液相色谱-质谱联用结合化学计量学方法分析冠心病及冠心病合并高血压患者的代谢特征

杨丽娟 丁筱雪 任繁栋 蔡芳 付光辉 任达兵 易伦朝 张宏

引用本文: 杨丽娟, 丁筱雪, 任繁栋, 蔡芳, 付光辉, 任达兵, 易伦朝, 张宏. 液相色谱-质谱联用结合化学计量学方法分析冠心病及冠心病合并高血压患者的代谢特征[J]. 分析化学, 2021, 49(10): 1649-1656. doi: 10.19756/j.issn.0253-3820.201572 shu
Citation:  YANG Li-Juan,  DING Xiao-Xue,  REN Fan-Dong,  CAI Fang,  FU Guang-Hui,  REN Da-Bing,  YI Lun-Zhao,  ZHANG Hong. Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension[J]. Chinese Journal of Analytical Chemistry, 2021, 49(10): 1649-1656. doi: 10.19756/j.issn.0253-3820.201572 shu

液相色谱-质谱联用结合化学计量学方法分析冠心病及冠心病合并高血压患者的代谢特征

    通讯作者: 易伦朝,E-mail:yilunzhao@kust.edu.cn; 张宏,E-mail:zhkhzx@yeah.net
  • 基金项目:

    国家自然科学基金项目(No.21775058)和云南省应用基础研究项目(昆医联合专项)(No.2017FE468(-169))资助。

摘要: 以51例健康人、21例冠心病患者以及16例冠心病合并高血压患者的血浆为样本,采用超高效液相色谱-高分辨质谱联用技术,分析两类患者的血浆代谢特征。在定性和定量分析104个内源性代谢物的基础上,进行主成分分析、偏最小二乘-判别分析,并结合变量重要性投影与单因素方差分析,分别筛选出8、41和26种可区分健康人与冠心病患者、健康人与冠心病合并高血压患者、冠心病与冠心病合并高血压患者的潜在特征代谢物。代谢通路分析结果表明,在冠心病与冠心病合并高血压患者中,亚油酸等脂肪酸代谢途径以及苯丙氨酸、酪氨酸、色氨酸的生物合成等氨基酸代谢途径发生了明显变化。其中,氨基酸代谢在冠心病合并高血压患者中表现出更加显著的变化。

English


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