10种野生植物果实对<i>α</i>-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究

引用本文: 田丝竹, 李绪文, 臧爽, 张子微, 张寒琦, 于永. 10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究[J]. 分析化学, 2021, 49(3): 449-459. doi: 10.19756/j.issn.0253-3820.201629 shu

Citation: TIAN Si-Zhu, LI Xu-Wen, ZANG Shuang, ZHANG Zi-Wei, ZHANG Han-Qi, YU Yong. Investigation of α-Amylase and Tyrosinase Inhibitory Activities, Phenolic Compounds, and Antioxidant Activity in Ten Kinds of Wild Fruits[J]. Chinese Journal of Analytical Chemistry, 2021, 49(3): 449-459. doi: 10.19756/j.issn.0253-3820.201629 shu

10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究

田丝竹 ^1, ,
李绪文 ^1, ,
臧爽 ^1, ,
张子微 ^1, ,
张寒琦 ^1, ,
于永 ^{2,
,}

1.
吉林大学化学学院, 长春 130012;
2.
吉林大学仪器与电气工程学院, 长春 130061

通讯作者: 于永,E-mail:analchem_JLU@163.com

基金项目:
吉林省教育厅“十三五”科学技术研究项目（No.2016-409）和吉林省科技发展计划项目（Nos.20180201059SF，20180201027GX）资助。

摘要: 考察了10种可食用野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用，结果表明，山荆子果实提取物对α-淀粉酶的抑制能力最强，其IC₅₀值与常见的α-淀粉酶抑制剂阿卡波糖接近。因此，山荆子果提实取物有望用于治疗非胰岛素依赖型糖尿病。采用1，1-二苯基苦基苯肼（DPPH）法测定了水果的抗氧化活性，采用Folin-Ciocalteu比色法和pH示差法测定总酚含量和总花青素含量，采用高效液相色谱（HPLC）法测定7种酚酸的含量。结果表明，10种野生水果对酪氨酸酶的抑制能力与其总酚含量和抗氧化活性均高度相关。冷冻野生水果中总酚的含量范围为617.3~7447.4 μg/g，其中，8种野生水果显示出高抗氧化能力。新鲜水果和冷冻水果的抗氧化能力之间的显著相关性表明，在液氮中快速冷冻水果并磨粉的保存方法对野生水果的抗氧化活性影响较小。

关键词:

English

Investigation of α-Amylase and Tyrosinase Inhibitory Activities, Phenolic Compounds, and Antioxidant Activity in Ten Kinds of Wild Fruits

1.
College of Chemistry, Jilin University, Changchun 130012, China;
2.
College of Instrumentation and Electrical Engineering, Jilin University, Changchun 130061, China

Corresponding author: YU Yong, analchem_JLU@163.com

Received Date: 27 October 2020
Revised Date: 20 December 2020
Fund Project:
Thirteenth Five-Year Program for Science and Technology of Education Department of Jilin Province (No. 2016-409) and the Science and Technology Development Planning Project of Jilin Province (Nos. 20180201059SF, 20180201027GX).

Abstract: Ten kinds of edible wild fruits from China, especially northeast China, were analyzed for their α-amylase and tyrosinase inhibitory activities. The extract of the fruit of dwarf apple had the highest α-amylase inhibitory activities and its IC₅₀ value was close to that of acarbose, which indicated that the extract of the fruit of dwarf apple could be a good candidate for the treatment of non-insulin dependent diabetes. The antioxidant capacity was determined using DPPH method. The total phenolics and total anthocyanins were determined by Folin-Ciocalteu method and pH-differential method, respectively. The contents of seven phenolic acids were determined by high-performance liquid chromatography (HPLC). The tyrosinase inhibitory activity was found to be highly correlated with both total phenolic content and antioxidant capacity. The contents of total phenolics in the frozen wild fruits ranged from 617.3 to 7447.4 μg/g. Eight out of ten wild fruits showed high antioxidant capacity. A significant correlation between the antioxidant capacity of raw (fresh) and frozen fruits indicated that fast freezing fruit powder in liquid nitrogen followed by fast thawing did not damage the antioxidant properties of the wild fruits.

Key words:

Wild fruits
/ α-Amylase inhibitory activity
/ Tyrosinase inhibitory activity
/ Phenolic acids
/ High performance liquid chromatography

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沈阳化工大学材料科学与工程学院沈阳 110142

10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究

通讯作者: 于永,E-mail:analchem_JLU@163.com

English

Investigation of α-Amylase and Tyrosinase Inhibitory Activities, Phenolic Compounds, and Antioxidant Activity in Ten Kinds of Wild Fruits

Corresponding author: YU Yong, analchem_JLU@163.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究

通讯作者: 于永,E-mail:analchem_JLU@163.com

English

Investigation of α-Amylase and Tyrosinase Inhibitory Activities, Phenolic Compounds, and Antioxidant Activity in Ten Kinds of Wild Fruits

Corresponding author: YU Yong, analchem_JLU@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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