基于铕纳米荧光配合物羧基活化原理的手印显现方法

引用本文: 朱中旭, 王猛, 李明, 袁传军, 吴建. 基于铕纳米荧光配合物羧基活化原理的手印显现方法[J]. 分析化学, 2021, 49(2): 237-245. doi: 10.19756/j.issn.0253-3820.201647 shu

Citation: ZHU Zhong-Xu, WANG Meng, LI Ming, YUAN Chuan-Jun, WU Jian. Europium Nanocomplex for Development of Latent Fingerprints Based on Carboxyl Activation Mechanism[J]. Chinese Journal of Analytical Chemistry, 2021, 49(2): 237-245. doi: 10.19756/j.issn.0253-3820.201647 shu

基于铕纳米荧光配合物羧基活化原理的手印显现方法

朱中旭 ^1, ,
王猛 ^{1,2,
,} ,
李明 ^1,2, ,
袁传军 ^1,2, ,
吴建 ^{3,
,}

1.
中国刑事警察学院刑事科学技术学院, 沈阳 110035;
2.
中国刑事警察学院新时代犯罪治理研究中心, 沈阳 110035;
3.
中国科学院宁波材料技术与工程研究所, 宁波 315201

通讯作者: 王猛,E-mail:mengwang@alum.imr.ac.cn; 吴建,E-mail:jwu@nimte.ac.cn

基金项目:
国家自然科学基金项目（Nos.21205139，21802169）、公安部科技强警基础工作专项（Nos.2014GABJC033，2018GABJC07，2018GABJC09）、辽宁省高等学校创新人才支持计划项目（No.LR2017055）和中国刑事警察学院2020年度研究生创新能力提升项目（No.2020YCYB29）资助。

摘要: 基于羧基活化原理，建立了手印纳米荧光显现方法，其显现效果和显现效率均优于传统化学键合手印显现方法。以铕离子（Eu³⁺）为发光中心、对苯二甲酸（PTA）为第一配体、邻菲罗啉（o-Phen）为第二配体，采用化学方法一步合成出表面羧基修饰的铕纳米荧光配合物。使用透射电子显微镜、X射线衍射、紫外可见吸收光谱、荧光光谱、傅里叶变换红外光谱、热重-差示扫描量热分析等技术对纳米配合物的微观形貌、结晶性能、吸收性质、荧光性能、表面基团和热学性质等进行了表征。利用1-（3-二甲氨基丙基）-3-乙基碳二亚胺盐酸盐（EDC）联合N-羟基琥珀酰亚胺（NHS）对纳米配合物表面羧基进行活化，使活化羧基与手印物质中胺基迅速发生酰胺反应，进而将纳米配合物与手印物质相偶联，最终实现光滑非渗透性客体表面潜在手印的高质量和高效率显现。对手印显现机理进行了深入讨论，对纳米配合物显现试剂的组成及手印显现时间进行了系统优化，并从对比度、灵敏度、选择性等角度全面考察了手印显现的效果。

关键词:

手印
/ 显现
/ 稀土
/ 荧光
/ 纳米材料
/ 铕配合物

English

Europium Nanocomplex for Development of Latent Fingerprints Based on Carboxyl Activation Mechanism

1.
College of Forensic Sciences, Criminal Investigation Police University of China, Shenyang 110035, China;
2.
Research Centre of Crime Governance in the New Era, Criminal Investigation Police University of China, Shenyang 110035, China;
3.
Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China

Corresponding author: WANG Meng, ; WU Jian,

Received Date: 03 November 2020
Revised Date: 22 November 2020
Fund Project:
Supported by the National Natural Science Foundation of China (Nos.21205139, 21802169), the Special Project for Reinforcing the Police by Science and Technology from the Ministry of Public Security of China (Nos.2014GABJC033, 2018GABJC07, 2018GABJC09), and the Program for Liaoning Innovative Talents in University (No.LR2017055).

Abstract: An activated carboxyl-based approach for latent fingerprint development with high quality and high efficiency was proposed. Firstly, carboxyl-functionalized fluorescent europium nanocomplex was chemically synthesized via a one-step process by using europium （Ⅲ） ion as the luminescence center, phthalic acid as the first ligand, and phenanthroline as the second ligand. Then, the micromorphology, crystal structure, ultraviolet absorption property, fluorescent emission performance, surface functional groups and thermal property of these synthesized europium nanocomplex were characterized by transmission electron microscopy, powder X-ray diffractometer, ultraviolet-visible spectrophotometer, fluorescence spectrophotometer, Fourier transform infrared spectrometer and simultaneous thermal analyzer, respectively. The carboxyl groups on the surface of europium nanocomplex were further activated using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride combined with N-hydroxysuccinimide. The activated carboxyl groups on the surface of europium nanocomplex could easily react with the amino groups in latent fingerprint through amide reaction, and the latent fingerprint was thus fluorescently developed with high efficiency. The mechanism of latent fingerprint development based on activated carboxyl process was discussed in detail, and the nanocomplex dosage and the staining time in developing suspension were also systematically optimized. In addition, the effects in fingerprint development were investigated from three aspects including contrast, sensitivity and selectivity. Experimental results showed that, the activated carboxyl-based approach could achieve a high performance in latent fingerprint development, exhibiting high efficiency, good operability and wide applicability.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于铕纳米荧光配合物羧基活化原理的手印显现方法

通讯作者: 王猛,E-mail:mengwang@alum.imr.ac.cn; 吴建,E-mail:jwu@nimte.ac.cn

English

Europium Nanocomplex for Development of Latent Fingerprints Based on Carboxyl Activation Mechanism

Corresponding author: WANG Meng, ; WU Jian,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于铕纳米荧光配合物羧基活化原理的手印显现方法

通讯作者: 王猛,E-mail:mengwang@alum.imr.ac.cn; 吴建,E-mail:jwu@nimte.ac.cn

English

Europium Nanocomplex for Development of Latent Fingerprints Based on Carboxyl Activation Mechanism

Corresponding author: WANG Meng, ; WU Jian,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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