卢小泉,E-mail:luxq@nwnu.edu.cn

基金项目:
国家自然科学基金项目（Nos.21705117，21904095）资助。

摘要: 肝豆状核变性（WD）是一种常染色体隐性遗传疾病，以因铜（Cu）代谢障碍引起的肝硬化以及因基底节损害为主造成的脑变性为特征，是少数可治愈的神经性遗传疾病之一。本研究建立了一种基于表面增强拉曼光谱（SERS）技术检测Cu²⁺的方法。制备了形貌均一、热点密集的金纳米棒（AuNRs）基底，并在其表面修饰4-巯基吡啶（Mpy），由于Mpy中吡啶环上的氮原子存在孤对电子，Cu²⁺与其配位，并使其拉曼光谱发生变化，据此实现高灵敏度、高选择性检测Cu²⁺。优化了反应条件，在25℃、反应2 min的条件下，Cu²⁺的线性检测范围为4.9×10^-8~1×10^-4 mol/L，检出限为49 nmol/L。将本方法应用于人工尿液中Cu²⁺的检测，相对标准偏差<7.8%。本方法操作简单、快速准确、检测过程无损，在WD的早期临床诊断与治疗方面具有良好的应用潜力。

关键词:

English

Rapid Detection of Copper Ions of Simulated Wilson's Disease Urine Based on Surface-Enhanced Raman Spectroscopy

ZHANG Jia-Hui ^1, ,
CHEN Yang ^1, ,
LI Jing ^1, ,
ZHAO Jie ^1, ,
ZHANG Zhen ^{1,
,} ,
LU Xiao-Quan ^{1,2,
,}

1.
Tianjin Key Laboratory of Molecular Optoelectronic Science, Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China;
2.
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China

Corresponding author: ZHANG Zhen, ; LU Xiao-Quan,

Received Date: 06 November 2020
Revised Date: 21 December 2020
Fund Project:
National Natural Science Foundation of China (Nos.21705117, 21904095).

Abstract: Wilson's disease (WD) is an autosomal recessive inherited disease. It is characterized by liver cirrhosis and brain degeneration caused by copper (Cu) metabolic disorder, which is one of the few neurogenetic diseases that can be treated, but improper treatment will cause disability or even death. This research constructed a method for detecting Cu²⁺ based on surface-enhanced Raman spectroscopy (SERS). The gold nanorods (AuNRs) substrates modified with 4-mercaptopyridine (Mpy) with uniform morphology and dense hot spots were prepared. Due to the existence of lone pairs of electrons in the nitrogen atom (N) on the pyridine ring, Cu²⁺ could coordinate with it to change the Raman spectrum of Mpy and realized the detection of Cu²⁺ with high sensitivity and selectivity. Under the condition of 25℃ and reaction time of 2 min, the linear range of detection was 4.9×10^-8-1×10^-4 mol/L, with a detection limit of 49 nmol/L(S/N=3). This method was applied to the detection of Cu²⁺ in artificial urine, and the relative standard deviation (RSD) was less than 7.8%. The presented method here was simple, fast and accurate, and the detection process was nondestructive. And it had the potential to be applied to the early diagnosis and treatment of WD in the clinic.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于表面增强拉曼光谱技术快速检测铜离子

通讯作者: 张振,E-mail:zhzhen@tju.edu.cn; 卢小泉,E-mail:luxq@nwnu.edu.cn

English

Rapid Detection of Copper Ions of Simulated Wilson's Disease Urine Based on Surface-Enhanced Raman Spectroscopy

Corresponding author: ZHANG Zhen, ; LU Xiao-Quan,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于表面增强拉曼光谱技术快速检测铜离子

通讯作者: 张振,E-mail:zhzhen@tju.edu.cn; 卢小泉,E-mail:luxq@nwnu.edu.cn

English

Rapid Detection of Copper Ions of Simulated Wilson's Disease Urine Based on Surface-Enhanced Raman Spectroscopy

Corresponding author: ZHANG Zhen, ; LU Xiao-Quan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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